Journal: Frontiers in Immunology

Article Title: Ebosin Ameliorates Psoriasis-Like Inflammation of Mice via miR-155 Targeting tnfaip3 on IL-17 Pathway

doi: 10.3389/fimmu.2021.662362

Figure Lengend Snippet: Ebosin inhibited the inflammatory response and IL-17 signaling pathway. (A) RT-PCR analyses of cytokines in psoriasis-like mice without or with Methotrexate (MTX) and Ebosin. (B) RT-PCR analyses of tnfaip3 and tnip1 in psoriasis-like mice without or with MTX and Ebosin. (C) Western blotting analyses for proteins on IL-17 signaling pathway in psoriasis-like mice without or with MTX and Ebosin, including A20, Act1, TRAF6, and phosphor-p65. All data were presented as mean ± SD; n = 3. # P < 0.05, ## P < 0.01, ### P < 0.001 vs. control; * P < 0.05, ** P < 0.01, *** P < 0.001 vs. IMQ-induced vehicle group.

Article Snippet: To determine intracellular Foxp3 and IL-17A expression, cells were fixed and permeated by Transcription Factor Buffer Set (BD Pharmingen, 562574), and were then stained with anti-Foxp3-PE (1:250, BD Pharmingen, 563101) or anti–IL-17A-Alex647 (1:250, BD Pharmingen, 560184).

Techniques: Reverse Transcription Polymerase Chain Reaction, Western Blot