Journal: Biomolecules

Article Title: GDF-15 Inhibits ADP-Induced Human Platelet Aggregation through the GFRAL/RET Signaling Complex

doi: 10.3390/biom14010038

Figure Lengend Snippet: The expression of receptors associated with GDF-15 on platelets. ( A ) GFRAL and RET were detected in platelets. ( B ) TGF-βRI rather than TGF-βRII was expressed on platelets. ( C ) Among the three members of the EGFR family associated with GDF-15, EGFR, and HER3 were expressed on platelets. n = 6. Original images of ( A – C ) can be found in .

Article Snippet: According to a previously described method [ ], the GDF-15-related receptor microarray comprises five recombinant human proteins, including those rearranged during transfection (RET, 11997-H08H1, Sino Biological, Beijing, China), the epidermal growth factor receptor (EGFR, 10001-H08S, Sino Biological, Beijing, China), human epidermal growth factor receptor 3 (HER3, 10201-HCCH, Sino Biological, Beijing, China), TGF-βRI (10459-H02H, Sino Biological, Beijing, China), and GFRAL (9467-GR, R&D Systems, Minneapolis, MN, USA).

Techniques: Expressing

Journal: Biomolecules

Article Title: GDF-15 Inhibits ADP-Induced Human Platelet Aggregation through the GFRAL/RET Signaling Complex

doi: 10.3390/biom14010038

Figure Lengend Snippet: The screening for proteins that interact with GDF-15 among the receptors expressed on human platelets. ( A ) The results of the GDF-15-related receptor microarray detecting direct interactions between receptors (GFRAL, RET, EGFR, HER3, and TGF-βRI) and GDF-15 at 0.1 μg/mL, 1 μg/mL, and 10 μg/mL Biotin was used for the negative control. ( B ) The immunoprecipitation assay revealed an interaction between GDF-15 and GFRAL in human platelets. Rabbit IgG was used for isotype control. VeriBlot for the IP detection reagent, which only recognizes native (non-reduced) antibodies, was used for immunoblotting. Red box: the Western Blot band for GFRAL. Original images of ( B ) can be found in .

Article Snippet: According to a previously described method [ ], the GDF-15-related receptor microarray comprises five recombinant human proteins, including those rearranged during transfection (RET, 11997-H08H1, Sino Biological, Beijing, China), the epidermal growth factor receptor (EGFR, 10001-H08S, Sino Biological, Beijing, China), human epidermal growth factor receptor 3 (HER3, 10201-HCCH, Sino Biological, Beijing, China), TGF-βRI (10459-H02H, Sino Biological, Beijing, China), and GFRAL (9467-GR, R&D Systems, Minneapolis, MN, USA).

Techniques: Microarray, Negative Control, Immunoprecipitation, Western Blot