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Image Search Results
Journal: Journal of Biological Chemistry
Article Title: A potential target for liver cancer management, lysophosphatidic acid receptor 6 (LPAR6), is transcriptionally up-regulated by the NCOA3 coactivator
doi: 10.1074/jbc.ra119.009899
Figure Lengend Snippet: Fig. 5 NCOA3 regulates H3K27ac enrichment at LPAR6 locus (A) Schematic diagram of LPAR6 gene and specific primers designed for qPCR. Black box represents CDS region of LPAR6; Gray boxes represent amplification sites by ChIP-qPCR. (B-C) H3K27ac enrichment in (B) HepG2 or (C) Huh7 cells with or without NCOA3 knockdown. Equal amounts of cells were collected after NCOA3 shRNA or scrambled shRNA transfection for 48h and then subject to ChIP. Primers designed for Site 1 amplified LPAR6 coding sequence, while Site 2 and 3 amplified LPAR6 promoter. Error bars represent S.D. from three independent biological replicates. *p< 0.05 and **p< 0.01, significance of difference was analyzed by unpaired t-test. (D) HGF treatment decreased NCOA3 expression in HepG2
Article Snippet:
Techniques: Amplification, ChIP-qPCR, Knockdown, shRNA, Transfection, Sequencing, Expressing