Journal: Cell reports

Article Title: DBC1 Regulates p53 Stability via Inhibition of CBP-Dependent p53 Polyubiquitination

doi: 10.1016/j.celrep.2019.02.076

Figure Lengend Snippet: (A) U2OS cells were untreated or treated with 2 μM doxorubicin, followed by p53 IP (left) from cytoplasmic and nuclear lysates, respectively, followed by immunoblotting. Lysates were calibrated for p53 levels by immunoblotting (right) to allow equal levels of p53 in each IP. See also . (B) U2OS cells were untreated or treated with 2 μM doxorubicin, 25 μM cisplatin, or 1 μM staurosporine for 6 h, followed by immunoblotting of cytoplasmic and nuclear lysates. See also . (C) CBP IPs from cytoplasmic and nuclear fractions in (B), followed by immunoblotting with CBP and DBC1 antibodies. (D) U2OS cells were untreated or treated with 2 μM doxorubicin or 1 μM staurosporine for 1 and 6 h, followed by qRT-PCR to determine DBC1 mRNA levels. Graph shows DBC1 mRNA levels relative to no treatment control. Error bars represent mean ± SEM from three independent experiments. RNA level was normalized to GAPDH. (E) Immunofluorescence of U2OS cells untreated or treated with 25 μM cisplatin, 2 μM doxorubicin, or 1 μM staurosporine for 6 h and stained with DBC1 antibody. (F) U2OS cells stably expressing control shRNA, CBP shRNA, DBC1 shRNA, or CBP-DBC1 shRNAs were treated with 25 μM cisplatin for 24 h, followed by cell permeabilization, staining with anti-cleaved caspase-3 antibody, and analysis by flow cytometry. Graph shows the percentage of cells with cleaved caspase-3. Error bars represent mean ± SEM from three independent experiments. (G) U2OS cells stably expressing control, CBP, DBC1, or CBP-DBC1 shRNAs were treated with 25 μM cisplatin for 24 h, followed by cell lysis and immunoblotting with the indicated antibodies. See also .

Article Snippet: Alexa Fluor 647 Rabbit anti-active caspase 3 , BD Biosciences , Cat. #560626; RRID:AB_1727414.

Techniques: Western Blot, Quantitative RT-PCR, Immunofluorescence, Staining, Stable Transfection, Expressing, shRNA, Flow Cytometry, Lysis

Journal: Cell reports

Article Title: DBC1 Regulates p53 Stability via Inhibition of CBP-Dependent p53 Polyubiquitination

doi: 10.1016/j.celrep.2019.02.076

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Alexa Fluor 647 Rabbit anti-active caspase 3 , BD Biosciences , Cat. #560626; RRID:AB_1727414.

Techniques: shRNA, Plasmid Preparation, Negative Control, Recombinant, Mass Spectrometry, Software

Journal: Journal of Animal Science and Biotechnology

Article Title: Dietary arachidonate in milk replacer triggers dual benefits of PGE 2 signaling in LPS-challenged piglet alveolar macrophages

doi: 10.1186/s40104-019-0321-1

Figure Lengend Snippet: Staining reagents used in flow cytometry of immune cells isolated from lungs of milk replacer-fed pigs

Article Snippet: CD172A , 74–22-15A , IgG2b , Alexa 647 , Secondary antibody , BEI Resources , Southern Biotech.

Techniques: Staining, Flow Cytometry, Isolation, Labeling

Journal: Clinical and Vaccine Immunology : CVI

Article Title: Interactive Changes between Macrophages and Adipocytes ▿ †

doi: 10.1128/CVI.00494-09

Figure Lengend Snippet: Influence of peritoneal macrophages on the levels of adiponectin ( ACDC ), GLUT1 , and GLUT4 transcripts in 3T3-L1 adipocytes a

Article Snippet: A rabbit polyclonal antibody against mouse GLUT1 (catalog no. CBL242) was obtained from BD Biosciences (San Jose, CA).

Techniques:

Journal: Clinical and Vaccine Immunology : CVI

Article Title: Interactive Changes between Macrophages and Adipocytes ▿ †

doi: 10.1128/CVI.00494-09

Figure Lengend Snippet: Influence of cytokines on the regulation of the ACDC, GLUT1, and GLUT4 genes in 3T3-L1 adipocytes a

Article Snippet: A rabbit polyclonal antibody against mouse GLUT1 (catalog no. CBL242) was obtained from BD Biosciences (San Jose, CA).

Techniques: Cell Culture

Journal: Clinical and Vaccine Immunology : CVI

Article Title: Interactive Changes between Macrophages and Adipocytes ▿ †

doi: 10.1128/CVI.00494-09

Figure Lengend Snippet: Effects of recombinant TNF-α, IL-6, and IL-1β on GLUT4 protein expression. Fully differentiated adipocytes were incubated in medium containing recombinant mouse TNF-α (2 ng/ml), IL-6 (10 ng/ml), or IL-1β (20 ng/ml) for 2 days. Cells were fixed and immunostained for GLUT1 or GLUT4 expression using flow cytometry as described in Materials and Methods. The data are presented as means ± standard errors of the means for 3 to 6 independent replicates per treatment. Different letters indicate a significant difference. A P-value of <0.05 was considered significant.

Article Snippet: A rabbit polyclonal antibody against mouse GLUT1 (catalog no. CBL242) was obtained from BD Biosciences (San Jose, CA).

Techniques: Recombinant, Expressing, Incubation, Flow Cytometry