akt2 Search Results


goat polyclonal antibody against akt2  (R&D Systems)
93
R&D Systems goat polyclonal antibody against akt2
Goat Polyclonal Antibody Against Akt2, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat polyclonal antibody against akt2 - by Bioz Stars, 2026-04
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adenoviruses expressing admyr akt2  (Vector Biolabs)
96
Vector Biolabs adenoviruses expressing admyr akt2
Adenoviruses Expressing Admyr Akt2, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti akt2 antibody for immunohistochemistry ihc  (R&D Systems)
90
R&D Systems anti akt2 antibody for immunohistochemistry ihc
Anti Akt2 Antibody For Immunohistochemistry Ihc, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti akt2 antibody for immunohistochemistry ihc - by Bioz Stars, 2026-04
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antihuman akt2 phycoerythrin monoclonal antibody  (R&D Systems)
90
R&D Systems antihuman akt2 phycoerythrin monoclonal antibody
Antihuman Akt2 Phycoerythrin Monoclonal Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
antihuman akt2 phycoerythrin monoclonal antibody - by Bioz Stars, 2026-04
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rabbit anti akt2  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc rabbit anti akt2
Rabbit Anti Akt2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
rabbit anti akt2 - by Bioz Stars, 2026-04
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akt  (Proteintech)
96
Proteintech akt
Akt, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/akt/product/Proteintech
Average 96 stars, based on 1 article reviews
akt - by Bioz Stars, 2026-04
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akt1  (Santa Cruz Biotechnology)
94
Santa Cruz Biotechnology akt1
Akt1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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pcdna3 myr ha akt3  (Addgene inc)
94
Addgene inc pcdna3 myr ha akt3
Pcdna3 Myr Ha Akt3, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp akt2 mm02026778 g1  (Thermo Fisher)
86
Thermo Fisher gene exp akt2 mm02026778 g1
Gene Exp Akt2 Mm02026778 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal anti mouse p akt2 ser474 ab  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc rabbit polyclonal anti mouse p akt2 ser474 ab
FIGURE 1. Macrophages become insulin resistant and do not uptake glucose upon stimulation. (A) Western blot analysis of p-Akt (Ser473) in nonstimulated and after 30 min of stimulation with 100 nM insulin for control and macrophages from short-term HFD–fed mice. Summary graph of average phosphorylation of Akt in all conditions (basal and after insulin stimulation) normalized to total Akt. (B) Expression of IR in control and macrophages from short-term HFD–fed mice, measured by real-time PCR and Western blot. <t>Akt2</t> macrophages are insulin resistant, as demonstrated by (C) Western blot analysis of Akt phosphorylation for WT (control) and Akt22/2 macrophages before and after insulin stimulation and (D) Akt2 phosphorylation p-Akt2 <t>(Ser474)</t> and (E) Akt1 phosphorylation p-Akt1 (Ser473) in macrophages from control and HFD-fed mice before and after 30 min of stimulation with 100 nM insulin. All graphs are representative of three to six independent experiments and show mean 6 SD. *p , 0.05, **p , 0.01, ***p , 0.001, insulin stimulation versus no stimulation. #p , 0.05, ##p , 0.01, ###p , 0.001, insulin-resistant versus control macrophages.
Rabbit Polyclonal Anti Mouse P Akt2 Ser474 Ab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gene exp akt2 hs01086102 m1  (Thermo Fisher)
90
Thermo Fisher gene exp akt2 hs01086102 m1
FIGURE 1. Macrophages become insulin resistant and do not uptake glucose upon stimulation. (A) Western blot analysis of p-Akt (Ser473) in nonstimulated and after 30 min of stimulation with 100 nM insulin for control and macrophages from short-term HFD–fed mice. Summary graph of average phosphorylation of Akt in all conditions (basal and after insulin stimulation) normalized to total Akt. (B) Expression of IR in control and macrophages from short-term HFD–fed mice, measured by real-time PCR and Western blot. <t>Akt2</t> macrophages are insulin resistant, as demonstrated by (C) Western blot analysis of Akt phosphorylation for WT (control) and Akt22/2 macrophages before and after insulin stimulation and (D) Akt2 phosphorylation p-Akt2 <t>(Ser474)</t> and (E) Akt1 phosphorylation p-Akt1 (Ser473) in macrophages from control and HFD-fed mice before and after 30 min of stimulation with 100 nM insulin. All graphs are representative of three to six independent experiments and show mean 6 SD. *p , 0.05, **p , 0.01, ***p , 0.001, insulin stimulation versus no stimulation. #p , 0.05, ##p , 0.01, ###p , 0.001, insulin-resistant versus control macrophages.
Gene Exp Akt2 Hs01086102 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp akt2 hs01086102 m1/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
gene exp akt2 hs01086102 m1 - by Bioz Stars, 2026-04
90/100 stars
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mouse anti akt2  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc mouse anti akt2
FIGURE 1. Macrophages become insulin resistant and do not uptake glucose upon stimulation. (A) Western blot analysis of p-Akt (Ser473) in nonstimulated and after 30 min of stimulation with 100 nM insulin for control and macrophages from short-term HFD–fed mice. Summary graph of average phosphorylation of Akt in all conditions (basal and after insulin stimulation) normalized to total Akt. (B) Expression of IR in control and macrophages from short-term HFD–fed mice, measured by real-time PCR and Western blot. <t>Akt2</t> macrophages are insulin resistant, as demonstrated by (C) Western blot analysis of Akt phosphorylation for WT (control) and Akt22/2 macrophages before and after insulin stimulation and (D) Akt2 phosphorylation p-Akt2 <t>(Ser474)</t> and (E) Akt1 phosphorylation p-Akt1 (Ser473) in macrophages from control and HFD-fed mice before and after 30 min of stimulation with 100 nM insulin. All graphs are representative of three to six independent experiments and show mean 6 SD. *p , 0.05, **p , 0.01, ***p , 0.001, insulin stimulation versus no stimulation. #p , 0.05, ##p , 0.01, ###p , 0.001, insulin-resistant versus control macrophages.
Mouse Anti Akt2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti akt2/product/Cell Signaling Technology Inc
Average 94 stars, based on 1 article reviews
mouse anti akt2 - by Bioz Stars, 2026-04
94/100 stars
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Image Search Results


FIGURE 1. Macrophages become insulin resistant and do not uptake glucose upon stimulation. (A) Western blot analysis of p-Akt (Ser473) in nonstimulated and after 30 min of stimulation with 100 nM insulin for control and macrophages from short-term HFD–fed mice. Summary graph of average phosphorylation of Akt in all conditions (basal and after insulin stimulation) normalized to total Akt. (B) Expression of IR in control and macrophages from short-term HFD–fed mice, measured by real-time PCR and Western blot. Akt2 macrophages are insulin resistant, as demonstrated by (C) Western blot analysis of Akt phosphorylation for WT (control) and Akt22/2 macrophages before and after insulin stimulation and (D) Akt2 phosphorylation p-Akt2 (Ser474) and (E) Akt1 phosphorylation p-Akt1 (Ser473) in macrophages from control and HFD-fed mice before and after 30 min of stimulation with 100 nM insulin. All graphs are representative of three to six independent experiments and show mean 6 SD. *p , 0.05, **p , 0.01, ***p , 0.001, insulin stimulation versus no stimulation. #p , 0.05, ##p , 0.01, ###p , 0.001, insulin-resistant versus control macrophages.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Insulin Resistance in Macrophages Alters Their Metabolism and Promotes an M2-Like Phenotype.

doi: 10.4049/jimmunol.1800065

Figure Lengend Snippet: FIGURE 1. Macrophages become insulin resistant and do not uptake glucose upon stimulation. (A) Western blot analysis of p-Akt (Ser473) in nonstimulated and after 30 min of stimulation with 100 nM insulin for control and macrophages from short-term HFD–fed mice. Summary graph of average phosphorylation of Akt in all conditions (basal and after insulin stimulation) normalized to total Akt. (B) Expression of IR in control and macrophages from short-term HFD–fed mice, measured by real-time PCR and Western blot. Akt2 macrophages are insulin resistant, as demonstrated by (C) Western blot analysis of Akt phosphorylation for WT (control) and Akt22/2 macrophages before and after insulin stimulation and (D) Akt2 phosphorylation p-Akt2 (Ser474) and (E) Akt1 phosphorylation p-Akt1 (Ser473) in macrophages from control and HFD-fed mice before and after 30 min of stimulation with 100 nM insulin. All graphs are representative of three to six independent experiments and show mean 6 SD. *p , 0.05, **p , 0.01, ***p , 0.001, insulin stimulation versus no stimulation. #p , 0.05, ##p , 0.01, ###p , 0.001, insulin-resistant versus control macrophages.

Article Snippet: Briefly, after blocking with 5% BSA PBS (pH 7.4) for an hour at room temperature, the membranes were incubated with rabbit polyclonal anti-mouse p-Akt (Ser473) Ab (Cell Signaling Technology), rabbit polyclonal anti-mouse Akt Ab (Cell Signaling Technology), rabbit polyclonal anti-mouse p-Akt2 (Ser474) Ab (Cell Signaling Technology), rabbit polyclonal anti-mouse Akt2 Ab (Cell Signaling Technology), rabbit polyclonal anti-mouse p-S6 (235/236) Ab (Cell Signaling Technology), rabbit polyclonal anti-mouse p–4E-BP1(Thr37/46) Ab (Cell Signaling Technology), rabbit polyclonal anti-mouse inducible NO synthase (iNOS) Ab (Abcam), goat polyclonal anti-mouse arginase 1 (Abcam), goat polyclonal anti-mouse Fizz1 Ab (Abcam), rabbit polyclonal anti-mouse IGF-1Rb Ab (Cell Signaling Technology), rabbit monoclonal anti-mouse IR-b (Cell Signaling Technology), or mouse monoclonal anti-mouse b-actin (Abcam) at 4 ̊C overnight.

Techniques: Western Blot, Control, Phospho-proteomics, Expressing, Real-time Polymerase Chain Reaction

FIGURE 2. mTORC1 pathway is more active in insulin-resistant macrophages. Western blot analysis of p-S6 (Ser235/236) and p–4E-BP1 (Thr37/46) in macrophages from Akt22/2 (A) and from short-term HFD–fed mice (B) compared with control before and after 30 min of stimulation with 100 nM insulin. Evaluation of Akt2 phosphorylation (C) and mTORC1 pathway activation (D) by Western blot analysis in control and Igf1R2/2 macrophages under basal conditions and after 30 min of stimulation with 100 nM insulin. (E) Western blot analysis of Akt phosphorylation in WT (control) and Igf1R2/2 macrophages. (F) Western blot analysis of Akt2 phosphorylation in control, HFD, Igf1R2/2, and Akt22/2 macrophages before and after 30 min of stimulation with 2.6 nM IGF1. (G) Akt1 is activated in insulin-resistant macrophages. Western blot analysis of p-Akt1 (Ser473) for control, HFD, Akt22/2 Igf1R2/2, and Akt12/2 macrophages under basal conditions. All graphs are representative of three to six independent experiments and show mean 6 SD. *p , 0.05, **p , 0.01, ***p , 0.001, insulin or IGF1 stimulation versus no stimulation. #p , 0.05, ##p , 0.01, ###p , 0.001, insulin-resistant versus control macrophages.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Insulin Resistance in Macrophages Alters Their Metabolism and Promotes an M2-Like Phenotype.

doi: 10.4049/jimmunol.1800065

Figure Lengend Snippet: FIGURE 2. mTORC1 pathway is more active in insulin-resistant macrophages. Western blot analysis of p-S6 (Ser235/236) and p–4E-BP1 (Thr37/46) in macrophages from Akt22/2 (A) and from short-term HFD–fed mice (B) compared with control before and after 30 min of stimulation with 100 nM insulin. Evaluation of Akt2 phosphorylation (C) and mTORC1 pathway activation (D) by Western blot analysis in control and Igf1R2/2 macrophages under basal conditions and after 30 min of stimulation with 100 nM insulin. (E) Western blot analysis of Akt phosphorylation in WT (control) and Igf1R2/2 macrophages. (F) Western blot analysis of Akt2 phosphorylation in control, HFD, Igf1R2/2, and Akt22/2 macrophages before and after 30 min of stimulation with 2.6 nM IGF1. (G) Akt1 is activated in insulin-resistant macrophages. Western blot analysis of p-Akt1 (Ser473) for control, HFD, Akt22/2 Igf1R2/2, and Akt12/2 macrophages under basal conditions. All graphs are representative of three to six independent experiments and show mean 6 SD. *p , 0.05, **p , 0.01, ***p , 0.001, insulin or IGF1 stimulation versus no stimulation. #p , 0.05, ##p , 0.01, ###p , 0.001, insulin-resistant versus control macrophages.

Article Snippet: Briefly, after blocking with 5% BSA PBS (pH 7.4) for an hour at room temperature, the membranes were incubated with rabbit polyclonal anti-mouse p-Akt (Ser473) Ab (Cell Signaling Technology), rabbit polyclonal anti-mouse Akt Ab (Cell Signaling Technology), rabbit polyclonal anti-mouse p-Akt2 (Ser474) Ab (Cell Signaling Technology), rabbit polyclonal anti-mouse Akt2 Ab (Cell Signaling Technology), rabbit polyclonal anti-mouse p-S6 (235/236) Ab (Cell Signaling Technology), rabbit polyclonal anti-mouse p–4E-BP1(Thr37/46) Ab (Cell Signaling Technology), rabbit polyclonal anti-mouse inducible NO synthase (iNOS) Ab (Abcam), goat polyclonal anti-mouse arginase 1 (Abcam), goat polyclonal anti-mouse Fizz1 Ab (Abcam), rabbit polyclonal anti-mouse IGF-1Rb Ab (Cell Signaling Technology), rabbit monoclonal anti-mouse IR-b (Cell Signaling Technology), or mouse monoclonal anti-mouse b-actin (Abcam) at 4 ̊C overnight.

Techniques: Western Blot, Control, Phospho-proteomics, Activation Assay

FIGURE 3. Insulin-resistant macrophages show reduced M1 responses. (A) Western blot analysis of p-Akt2 (Ser474) in naive and stimulated with LPS (100 ng/ml) for 6 h, WT (control), and insulin-resistant macrophages. (B and C) Levels of IL-6 and TNF-a secreted in the supernatant of cultures of TEPMs and alveolar macrophages (AMs). (D) mRNA expression levels of IL-12 and iNOS of WT (control) and insulin-resistant macrophages before (nonstimulated) and after 6 h stimulation with LPS. (E) Expression levels of iNOS analyzed by Western blot in control and insulin-resistant macrophages before and after 6 h stimulation with LPS. (F) ROS production was evaluated by flow cytometry for control and insulin-resistant TEPMs 6 h after LPS stimulation. Graphs are representative of three to six independent experiments and show mean 6 SD. Box shows 5th to 95th percentiles, horizontal line represents median, and whiskers represent minimum and maximum. *p , 0.05, **p , 0.01, ***p , 0.001, LPS-stimulated versus nonstimulated macrophages. #p , 0.05, ##p , 0.01, ###p , 0.001, insulin-resistant versus control macrophages. MFI, mean fluorescence intensity of TEPMs.

Journal: Journal of immunology (Baltimore, Md. : 1950)

Article Title: Insulin Resistance in Macrophages Alters Their Metabolism and Promotes an M2-Like Phenotype.

doi: 10.4049/jimmunol.1800065

Figure Lengend Snippet: FIGURE 3. Insulin-resistant macrophages show reduced M1 responses. (A) Western blot analysis of p-Akt2 (Ser474) in naive and stimulated with LPS (100 ng/ml) for 6 h, WT (control), and insulin-resistant macrophages. (B and C) Levels of IL-6 and TNF-a secreted in the supernatant of cultures of TEPMs and alveolar macrophages (AMs). (D) mRNA expression levels of IL-12 and iNOS of WT (control) and insulin-resistant macrophages before (nonstimulated) and after 6 h stimulation with LPS. (E) Expression levels of iNOS analyzed by Western blot in control and insulin-resistant macrophages before and after 6 h stimulation with LPS. (F) ROS production was evaluated by flow cytometry for control and insulin-resistant TEPMs 6 h after LPS stimulation. Graphs are representative of three to six independent experiments and show mean 6 SD. Box shows 5th to 95th percentiles, horizontal line represents median, and whiskers represent minimum and maximum. *p , 0.05, **p , 0.01, ***p , 0.001, LPS-stimulated versus nonstimulated macrophages. #p , 0.05, ##p , 0.01, ###p , 0.001, insulin-resistant versus control macrophages. MFI, mean fluorescence intensity of TEPMs.

Article Snippet: Briefly, after blocking with 5% BSA PBS (pH 7.4) for an hour at room temperature, the membranes were incubated with rabbit polyclonal anti-mouse p-Akt (Ser473) Ab (Cell Signaling Technology), rabbit polyclonal anti-mouse Akt Ab (Cell Signaling Technology), rabbit polyclonal anti-mouse p-Akt2 (Ser474) Ab (Cell Signaling Technology), rabbit polyclonal anti-mouse Akt2 Ab (Cell Signaling Technology), rabbit polyclonal anti-mouse p-S6 (235/236) Ab (Cell Signaling Technology), rabbit polyclonal anti-mouse p–4E-BP1(Thr37/46) Ab (Cell Signaling Technology), rabbit polyclonal anti-mouse inducible NO synthase (iNOS) Ab (Abcam), goat polyclonal anti-mouse arginase 1 (Abcam), goat polyclonal anti-mouse Fizz1 Ab (Abcam), rabbit polyclonal anti-mouse IGF-1Rb Ab (Cell Signaling Technology), rabbit monoclonal anti-mouse IR-b (Cell Signaling Technology), or mouse monoclonal anti-mouse b-actin (Abcam) at 4 ̊C overnight.

Techniques: Western Blot, Control, Expressing, Cytometry