Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Exploring the Mechanism of Edaravone for Oxidative Stress in Rats with Cerebral Infarction Based on Quantitative Proteomics Technology

doi: 10.1155/2022/8653697

Figure Lengend Snippet: Differential expressed protein of CI/CN group.

Article Snippet: RIPA lysate was obtained from Thermo Inc.; iodoacetamide was obtained from IAM, Sigma Aldrich; trypsin was obtained from Promega; ammonium bicarbonate was obtained from Sigma Aldrich Inc.; SOD, MDA, and GSH-px kits were obtained from Shanghai Enzyme Linked Biotechnology Co., Ltd.; rat angiotensinogen (AGT) ELISA kit (E-EL-M0013c) was purchased from Elabscience Inc.

Techniques: Membrane

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Exploring the Mechanism of Edaravone for Oxidative Stress in Rats with Cerebral Infarction Based on Quantitative Proteomics Technology

doi: 10.1155/2022/8653697

Figure Lengend Snippet: Differential expressed protein of CE/CI group.

Article Snippet: RIPA lysate was obtained from Thermo Inc.; iodoacetamide was obtained from IAM, Sigma Aldrich; trypsin was obtained from Promega; ammonium bicarbonate was obtained from Sigma Aldrich Inc.; SOD, MDA, and GSH-px kits were obtained from Shanghai Enzyme Linked Biotechnology Co., Ltd.; rat angiotensinogen (AGT) ELISA kit (E-EL-M0013c) was purchased from Elabscience Inc.

Techniques: Coagulation, Protease Inhibitor

Journal: Molecular Nutrition & Food Research

Article Title: Chlorogenic Acid Ameliorates Chronic Unpredictable Stress‐Induced Diminished Ovarian Reserve Through Ovarian Renin‐Angiotensin System

doi: 10.1002/mnfr.202400814

Figure Lengend Snippet: CGA improved DOR by reducing oxidative stress and apoptosis levels in mouse ovaries through its effects on the ACE‐AngII‐AT1R axis. (A) and (B) The qRT‐PCR analysis of the OVRAS components Ace , Agt , Agtr1a , Agtr1b , Ace2 , Agtr2 , and MasR ( n = 3). (C) Expression levels of oxidative stress and apoptosis molecular markers ( Bax , Bcl‐2 , Sod2 , and Gpx4 ) in the ovaries were detected by qRT‐PCR ( n = 3). (D–F) Quantification of ACE, AGT, AGTR1, ACE2, AGTR2, MasR, BAX, Bcl‐2, SOD2, and GPX4 protein expression. (G) and (H) Photographs of western blot showing the protein level of ACE, ACE2, AGT, AGTR1, AGTR2, MasR, BAX, Bcl‐2, GPX4, and SOD2. (I) and (J) Analysis of AngII and Ang(1‐7) in mouse ovary tissue homogenates collected from each group ( n = 3). CAT (K), GSH (L), and MDA (M) levels as oxidative stress indicators were determined in mouse ovary tissue homogenates ( n = 6). Results are expressed as the mean ± SD and one way ANOVA was used for between‐group comparisons, n represents the number of biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001, ns: not significant.

Article Snippet: Proteins were separated on SDS‐PAGE gels (Epizyme, China), transferred to PVDF membranes (Millipore, USA), and then blocked with 5% non‐fat milk at room temperature for 2 h. The following primary antibodies were used: AGT (Affinity, China, DF7976, 1:1000), AGTR1 (BOSTER, China, BM4949, 1:1000), AGTR2 (BOSTER, China, BM4557, 1:1000), MasR (Affinity, China, DF2818, 1:1000), ACE (Affinity, China, AF5197, 1:1000), ACE2 (Affinity, China, AF5165, 1:1000), BAX (Bimake, USA, A5131, 1:1000), Bcl‐2 (Affinity, China, BF9103, 1:1000), SOD2 (Selleck, USA, A5377, 1:1000), GPX4 (Selleck, USA, A5569, 1:1000), GAPDH (Proteintech, China, 60004‐1‐Ig, 1:10000).

Techniques: Quantitative RT-PCR, Expressing, Western Blot

Journal: Molecular Nutrition & Food Research

Article Title: Chlorogenic Acid Ameliorates Chronic Unpredictable Stress‐Induced Diminished Ovarian Reserve Through Ovarian Renin‐Angiotensin System

doi: 10.1002/mnfr.202400814

Figure Lengend Snippet: CGA decreased activity of ACE‐AngII‐AT1R axis as well as reduced oxidative stress and apoptosis levels of Dex‐induced KGN cells. (A) and (B) The qRT‐PCR analysis of OVRAS components ACE , AGT , AGTR1 , ACE2 , AGTR2 , and MasR mRNA expression level ( n = 3). (C) Expression levels of oxidative stress and apoptosis molecular markers ( BAX , Bcl‐2 , SOD2 , and GPX4 ) detected by qRT‐PCR ( n = 3). (G) and (H) The OVRAS, oxidative stress and apoptosis‐related protein expression in PVDF membrane. (D) and (E) Quantification of ACE, AGT, AGTR1, ACE2, AGTR2, and MasR protein expression ( n = 3). (H) Quantification of BAX, Bcl‐2, SOD2, and GPX4 protein expression ( n = 3). Results are expressed as the mean ± SD and one way ANOVA was used for between‐group comparisons, n represents the number of biological replicates. * p < 0.05, ** p < 0.01, *** p < 0.001, ns: not significant.

Article Snippet: Proteins were separated on SDS‐PAGE gels (Epizyme, China), transferred to PVDF membranes (Millipore, USA), and then blocked with 5% non‐fat milk at room temperature for 2 h. The following primary antibodies were used: AGT (Affinity, China, DF7976, 1:1000), AGTR1 (BOSTER, China, BM4949, 1:1000), AGTR2 (BOSTER, China, BM4557, 1:1000), MasR (Affinity, China, DF2818, 1:1000), ACE (Affinity, China, AF5197, 1:1000), ACE2 (Affinity, China, AF5165, 1:1000), BAX (Bimake, USA, A5131, 1:1000), Bcl‐2 (Affinity, China, BF9103, 1:1000), SOD2 (Selleck, USA, A5377, 1:1000), GPX4 (Selleck, USA, A5569, 1:1000), GAPDH (Proteintech, China, 60004‐1‐Ig, 1:10000).

Techniques: Activity Assay, Quantitative RT-PCR, Expressing, Membrane

Journal: Biology of Sex Differences

Article Title: Sex- and age-related changes in GABA signaling components in the human cortex

doi: 10.1186/s13293-018-0214-6

Figure Lengend Snippet: Primary antibodies used in this study

Article Snippet: Anti-GAT1 , Rabbit, Alomone, AGT-001 , 1:100 , Peptide (C)ERNMHQMTDGLDK.

Techniques: Concentration Assay, Recombinant, Purification