Journal:

Article Title: Fluorescent LYVE-1 antibody to image lymphatic trafficking of cancer cells

doi: 10.1016/j.jss.2007.12.769

Figure Lengend Snippet: Fluorescence imaging of in vivo murine lymphatic system of the anterior abdominal wall after injection of AlexaFluor-conjugated LYVE-1 to the inguinal lymph node. The inguinal (a, large arrow) and axillary (a, small arrow) lymph nodes, as well as the connecting lymphatics of the anterior abdominal wall (a-c) reveal durable fluorescent staining after delivery of AlexaFluor-conjugated monoclonal anti-mouse LYVE-1 antibody. Neighboring blood vessels did not stain (b, c).

Article Snippet: The cells were slowly directly injected into the area around the inguinal lymph node using a 1ml 27G2 latex free syringe (Becton Dickinson) four hours after administration of AlexaFluor-labeled monoclonal anti-mouse LYVE-1 as described above.

Techniques: Fluorescence, Imaging, In Vivo, Injection, Staining

Journal:

Article Title: Fluorescent LYVE-1 antibody to image lymphatic trafficking of cancer cells

doi: 10.1016/j.jss.2007.12.769

Figure Lengend Snippet: In vivo time course of fluorescence imaging of mouse lymphatics labeled with a one-time dose of fluorescent-labeled anti-LYVE1 antibody versus control fluorescent-labeled IgG or FITC-dextran. The hours post-delivery are denoted to the left of the images. All scale bars represent 0.1mm. While there is comparable fluorescence within the lymphatics immediately after antibody or FITC delivery (a-c), after four hours the unbound compound has washed away, leaving a strong positive signal only within the LYVE-1 labeled lymphatics (d-f). The small amount of nonspecific binding present within the conjugated IgG lymphatics at 4 hours post delivery (e) was completely gone by 12 hours after delivery (h). After a single administration of conjugated anti-LYVE1 antibody, the fluorescent signal remained present for up to 48 hours in the living animal (p). See the Materials and Methods section for antibody labeling procedures and means of in vitro delivery.

Article Snippet: The cells were slowly directly injected into the area around the inguinal lymph node using a 1ml 27G2 latex free syringe (Becton Dickinson) four hours after administration of AlexaFluor-labeled monoclonal anti-mouse LYVE-1 as described above.

Techniques: In Vivo, Fluorescence, Imaging, Labeling, Binding Assay, Antibody Labeling, In Vitro

Journal:

Article Title: Fluorescent LYVE-1 antibody to image lymphatic trafficking of cancer cells

doi: 10.1016/j.jss.2007.12.769

Figure Lengend Snippet: Fluorescent images of nude mouse axillary lymph node (a-c) and mesentery (d) stained with AlexaFluor-labeled anti-LYVE1 monoclonal antibody. Stained lymphatic tissue was imaged using a fluorescent inverted microscope at 10× (d), 20× (c) and 40× (a, b). Fluorescence microscopy showed staining of lymphatic vessels at the lymph node periphery with sparing of the follicles (a-c). Lymphatic vessels showed positive fluorescence while neighboring arteries and veins within the tissue did not stain (d). Binding of conjugated LYVE-1 to the lymphatic endothelium in vivo outlines vessel walls and valves (e, f).

Article Snippet: The cells were slowly directly injected into the area around the inguinal lymph node using a 1ml 27G2 latex free syringe (Becton Dickinson) four hours after administration of AlexaFluor-labeled monoclonal anti-mouse LYVE-1 as described above.

Techniques: Staining, Labeling, Inverted Microscopy, Fluorescence, Microscopy, Binding Assay, In Vivo

Journal:

Article Title: Fluorescent LYVE-1 antibody to image lymphatic trafficking of cancer cells

doi: 10.1016/j.jss.2007.12.769

Figure Lengend Snippet: Sequential images of cancer cells traveling through anterior abdominal wall lymphatics. Following administration of conjugated LYVE-1, the pancreatic cancer cell line XPA-1 RFP was injected into the inguinal lymph node. Red fluorescent XPA-1 cells, both individually (small arrows) and in clusters could be seen traveling through the fluorescent LYVE-1 labeled lymphatics. (a-f). RFP labeled cancer cells could also be seen collecting in the axillary lymph node after labeling of the node and lymphatics with green fluorescent LYVE-1 antibody (g).

Article Snippet: The cells were slowly directly injected into the area around the inguinal lymph node using a 1ml 27G2 latex free syringe (Becton Dickinson) four hours after administration of AlexaFluor-labeled monoclonal anti-mouse LYVE-1 as described above.

Techniques: Injection, Labeling