akt1 Search Results


akt1  (MedChemExpress)
93
MedChemExpress akt1
( A ) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. ( B ) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. ( C ) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (** P < 0.05). ( D ) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. ( E ) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. ( F ) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. ( G ) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. ( H ) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis ( n = 3 mice per group, **** P < 0.05). ( I ) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis ( n = 3 mice per group). ( J ) NIR images and signal quantification of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. ( K ) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal difference between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
Akt1, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human akt1 catalytic domain  (Carna Inc)
96
Carna Inc human akt1 catalytic domain
( A ) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. ( B ) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. ( C ) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (** P < 0.05). ( D ) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. ( E ) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. ( F ) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. ( G ) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. ( H ) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis ( n = 3 mice per group, **** P < 0.05). ( I ) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis ( n = 3 mice per group). ( J ) NIR images and signal quantification of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. ( K ) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal difference between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
Human Akt1 Catalytic Domain, supplied by Carna Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wild type akt1  (Addgene inc)
90
Addgene inc wild type akt1
( A ) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. ( B ) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. ( C ) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (** P < 0.05). ( D ) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. ( E ) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. ( F ) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. ( G ) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. ( H ) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis ( n = 3 mice per group, **** P < 0.05). ( I ) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis ( n = 3 mice per group). ( J ) NIR images and signal quantification of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. ( K ) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal difference between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
Wild Type Akt1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti akt1 2  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti akt1 2
( A ) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. ( B ) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. ( C ) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (** P < 0.05). ( D ) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. ( E ) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. ( F ) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. ( G ) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. ( H ) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis ( n = 3 mice per group, **** P < 0.05). ( I ) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis ( n = 3 mice per group). ( J ) NIR images and signal quantification of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. ( K ) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal difference between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
Anti Akt1 2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plncxømyr ha akt1 9005  (Addgene inc)
93
Addgene inc plncxømyr ha akt1 9005
( A ) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. ( B ) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. ( C ) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (** P < 0.05). ( D ) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. ( E ) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. ( F ) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. ( G ) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. ( H ) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis ( n = 3 mice per group, **** P < 0.05). ( I ) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis ( n = 3 mice per group). ( J ) NIR images and signal quantification of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. ( K ) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal difference between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
Plncxømyr Ha Akt1 9005, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/plncxømyr ha akt1 9005/product/Addgene inc
Average 93 stars, based on 1 article reviews
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pbabe puro myr flag human akt1  (Addgene inc)
94
Addgene inc pbabe puro myr flag human akt1
( A ) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. ( B ) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. ( C ) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (** P < 0.05). ( D ) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. ( E ) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. ( F ) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. ( G ) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. ( H ) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis ( n = 3 mice per group, **** P < 0.05). ( I ) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis ( n = 3 mice per group). ( J ) NIR images and signal quantification of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. ( K ) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal difference between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
Pbabe Puro Myr Flag Human Akt1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pathscan phospho akt1 sandwich elisa kit  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc pathscan phospho akt1 sandwich elisa kit
( A ) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. ( B ) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. ( C ) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (** P < 0.05). ( D ) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. ( E ) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. ( F ) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. ( G ) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. ( H ) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis ( n = 3 mice per group, **** P < 0.05). ( I ) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis ( n = 3 mice per group). ( J ) NIR images and signal quantification of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. ( K ) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal difference between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
Pathscan Phospho Akt1 Sandwich Elisa Kit, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pathscan phospho akt1 sandwich elisa kit/product/Cell Signaling Technology Inc
Average 94 stars, based on 1 article reviews
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p akt1 2 3 ser 473 r polyclonal antibody  (Santa Cruz Biotechnology)
97
Santa Cruz Biotechnology p akt1 2 3 ser 473 r polyclonal antibody
( A ) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. ( B ) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. ( C ) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (** P < 0.05). ( D ) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. ( E ) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. ( F ) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. ( G ) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. ( H ) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis ( n = 3 mice per group, **** P < 0.05). ( I ) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis ( n = 3 mice per group). ( J ) NIR images and signal quantification of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. ( K ) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal difference between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
P Akt1 2 3 Ser 473 R Polyclonal Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p akt1 2 3 ser 473 r polyclonal antibody/product/Santa Cruz Biotechnology
Average 97 stars, based on 1 article reviews
p akt1 2 3 ser 473 r polyclonal antibody - by Bioz Stars, 2026-02
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mouse monoclonal anti akt1  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc mouse monoclonal anti akt1
( A ) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. ( B ) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. ( C ) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (** P < 0.05). ( D ) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. ( E ) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. ( F ) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. ( G ) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. ( H ) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis ( n = 3 mice per group, **** P < 0.05). ( I ) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis ( n = 3 mice per group). ( J ) NIR images and signal quantification of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. ( K ) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal difference between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
Mouse Monoclonal Anti Akt1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse monoclonal anti akt1 - by Bioz Stars, 2026-02
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anti akt  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti akt
( A ) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. ( B ) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. ( C ) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (** P < 0.05). ( D ) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. ( E ) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. ( F ) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. ( G ) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. ( H ) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis ( n = 3 mice per group, **** P < 0.05). ( I ) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis ( n = 3 mice per group). ( J ) NIR images and signal quantification of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. ( K ) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal difference between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
Anti Akt, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti akt/product/Santa Cruz Biotechnology
Average 96 stars, based on 1 article reviews
anti akt - by Bioz Stars, 2026-02
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akt1 2 sirna  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology akt1 2 sirna
( A ) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. ( B ) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. ( C ) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (** P < 0.05). ( D ) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. ( E ) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. ( F ) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. ( G ) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. ( H ) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis ( n = 3 mice per group, **** P < 0.05). ( I ) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis ( n = 3 mice per group). ( J ) NIR images and signal quantification of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. ( K ) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal difference between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
Akt1 2 Sirna, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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phospho akt  (Cell Signaling Technology Inc)
96
Cell Signaling Technology Inc phospho akt
( A ) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. ( B ) Western blotting for detecting overexpressed albumin and <t>AKT1</t> in the 4T1 tumor sample. ( C ) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (** P < 0.05). ( D ) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. ( E ) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. ( F ) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. ( G ) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. ( H ) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis ( n = 3 mice per group, **** P < 0.05). ( I ) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis ( n = 3 mice per group). ( J ) NIR images and signal quantification of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. ( K ) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal difference between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.
Phospho Akt, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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Image Search Results


( A ) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. ( B ) Western blotting for detecting overexpressed albumin and AKT1 in the 4T1 tumor sample. ( C ) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (** P < 0.05). ( D ) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. ( E ) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. ( F ) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. ( G ) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. ( H ) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis ( n = 3 mice per group, **** P < 0.05). ( I ) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis ( n = 3 mice per group). ( J ) NIR images and signal quantification of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. ( K ) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal difference between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.

Journal: Science Advances

Article Title: Three-dimensional histological electrophoresis enables fast automatic distinguishment of cancer margins and lymph node metastases

doi: 10.1126/sciadv.adg2690

Figure Lengend Snippet: ( A ) Fluorescence images demonstrated the differences between the IR-780–labeled proteins in 4T1 tumor, breast, and muscle lysates following the separation using the 2D SDS-PAGE. ( B ) Western blotting for detecting overexpressed albumin and AKT1 in the 4T1 tumor sample. ( C ) Fraction 1 was selected to distinguish tumor and muscle tissues. Quantification of the Fraction 1–to–Fraction 2 (actin) ratio as annotated in (A), albumin-to-actin ratio, and AKT1-to-actin ratio as annotated in (A) (** P < 0.05). ( D ) IHC staining of albumin and AKT1 levels in 4T1 tumor and muscle tissues. Scale bar, 50 μm. ( E ) Western blotting for detecting albumin and AKT1 levels in a set of 4T1 tumor samples collected after 3, 7, 21, and 35 days of inoculation, respectively. ( F ) Schematic representation of the workflow of the 3D histological electrophoresis for tissue sections. ( G ) Two samples with either muscle&muscle or 4T1 tumor&muscle combination were subjected to the 3D histological electrophoresis. ( H ) The tumor-to-muscle ratios were analyzed and plotted before/after the separation by 3D histological electrophoresis ( n = 3 mice per group, **** P < 0.05). ( I ) By collecting a set of 4T1 tumor samples after 3, 7, 21, and 35 days of inoculation, the tumor-to-muscle ratios were analyzed and plotted after the 3D histological electrophoresis ( n = 3 mice per group). ( J ) NIR images and signal quantification of the seven fractionated layers for the muscle&muscle sample after the 3D histological electrophoresis. Scale bar, 1 cm. ( K ) NIR images and signal quantification of seven fractionated layers for the tumor&muscle sample following the 3D histological electrophoresis. Layers 2, 3, and 4 were observed with the maximum signal difference between tumors and muscles, primed for assessing the tumor-positive margins. Scale bar, 1 cm.

Article Snippet: AKT1 was purchased from MedChemExpress.

Techniques: Fluorescence, Labeling, SDS Page, Western Blot, Immunohistochemistry, Electrophoresis, Muscles