aid Search Results


vwr extra pure  (Chem Impex International)
94
Chem Impex International vwr extra pure
Vwr Extra Pure, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcdna5 frt  (Addgene inc)
92
Addgene inc pcdna5 frt
Pcdna5 Frt, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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amyloid beta  (Proteintech)
94
Proteintech amyloid beta
Amyloid Beta, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
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aid  (Cell Signaling Technology Inc)
94
Cell Signaling Technology Inc aid
<t>Immunofluorescence</t> of (A) retinaldehyde dehydrogenase 2 (RALDH2), p75 neurotrophin receptor (p75NTR), and PDGFRβ; (B) β3-tubulin and RALDH2; (C and D) p75NTR; (E) p75NTR and CD21; (F) CD21 and CXCL13; and (G) CD3ε, CD21, and B220 in aged kidneys after ischemic reperfusion injury (IRI). Arrows indicate TLT localization. Arrowheads indicate the localization of the B cell area. Aged kidneys were analyzed 14 (B and C), 30 (A), and 45 (D–G) days after IRI. (H) Retinoic acid (RA) induces p75ntr mRNA expression in C3H10T1/2 mouse embryonic fibroblasts and FACS-sorted PDGFRβ+ cells (n = 3 per group). The data are presented as dot plots (mean ± SD). (I) Activation-induced cytidine deaminase <t>(Aid)</t> mRNA levels of kidneys 45 days after various ischemic time IRI in young and aged mice (n = 4 per group) and correlation with TLT size (n = 16, aged mice only). The expression levels were normalized to those of Gapdh and expressed relative to those of controls or young mouse kidney at day 0 (IRI). *P < 0.001 versus controls. A 2-tailed Student’s t test was used to analyze data from FACS-sorted PDGFRβ+ cells; 1-way ANOVA with Tukey’s post-hoc analysis was used for other experiments. Correlation was determined by Pearson’s correlation analysis. The box corresponds to the first quartile, median (horizontal bar in the box), and third quartile, and the whiskers extend from minimum to maximum values. Scale bars: (A, C, and D) 100 μm, (E and G) 50 μm, (B and F) 10 μm.
Aid, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/aid/product/Cell Signaling Technology Inc
Average 94 stars, based on 1 article reviews
aid - by Bioz Stars, 2026-03
94/100 stars
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anti aid mouse mab  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc anti aid mouse mab
<t>Immunofluorescence</t> of (A) retinaldehyde dehydrogenase 2 (RALDH2), p75 neurotrophin receptor (p75NTR), and PDGFRβ; (B) β3-tubulin and RALDH2; (C and D) p75NTR; (E) p75NTR and CD21; (F) CD21 and CXCL13; and (G) CD3ε, CD21, and B220 in aged kidneys after ischemic reperfusion injury (IRI). Arrows indicate TLT localization. Arrowheads indicate the localization of the B cell area. Aged kidneys were analyzed 14 (B and C), 30 (A), and 45 (D–G) days after IRI. (H) Retinoic acid (RA) induces p75ntr mRNA expression in C3H10T1/2 mouse embryonic fibroblasts and FACS-sorted PDGFRβ+ cells (n = 3 per group). The data are presented as dot plots (mean ± SD). (I) Activation-induced cytidine deaminase <t>(Aid)</t> mRNA levels of kidneys 45 days after various ischemic time IRI in young and aged mice (n = 4 per group) and correlation with TLT size (n = 16, aged mice only). The expression levels were normalized to those of Gapdh and expressed relative to those of controls or young mouse kidney at day 0 (IRI). *P < 0.001 versus controls. A 2-tailed Student’s t test was used to analyze data from FACS-sorted PDGFRβ+ cells; 1-way ANOVA with Tukey’s post-hoc analysis was used for other experiments. Correlation was determined by Pearson’s correlation analysis. The box corresponds to the first quartile, median (horizontal bar in the box), and third quartile, and the whiskers extend from minimum to maximum values. Scale bars: (A, C, and D) 100 μm, (E and G) 50 μm, (B and F) 10 μm.
Anti Aid Mouse Mab, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti aid mouse mab/product/Cell Signaling Technology Inc
Average 93 stars, based on 1 article reviews
anti aid mouse mab - by Bioz Stars, 2026-03
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sodium chloride  (Chem Impex International)
95
Chem Impex International sodium chloride
<t>Immunofluorescence</t> of (A) retinaldehyde dehydrogenase 2 (RALDH2), p75 neurotrophin receptor (p75NTR), and PDGFRβ; (B) β3-tubulin and RALDH2; (C and D) p75NTR; (E) p75NTR and CD21; (F) CD21 and CXCL13; and (G) CD3ε, CD21, and B220 in aged kidneys after ischemic reperfusion injury (IRI). Arrows indicate TLT localization. Arrowheads indicate the localization of the B cell area. Aged kidneys were analyzed 14 (B and C), 30 (A), and 45 (D–G) days after IRI. (H) Retinoic acid (RA) induces p75ntr mRNA expression in C3H10T1/2 mouse embryonic fibroblasts and FACS-sorted PDGFRβ+ cells (n = 3 per group). The data are presented as dot plots (mean ± SD). (I) Activation-induced cytidine deaminase <t>(Aid)</t> mRNA levels of kidneys 45 days after various ischemic time IRI in young and aged mice (n = 4 per group) and correlation with TLT size (n = 16, aged mice only). The expression levels were normalized to those of Gapdh and expressed relative to those of controls or young mouse kidney at day 0 (IRI). *P < 0.001 versus controls. A 2-tailed Student’s t test was used to analyze data from FACS-sorted PDGFRβ+ cells; 1-way ANOVA with Tukey’s post-hoc analysis was used for other experiments. Correlation was determined by Pearson’s correlation analysis. The box corresponds to the first quartile, median (horizontal bar in the box), and third quartile, and the whiskers extend from minimum to maximum values. Scale bars: (A, C, and D) 100 μm, (E and G) 50 μm, (B and F) 10 μm.
Sodium Chloride, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/sodium chloride/product/Chem Impex International
Average 95 stars, based on 1 article reviews
sodium chloride - by Bioz Stars, 2026-03
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glycerol chem impex  (Chem Impex International)
95
Chem Impex International glycerol chem impex
<t>Immunofluorescence</t> of (A) retinaldehyde dehydrogenase 2 (RALDH2), p75 neurotrophin receptor (p75NTR), and PDGFRβ; (B) β3-tubulin and RALDH2; (C and D) p75NTR; (E) p75NTR and CD21; (F) CD21 and CXCL13; and (G) CD3ε, CD21, and B220 in aged kidneys after ischemic reperfusion injury (IRI). Arrows indicate TLT localization. Arrowheads indicate the localization of the B cell area. Aged kidneys were analyzed 14 (B and C), 30 (A), and 45 (D–G) days after IRI. (H) Retinoic acid (RA) induces p75ntr mRNA expression in C3H10T1/2 mouse embryonic fibroblasts and FACS-sorted PDGFRβ+ cells (n = 3 per group). The data are presented as dot plots (mean ± SD). (I) Activation-induced cytidine deaminase <t>(Aid)</t> mRNA levels of kidneys 45 days after various ischemic time IRI in young and aged mice (n = 4 per group) and correlation with TLT size (n = 16, aged mice only). The expression levels were normalized to those of Gapdh and expressed relative to those of controls or young mouse kidney at day 0 (IRI). *P < 0.001 versus controls. A 2-tailed Student’s t test was used to analyze data from FACS-sorted PDGFRβ+ cells; 1-way ANOVA with Tukey’s post-hoc analysis was used for other experiments. Correlation was determined by Pearson’s correlation analysis. The box corresponds to the first quartile, median (horizontal bar in the box), and third quartile, and the whiskers extend from minimum to maximum values. Scale bars: (A, C, and D) 100 μm, (E and G) 50 μm, (B and F) 10 μm.
Glycerol Chem Impex, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/glycerol chem impex/product/Chem Impex International
Average 95 stars, based on 1 article reviews
glycerol chem impex - by Bioz Stars, 2026-03
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anti aβ 1 42  (Proteintech)
95
Proteintech anti aβ 1 42
<t>Immunofluorescence</t> of (A) retinaldehyde dehydrogenase 2 (RALDH2), p75 neurotrophin receptor (p75NTR), and PDGFRβ; (B) β3-tubulin and RALDH2; (C and D) p75NTR; (E) p75NTR and CD21; (F) CD21 and CXCL13; and (G) CD3ε, CD21, and B220 in aged kidneys after ischemic reperfusion injury (IRI). Arrows indicate TLT localization. Arrowheads indicate the localization of the B cell area. Aged kidneys were analyzed 14 (B and C), 30 (A), and 45 (D–G) days after IRI. (H) Retinoic acid (RA) induces p75ntr mRNA expression in C3H10T1/2 mouse embryonic fibroblasts and FACS-sorted PDGFRβ+ cells (n = 3 per group). The data are presented as dot plots (mean ± SD). (I) Activation-induced cytidine deaminase <t>(Aid)</t> mRNA levels of kidneys 45 days after various ischemic time IRI in young and aged mice (n = 4 per group) and correlation with TLT size (n = 16, aged mice only). The expression levels were normalized to those of Gapdh and expressed relative to those of controls or young mouse kidney at day 0 (IRI). *P < 0.001 versus controls. A 2-tailed Student’s t test was used to analyze data from FACS-sorted PDGFRβ+ cells; 1-way ANOVA with Tukey’s post-hoc analysis was used for other experiments. Correlation was determined by Pearson’s correlation analysis. The box corresponds to the first quartile, median (horizontal bar in the box), and third quartile, and the whiskers extend from minimum to maximum values. Scale bars: (A, C, and D) 100 μm, (E and G) 50 μm, (B and F) 10 μm.
Anti Aβ 1 42, supplied by Proteintech, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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pkan aid 9myc  (Addgene inc)
92
Addgene inc pkan aid 9myc
<t>Immunofluorescence</t> of (A) retinaldehyde dehydrogenase 2 (RALDH2), p75 neurotrophin receptor (p75NTR), and PDGFRβ; (B) β3-tubulin and RALDH2; (C and D) p75NTR; (E) p75NTR and CD21; (F) CD21 and CXCL13; and (G) CD3ε, CD21, and B220 in aged kidneys after ischemic reperfusion injury (IRI). Arrows indicate TLT localization. Arrowheads indicate the localization of the B cell area. Aged kidneys were analyzed 14 (B and C), 30 (A), and 45 (D–G) days after IRI. (H) Retinoic acid (RA) induces p75ntr mRNA expression in C3H10T1/2 mouse embryonic fibroblasts and FACS-sorted PDGFRβ+ cells (n = 3 per group). The data are presented as dot plots (mean ± SD). (I) Activation-induced cytidine deaminase <t>(Aid)</t> mRNA levels of kidneys 45 days after various ischemic time IRI in young and aged mice (n = 4 per group) and correlation with TLT size (n = 16, aged mice only). The expression levels were normalized to those of Gapdh and expressed relative to those of controls or young mouse kidney at day 0 (IRI). *P < 0.001 versus controls. A 2-tailed Student’s t test was used to analyze data from FACS-sorted PDGFRβ+ cells; 1-way ANOVA with Tukey’s post-hoc analysis was used for other experiments. Correlation was determined by Pearson’s correlation analysis. The box corresponds to the first quartile, median (horizontal bar in the box), and third quartile, and the whiskers extend from minimum to maximum values. Scale bars: (A, C, and D) 100 μm, (E and G) 50 μm, (B and F) 10 μm.
Pkan Aid 9myc, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
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pcdna3 1 þ vector  (Addgene inc)
92
Addgene inc pcdna3 1 þ vector
<t>Immunofluorescence</t> of (A) retinaldehyde dehydrogenase 2 (RALDH2), p75 neurotrophin receptor (p75NTR), and PDGFRβ; (B) β3-tubulin and RALDH2; (C and D) p75NTR; (E) p75NTR and CD21; (F) CD21 and CXCL13; and (G) CD3ε, CD21, and B220 in aged kidneys after ischemic reperfusion injury (IRI). Arrows indicate TLT localization. Arrowheads indicate the localization of the B cell area. Aged kidneys were analyzed 14 (B and C), 30 (A), and 45 (D–G) days after IRI. (H) Retinoic acid (RA) induces p75ntr mRNA expression in C3H10T1/2 mouse embryonic fibroblasts and FACS-sorted PDGFRβ+ cells (n = 3 per group). The data are presented as dot plots (mean ± SD). (I) Activation-induced cytidine deaminase <t>(Aid)</t> mRNA levels of kidneys 45 days after various ischemic time IRI in young and aged mice (n = 4 per group) and correlation with TLT size (n = 16, aged mice only). The expression levels were normalized to those of Gapdh and expressed relative to those of controls or young mouse kidney at day 0 (IRI). *P < 0.001 versus controls. A 2-tailed Student’s t test was used to analyze data from FACS-sorted PDGFRβ+ cells; 1-way ANOVA with Tukey’s post-hoc analysis was used for other experiments. Correlation was determined by Pearson’s correlation analysis. The box corresponds to the first quartile, median (horizontal bar in the box), and third quartile, and the whiskers extend from minimum to maximum values. Scale bars: (A, C, and D) 100 μm, (E and G) 50 μm, (B and F) 10 μm.
Pcdna3 1 þ Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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plasmid pubc flag  (Addgene inc)
93
Addgene inc plasmid pubc flag
<t>Immunofluorescence</t> of (A) retinaldehyde dehydrogenase 2 (RALDH2), p75 neurotrophin receptor (p75NTR), and PDGFRβ; (B) β3-tubulin and RALDH2; (C and D) p75NTR; (E) p75NTR and CD21; (F) CD21 and CXCL13; and (G) CD3ε, CD21, and B220 in aged kidneys after ischemic reperfusion injury (IRI). Arrows indicate TLT localization. Arrowheads indicate the localization of the B cell area. Aged kidneys were analyzed 14 (B and C), 30 (A), and 45 (D–G) days after IRI. (H) Retinoic acid (RA) induces p75ntr mRNA expression in C3H10T1/2 mouse embryonic fibroblasts and FACS-sorted PDGFRβ+ cells (n = 3 per group). The data are presented as dot plots (mean ± SD). (I) Activation-induced cytidine deaminase <t>(Aid)</t> mRNA levels of kidneys 45 days after various ischemic time IRI in young and aged mice (n = 4 per group) and correlation with TLT size (n = 16, aged mice only). The expression levels were normalized to those of Gapdh and expressed relative to those of controls or young mouse kidney at day 0 (IRI). *P < 0.001 versus controls. A 2-tailed Student’s t test was used to analyze data from FACS-sorted PDGFRβ+ cells; 1-way ANOVA with Tukey’s post-hoc analysis was used for other experiments. Correlation was determined by Pearson’s correlation analysis. The box corresponds to the first quartile, median (horizontal bar in the box), and third quartile, and the whiskers extend from minimum to maximum values. Scale bars: (A, C, and D) 100 μm, (E and G) 50 μm, (B and F) 10 μm.
Plasmid Pubc Flag, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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01523 cas  (Chem Impex International)
95
Chem Impex International 01523 cas
<t>Immunofluorescence</t> of (A) retinaldehyde dehydrogenase 2 (RALDH2), p75 neurotrophin receptor (p75NTR), and PDGFRβ; (B) β3-tubulin and RALDH2; (C and D) p75NTR; (E) p75NTR and CD21; (F) CD21 and CXCL13; and (G) CD3ε, CD21, and B220 in aged kidneys after ischemic reperfusion injury (IRI). Arrows indicate TLT localization. Arrowheads indicate the localization of the B cell area. Aged kidneys were analyzed 14 (B and C), 30 (A), and 45 (D–G) days after IRI. (H) Retinoic acid (RA) induces p75ntr mRNA expression in C3H10T1/2 mouse embryonic fibroblasts and FACS-sorted PDGFRβ+ cells (n = 3 per group). The data are presented as dot plots (mean ± SD). (I) Activation-induced cytidine deaminase <t>(Aid)</t> mRNA levels of kidneys 45 days after various ischemic time IRI in young and aged mice (n = 4 per group) and correlation with TLT size (n = 16, aged mice only). The expression levels were normalized to those of Gapdh and expressed relative to those of controls or young mouse kidney at day 0 (IRI). *P < 0.001 versus controls. A 2-tailed Student’s t test was used to analyze data from FACS-sorted PDGFRβ+ cells; 1-way ANOVA with Tukey’s post-hoc analysis was used for other experiments. Correlation was determined by Pearson’s correlation analysis. The box corresponds to the first quartile, median (horizontal bar in the box), and third quartile, and the whiskers extend from minimum to maximum values. Scale bars: (A, C, and D) 100 μm, (E and G) 50 μm, (B and F) 10 μm.
01523 Cas, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/01523 cas/product/Chem Impex International
Average 95 stars, based on 1 article reviews
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Image Search Results


Immunofluorescence of (A) retinaldehyde dehydrogenase 2 (RALDH2), p75 neurotrophin receptor (p75NTR), and PDGFRβ; (B) β3-tubulin and RALDH2; (C and D) p75NTR; (E) p75NTR and CD21; (F) CD21 and CXCL13; and (G) CD3ε, CD21, and B220 in aged kidneys after ischemic reperfusion injury (IRI). Arrows indicate TLT localization. Arrowheads indicate the localization of the B cell area. Aged kidneys were analyzed 14 (B and C), 30 (A), and 45 (D–G) days after IRI. (H) Retinoic acid (RA) induces p75ntr mRNA expression in C3H10T1/2 mouse embryonic fibroblasts and FACS-sorted PDGFRβ+ cells (n = 3 per group). The data are presented as dot plots (mean ± SD). (I) Activation-induced cytidine deaminase (Aid) mRNA levels of kidneys 45 days after various ischemic time IRI in young and aged mice (n = 4 per group) and correlation with TLT size (n = 16, aged mice only). The expression levels were normalized to those of Gapdh and expressed relative to those of controls or young mouse kidney at day 0 (IRI). *P < 0.001 versus controls. A 2-tailed Student’s t test was used to analyze data from FACS-sorted PDGFRβ+ cells; 1-way ANOVA with Tukey’s post-hoc analysis was used for other experiments. Correlation was determined by Pearson’s correlation analysis. The box corresponds to the first quartile, median (horizontal bar in the box), and third quartile, and the whiskers extend from minimum to maximum values. Scale bars: (A, C, and D) 100 μm, (E and G) 50 μm, (B and F) 10 μm.

Journal: JCI Insight

Article Title: Heterogeneous fibroblasts underlie age-dependent tertiary lymphoid tissues in the kidney

doi: 10.1172/jci.insight.87680

Figure Lengend Snippet: Immunofluorescence of (A) retinaldehyde dehydrogenase 2 (RALDH2), p75 neurotrophin receptor (p75NTR), and PDGFRβ; (B) β3-tubulin and RALDH2; (C and D) p75NTR; (E) p75NTR and CD21; (F) CD21 and CXCL13; and (G) CD3ε, CD21, and B220 in aged kidneys after ischemic reperfusion injury (IRI). Arrows indicate TLT localization. Arrowheads indicate the localization of the B cell area. Aged kidneys were analyzed 14 (B and C), 30 (A), and 45 (D–G) days after IRI. (H) Retinoic acid (RA) induces p75ntr mRNA expression in C3H10T1/2 mouse embryonic fibroblasts and FACS-sorted PDGFRβ+ cells (n = 3 per group). The data are presented as dot plots (mean ± SD). (I) Activation-induced cytidine deaminase (Aid) mRNA levels of kidneys 45 days after various ischemic time IRI in young and aged mice (n = 4 per group) and correlation with TLT size (n = 16, aged mice only). The expression levels were normalized to those of Gapdh and expressed relative to those of controls or young mouse kidney at day 0 (IRI). *P < 0.001 versus controls. A 2-tailed Student’s t test was used to analyze data from FACS-sorted PDGFRβ+ cells; 1-way ANOVA with Tukey’s post-hoc analysis was used for other experiments. Correlation was determined by Pearson’s correlation analysis. The box corresponds to the first quartile, median (horizontal bar in the box), and third quartile, and the whiskers extend from minimum to maximum values. Scale bars: (A, C, and D) 100 μm, (E and G) 50 μm, (B and F) 10 μm.

Article Snippet: The following primary antibodies were used in the mouse immunohistochemistry and immunofluorescence experiments: anti-αSMA (catalog C6198; Sigma-Aldrich), -AID (catalog 4959; Cell Signaling), -GFP (catalog ab13970; Abcam), -Ki67 (catalog 14-5698; eBioscience), -CD45 (catalog 14-0451; eBioscience), –CD11c-PE (catalog 12-0114; eBioscience), –PDGFR-β (catalog 14-1402; eBioscience), –β3tubulin-FITC (catalog A488-435L; Covance), -Cre (catalog 69050-3; Novagen), –LYVE-1 (catalog ab14917; Abcam), -CD21 (catalog ab75985; Abcam), –ER-TR7 (catalog T-2109; BMA Biomedicals), -CXCL13 (catalog AF470; R&D Systems), -CCL19 (catalog AF880; R&D Systems), -CCL21 (catalog AF457; R&D Systems), - p75NTR (catalog AF1157; R&D Systems), -CD3ε (catalog 550275; BD PharMingen), -B220 (catalog 557390; BD PharMingen), –PD-1 (catalog 114102; BioLegend), -CD4 (catalog 100726; BioLegend), -CD8 (catalog 100425; BioLegend), -PNAd (catalog 120801; BioLegend), and -ALDH1A2 (catalog HPA010022; Sigma-Aldrich) antibodies.

Techniques: Immunofluorescence, Expressing, Activation Assay

(A–L) Histological analyses of aged human kidney samples. (A and B) Periodic acid-Schiff (PAS) staining and immunofluorescence analysis of (C) CD20 and CD3ε; (E) Ki67 and CD3ε/CD20 (arrowheads indicate double-positive cells); (G) CXCL13 and CD21; (J) CXCL13 and CD45; (K) CD21, α-smooth muscle actin (αSMA), and CD45; and (L) p75 neurotrophin receptor (p75NTR) and CD21, and immunohistochemical analysis of (D) peripheral lymph node addressin (PNAd); (F) CD21 (arrowheads indicate the localization of CD21+ follicular dendritic cell [FDC] networks); (H) CD20, activation-induced cytidine deaminase (AID), CD21, and retinaldehyde dehydrogenase 2 (RALDH2) (serial sections); and (I) RALDH2 (arrowheads indicate the localization of B cell follicles). The outlined region in (H) is magnified in (I). Arrows indicate TLT localization. Scale bars: (A, D, H, K, and L) 50 μm, (B, C, and F) 100 μm, (E, G, I, and J) 10 μm. (M) Quantitative analysis of TLT frequencies in human samples (n = 56; young = 13, aged = 43). #P < 0.05 (Pearson’s χ2 test).

Journal: JCI Insight

Article Title: Heterogeneous fibroblasts underlie age-dependent tertiary lymphoid tissues in the kidney

doi: 10.1172/jci.insight.87680

Figure Lengend Snippet: (A–L) Histological analyses of aged human kidney samples. (A and B) Periodic acid-Schiff (PAS) staining and immunofluorescence analysis of (C) CD20 and CD3ε; (E) Ki67 and CD3ε/CD20 (arrowheads indicate double-positive cells); (G) CXCL13 and CD21; (J) CXCL13 and CD45; (K) CD21, α-smooth muscle actin (αSMA), and CD45; and (L) p75 neurotrophin receptor (p75NTR) and CD21, and immunohistochemical analysis of (D) peripheral lymph node addressin (PNAd); (F) CD21 (arrowheads indicate the localization of CD21+ follicular dendritic cell [FDC] networks); (H) CD20, activation-induced cytidine deaminase (AID), CD21, and retinaldehyde dehydrogenase 2 (RALDH2) (serial sections); and (I) RALDH2 (arrowheads indicate the localization of B cell follicles). The outlined region in (H) is magnified in (I). Arrows indicate TLT localization. Scale bars: (A, D, H, K, and L) 50 μm, (B, C, and F) 100 μm, (E, G, I, and J) 10 μm. (M) Quantitative analysis of TLT frequencies in human samples (n = 56; young = 13, aged = 43). #P < 0.05 (Pearson’s χ2 test).

Article Snippet: The following primary antibodies were used in the mouse immunohistochemistry and immunofluorescence experiments: anti-αSMA (catalog C6198; Sigma-Aldrich), -AID (catalog 4959; Cell Signaling), -GFP (catalog ab13970; Abcam), -Ki67 (catalog 14-5698; eBioscience), -CD45 (catalog 14-0451; eBioscience), –CD11c-PE (catalog 12-0114; eBioscience), –PDGFR-β (catalog 14-1402; eBioscience), –β3tubulin-FITC (catalog A488-435L; Covance), -Cre (catalog 69050-3; Novagen), –LYVE-1 (catalog ab14917; Abcam), -CD21 (catalog ab75985; Abcam), –ER-TR7 (catalog T-2109; BMA Biomedicals), -CXCL13 (catalog AF470; R&D Systems), -CCL19 (catalog AF880; R&D Systems), -CCL21 (catalog AF457; R&D Systems), - p75NTR (catalog AF1157; R&D Systems), -CD3ε (catalog 550275; BD PharMingen), -B220 (catalog 557390; BD PharMingen), –PD-1 (catalog 114102; BioLegend), -CD4 (catalog 100726; BioLegend), -CD8 (catalog 100425; BioLegend), -PNAd (catalog 120801; BioLegend), and -ALDH1A2 (catalog HPA010022; Sigma-Aldrich) antibodies.

Techniques: Staining, Immunofluorescence, Immunohistochemical staining, Activation Assay