Journal: Nucleic Acids Research

Article Title: Lomofungin and dilomofungin: inhibitors of MBNL1-CUG RNA binding with distinct cellular effects

doi: 10.1093/nar/gku275

Figure Lengend Snippet: HTRF (black squares) and AlphaScreen (open circles) assays showed inhibition of biotin-CUG 12 binding to MBNL1-Δ105-His 6 protein by lomofungin. Subsequent analyses indicated two molecular species in the lomofungin stock. Error bars represent 1 s.d.

Article Snippet: The confirmatory assay employed a different detection system, consisting of AlphaScreen donor beads (conjugated to streptavidin) that emitted a singlet oxygen to activate AlphaScreen acceptor beads (coated with nickel, PerkinElmer).

Techniques: Amplified Luminescent Proximity Homogenous Assay, Inhibition, Binding Assay

Journal: Nucleic Acids Research

Article Title: Lomofungin and dilomofungin: inhibitors of MBNL1-CUG RNA binding with distinct cellular effects

doi: 10.1093/nar/gku275

Figure Lengend Snippet: AlphaScreen assay showing that dilomofungin (open triangles) is 17-fold more potent as inhibitor of MBNL1-(CUG) 12 binding than lomofungin (closed squares) [IC 50 s = 42 nM, 95% CI 33 to 53 nM, and 717 nM, 95% CI 533 to 964 nM, respectively].

Article Snippet: The confirmatory assay employed a different detection system, consisting of AlphaScreen donor beads (conjugated to streptavidin) that emitted a singlet oxygen to activate AlphaScreen acceptor beads (coated with nickel, PerkinElmer).

Techniques: Amplified Luminescent Proximity Homogenous Assay, Binding Assay

Journal: iScience

Article Title: A series of xanthenes inhibiting Rad6 function and Rad6-Rad18 interaction in the PCNA ubiquitination cascade

doi: 10.1016/j.isci.2022.104053

Figure Lengend Snippet:

Article Snippet: AlphaScreen FLAG (M2) detection kit , PerkinElmer , Cat#6760613.

Techniques: Virus, Recombinant, Ubiquitin Proteomics, Modification, Amplified Luminescent Proximity Homogenous Assay, Plasmid Preparation, Software