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Image Search Results
Journal: bioRxiv
Article Title: Glycocalyx-Mediated Vascular Dissemination of Circulating Tumor Cells
doi: 10.1101/2020.04.28.066746
Figure Lengend Snippet: Characterization of TC arrest under flow in the MVNs. (a) Schematic diagram of the microfluidic device used to perfuse MVNs under a constant pressure difference Δ p and cells being carried by luminal flow into (A) narrow channels and (B) impacting the endothelium at bifurcations or (C) large vessels (partially realized with Biorender.com). (b) Speed of inert beads carried by luminal flow driven by different pressure differences ( n = 50 beads tracked in 3 devices, average and standard deviation shown) and arrest efficiency of TCs ( n = 4 devices, average and standard deviation shown) as a function of the pressure difference. (c) Confocal image of two arrest mechanisms showing MDA-MB-231 cells within MVNs arrested by (1) physical trapping and (2) adhesion. The scale bar is 200 µm. (d) Bead speed through the MVNs (n as above, the error bars indicate the standard deviation) and vessel diameter as a function of specific GCX component removal (the error bars indicate the standard deviation between the averages of n = 3 devices, 3 regions of interest each). (e) Arrest efficiency of TCs as a function of GCX enzymatic treatment of the MVNs alone, TCs alone, or both MVNs and TCs ( n = 4 devices). (f) Cumulative percentage of TCs either physically trapped in small vessels or adhered to large vessels in the MVNs ( n > 40 cells). Statistical significance assessed for all data portrayed by 138 student’s t-test, p < 0.05 *, p < 0.01 **, p < 0.0001 ****.
Article Snippet: Extravasation experiments were conducted in MVNs cultured within
Techniques: Standard Deviation