Journal: Bioactive Materials

Article Title: Mesenchymal stem cell activity across a graded scaffold-hydrogel composite biomaterial for tendon-to-bone enthesis repair

doi: 10.1016/j.bioactmat.2025.07.017

Figure Lengend Snippet: Histological evaluation of triphasic scaffold structure and matrix remodeling. FFPE sections of hMSC-seeded triphasic scaffolds (n = 2) at both Day 1 and Day 21 were stained with either trichrome, alizarin red, or alcian blue staining. Representative images are provided of (A) a scan of a full scaffold (trichrome only), (B) the osseous region, (C) the tendinous region, and (D) the interfacial region.

Article Snippet: Samples were stained by one of four staining procedures: hematoxylin/eosin (H&E, SL200 & SL201, StatLab), alizarin red (Cat# 2003999, EMD Millipore Corp.), trichrome (Cat# ab150686, Abcam), or alcian blue (pH 2.5, Cat# KTABP2.5, StatLab) with staining procedures followed according to manufacturer recommendation.

Techniques: Staining

Journal: Stem Cells International

Article Title: Curcumin-Activated Mesenchymal Stem Cells Derived from Human Umbilical Cord and Their Effects on MPTP-Mouse Model of Parkinson's Disease: A New Biological Therapy for Parkinson's Disease

doi: 10.1155/2020/4636397

Figure Lengend Snippet: Phenotypic characterization and differentiation of hUC-MSCs. (a) Flow cytometry showing that isolated cultured hUC-MSCs highly expressed CD105, CD73, and CD90 and were negative for hematopoietic markers CD19, CD34, and CD45 and for the lymphocyte surface marker HLA-DR. (b) (A) Gross morphology of hUC-MSCs under light microscopy, (B) oil-red-O staining for the adipogenic differentiation of hUC-MSCs after 3 weeks, (C) endogenous alkaline phosphatase staining for the osteogenic differentiation of hUC-MSCs, and (D) Alcian blue staining of hUC-MSCs after 3 weeks for chondrogenic differentiation (scale bar = 100 μ m).

Article Snippet: The sections were analyzed for chondrogenic differentiation using a commercially available Alcian blue kit (IHC World, Woodstock).

Techniques: Flow Cytometry, Isolation, Cell Culture, Marker, Light Microscopy, Staining

Journal: Molecular & Cellular Proteomics : MCP

Article Title: Cold Atmospheric Plasma Jet Promotes Wound Healing Through CK2-Coordinated PI3K/AKT and MAPK Signaling Pathways

doi: 10.1016/j.mcpro.2025.100962

Figure Lengend Snippet: Histological staining of extracellular matrix proteins in CAPJ-treated wound tissues. Rat skin cutaneous wound was treated with CAPJ for 60s. CAPJ-treated wound tissue section (CAPJ), with parallel untreated control (Ctrl), were prepared on day-3, -7, and -14, and subjected for histochemical staining by using Masson’s Trichrome Stain kit for collagen staining ( top panel, in dark blue color), Elastic Stain kit for elastin staining ( middle panel, in black color), and Alcian Blue Stain kit for hyaluronic acid (HA) staining ( button panel, in light-blue color). The red arrows were the extensively stained area for collagen and elastic fibers formation as well as the conjugates of HA and proteoglycans. Right panel correspond to high-magnification images of each cropped area on low-magnification image. Scale bars in low- and high-magnification images are 20 μm and 5 μm, respectively. CAPJ, cold atmospheric plasma jet.

Article Snippet: Tissue section (5 μm thick) was prepared in a standard fashion and observed in detail using Masson’s Trichrome Stain kit (StatLab, #KTMTR), Elastic Stain Kit (Modified Verhoeff's, ScyTek, ETS-1-IFU) and Alcian Blue (pH 2.5) Stain Kit (ScyTek, AFR-1-IFU).

Techniques: Staining, Control, Clinical Proteomics

Journal: Polymers

Article Title: Osteochondral Regeneration Induced by TGF-β Loaded Photo Cross-Linked Hyaluronic Acid Hydrogel Infiltrated in Fused Deposition-Manufactured Composite Scaffold of Hydroxyapatite and Poly (Ethylene Glycol)-Block-Poly(ε-Caprolactone)

doi: 10.3390/polym9050182

Figure Lengend Snippet: The histological staining (Alcian blue/PAS staining) and specimen of cartilage sections of rabbit after 12 weeks after implantation. ( A ) The control group with only bone formation; and ( B ) the experimental group showed bone and hyalin cartilage regeneration (arrow means defect); ( C ) The specimen of the experimental group showed good cartilage and bone regeneration on the surface layer and residual un-degraded scaffold in the deep layer.

Article Snippet: In histological analysis, rabbit articular cartilage sections were stained with Alcian blue and periodic acid-schiff (PAS) double-staining with a Polyscience Alcian Blue/PAS kit.

Techniques: Staining