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Image Search Results
Journal: Scientific Reports
Article Title: The S . aureus 4-oxalocrotonate tautomerase SAR1376 enhances immune responses when fused to several antigens
doi: 10.1038/s41598-017-01421-z
Figure Lengend Snippet: Impact of fusion of SAR1376 mutant P1A to truncated Hla on immunogenicity. ( A ) Anti-AT antibody levels. ( B ) Anti-SAR1376 antibody levels after vaccination of BALB/c mice with tHla75-no scaffold, tHla75-P1A, or empty vectors (control) as assessed respectively by ELISA and LIPS assay. Sera were collected pre boost (day 56 post Adenovirus prime) and post MVA boost (day 70 post prime). Dotted line: threshold for background response. ( C ) AT neutralizing activity of the antibodies was assessed post boost by Toxin Neutralisation Assay. Assay minimum and maximum levels were determined with mAb 8B7 and recombinant AT as described in Methods. **p < 0.01.
Article Snippet:
Techniques: Mutagenesis, Immunopeptidomics, Control, Enzyme-linked Immunosorbent Assay, Lips Assay, Activity Assay, Recombinant