amaxa buffer Search Results


amaxa primary cell nucleofection  (Amaxa)
86
Amaxa amaxa primary cell nucleofection
(A) siRNA mediated knockdown of Nrf2 in normal chondrocytes (ages 32 to 45, n=4) was performed by <t>nucleofection</t> yielding an ~89% depletion of Nrf2 compared to control siRNA. Nrf2 and its target protein levels were measured by immunoblotting and β-tubulin served as the loading control. The band intensity was normalized to β-tubulin. Results shown are mean ± SD. (B) siRNA knockdown of Nrf2 was performed in normal chondrocytes (ages 42 to 55, n=4) and then the cells were treated with RTA-408 to test for the specificity of Nrf2 activation. After nucleofection, the transduced cells were treated with RTA-408 for 48 hours. Repeated measures ANOVA was used for statistical analysis.
Amaxa Primary Cell Nucleofection, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amaxa+buffer/buffer+cell+muscle+nucleofector+primary+smooth/pmc10524306-60-13-13
Average 86 stars, based on 1 article reviews
amaxa primary cell nucleofection - by Bioz Stars, 2026-09
86/100 stars
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amaxa v buffer  (Amaxa)
86
Amaxa amaxa v buffer
(A) siRNA mediated knockdown of Nrf2 in normal chondrocytes (ages 32 to 45, n=4) was performed by <t>nucleofection</t> yielding an ~89% depletion of Nrf2 compared to control siRNA. Nrf2 and its target protein levels were measured by immunoblotting and β-tubulin served as the loading control. The band intensity was normalized to β-tubulin. Results shown are mean ± SD. (B) siRNA knockdown of Nrf2 was performed in normal chondrocytes (ages 42 to 55, n=4) and then the cells were treated with RTA-408 to test for the specificity of Nrf2 activation. After nucleofection, the transduced cells were treated with RTA-408 for 48 hours. Repeated measures ANOVA was used for statistical analysis.
Amaxa V Buffer, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amaxa+buffer/amaxa+buffer+v/pmc12490240-420-12-12
Average 86 stars, based on 1 article reviews
amaxa v buffer - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier
amaxa nucleofection kit buffer  (Amaxa)
86
Amaxa amaxa nucleofection kit buffer
(A) siRNA mediated knockdown of Nrf2 in normal chondrocytes (ages 32 to 45, n=4) was performed by <t>nucleofection</t> yielding an ~89% depletion of Nrf2 compared to control siRNA. Nrf2 and its target protein levels were measured by immunoblotting and β-tubulin served as the loading control. The band intensity was normalized to β-tubulin. Results shown are mean ± SD. (B) siRNA knockdown of Nrf2 was performed in normal chondrocytes (ages 42 to 55, n=4) and then the cells were treated with RTA-408 to test for the specificity of Nrf2 activation. After nucleofection, the transduced cells were treated with RTA-408 for 48 hours. Repeated measures ANOVA was used for statistical analysis.
Amaxa Nucleofection Kit Buffer, supplied by Amaxa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amaxa+buffer/amaxa+buffer+kit+nucleofection/10__1186_slash_s44330___025___00044___5-100-15-15
Average 86 stars, based on 1 article reviews
amaxa nucleofection kit buffer - by Bioz Stars, 2026-09
86/100 stars
  Buy from Supplier

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(A) siRNA mediated knockdown of Nrf2 in normal chondrocytes (ages 32 to 45, n=4) was performed by nucleofection yielding an ~89% depletion of Nrf2 compared to control siRNA. Nrf2 and its target protein levels were measured by immunoblotting and β-tubulin served as the loading control. The band intensity was normalized to β-tubulin. Results shown are mean ± SD. (B) siRNA knockdown of Nrf2 was performed in normal chondrocytes (ages 42 to 55, n=4) and then the cells were treated with RTA-408 to test for the specificity of Nrf2 activation. After nucleofection, the transduced cells were treated with RTA-408 for 48 hours. Repeated measures ANOVA was used for statistical analysis.

Journal: Osteoarthritis and cartilage

Article Title: Age and oxidative stress regulate Nrf2 homeostasis in human articular chondrocytes

doi: 10.1016/j.joca.2023.05.004

Figure Lengend Snippet: (A) siRNA mediated knockdown of Nrf2 in normal chondrocytes (ages 32 to 45, n=4) was performed by nucleofection yielding an ~89% depletion of Nrf2 compared to control siRNA. Nrf2 and its target protein levels were measured by immunoblotting and β-tubulin served as the loading control. The band intensity was normalized to β-tubulin. Results shown are mean ± SD. (B) siRNA knockdown of Nrf2 was performed in normal chondrocytes (ages 42 to 55, n=4) and then the cells were treated with RTA-408 to test for the specificity of Nrf2 activation. After nucleofection, the transduced cells were treated with RTA-408 for 48 hours. Repeated measures ANOVA was used for statistical analysis.

Article Snippet: Cells were harvested for nucleofection using collagnease and pronase, centrifuged, and resuspended in Amaxa primary cell nucleofection reagent.

Techniques: Knockdown, Control, Western Blot, Activation Assay