agp-002 Search Results


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Alomone Labs scn2a
(A) Schematic of Na v 1.2 protein showing the position of R853Q and R1882Q mutations (D, Domain; N, N-Terminal; C, C-Terminal). (B) Sanger sequencing of R1882Q and R853Q iPS cell lines and corresponding CRISPR-mediated corrected cell lines (top) maintained normal morphology (bottom). (C) Schematic overview of the NGN2 differentiation protocol with details of crucial molecules exposure and analysis timeline. RT-qPCR analysis of neuronal maturation marker MAP2 and cell identity markers GAD1 and VGLUT at 22 DIV in R1882Q (D) and R853Q (E) iPSC-derived neurons and corresponding control. RT-qPCR analysis of sodium channel genes SCN1A, <t>SCN2A,</t> SCN3A, SCN8A in R1882Q (F) and R853Q (G) iPSC-derived neurons and corresponding control. Data presented as mean ± SD (n=3 experimental biological replicates with n=3 samples per experiment). Unpaired t-test was used to assess statistical significance. Immunofluorescence analyses of MAP2 and SCN2A expression in R1882Q (H) and R853Q (I) iPSC-derived neurons and corresponding control at 22 DIV. Scale bars, (B) 50 μm, (F, G) 20 μm. * p <0.05.
Scn2a, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Alomone Labs antigen p2x4
(A) Schematic of Na v 1.2 protein showing the position of R853Q and R1882Q mutations (D, Domain; N, N-Terminal; C, C-Terminal). (B) Sanger sequencing of R1882Q and R853Q iPS cell lines and corresponding CRISPR-mediated corrected cell lines (top) maintained normal morphology (bottom). (C) Schematic overview of the NGN2 differentiation protocol with details of crucial molecules exposure and analysis timeline. RT-qPCR analysis of neuronal maturation marker MAP2 and cell identity markers GAD1 and VGLUT at 22 DIV in R1882Q (D) and R853Q (E) iPSC-derived neurons and corresponding control. RT-qPCR analysis of sodium channel genes SCN1A, <t>SCN2A,</t> SCN3A, SCN8A in R1882Q (F) and R853Q (G) iPSC-derived neurons and corresponding control. Data presented as mean ± SD (n=3 experimental biological replicates with n=3 samples per experiment). Unpaired t-test was used to assess statistical significance. Immunofluorescence analyses of MAP2 and SCN2A expression in R1882Q (H) and R853Q (I) iPSC-derived neurons and corresponding control at 22 DIV. Scale bars, (B) 50 μm, (F, G) 20 μm. * p <0.05.
Antigen P2x4, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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N/A
The AGPAT5 Antibody (OTI2C4) [DyLight 755] from Novus is a AGPAT5 antibody to AGPAT5. This antibody reacts with Human. The AGPAT5 antibody has been validated for the following applications: Western Blot, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence.
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Lenti ORF clone of Human microfibrillar associated protein 2 MFAP2 transcript variant 2 Myc DDK tagged
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The AGPAT5 Antibody (OTI2C4) [Alexa Fluor® 488] from Novus is a AGPAT5 antibody to AGPAT5. This antibody reacts with Human. The AGPAT5 antibody has been validated for the following applications: Western Blot, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence.
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N/A
The AGPAT5 Antibody (OTI2C4) [DyLight 405] from Novus is a AGPAT5 antibody to AGPAT5. This antibody reacts with Human. The AGPAT5 antibody has been validated for the following applications: Western Blot, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence.
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N/A
The AGPAT5 Antibody (OTI2C4) [DyLight 680] from Novus is a AGPAT5 antibody to AGPAT5. This antibody reacts with Human. The AGPAT5 antibody has been validated for the following applications: Western Blot, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence.
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N/A
The Recombinant Human AGPAT7 Protein has been validated for the following applications Western Blot ELISA Protein Array Immunoaffinity Purification
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Image Search Results


(A) Schematic of Na v 1.2 protein showing the position of R853Q and R1882Q mutations (D, Domain; N, N-Terminal; C, C-Terminal). (B) Sanger sequencing of R1882Q and R853Q iPS cell lines and corresponding CRISPR-mediated corrected cell lines (top) maintained normal morphology (bottom). (C) Schematic overview of the NGN2 differentiation protocol with details of crucial molecules exposure and analysis timeline. RT-qPCR analysis of neuronal maturation marker MAP2 and cell identity markers GAD1 and VGLUT at 22 DIV in R1882Q (D) and R853Q (E) iPSC-derived neurons and corresponding control. RT-qPCR analysis of sodium channel genes SCN1A, SCN2A, SCN3A, SCN8A in R1882Q (F) and R853Q (G) iPSC-derived neurons and corresponding control. Data presented as mean ± SD (n=3 experimental biological replicates with n=3 samples per experiment). Unpaired t-test was used to assess statistical significance. Immunofluorescence analyses of MAP2 and SCN2A expression in R1882Q (H) and R853Q (I) iPSC-derived neurons and corresponding control at 22 DIV. Scale bars, (B) 50 μm, (F, G) 20 μm. * p <0.05.

Journal: bioRxiv

Article Title: Distinctive in vitro phenotypes in iPSC-derived neurons from patients with gain- and loss-of-function SCN2A developmental and epileptic encephalopathy

doi: 10.1101/2023.02.14.528217

Figure Lengend Snippet: (A) Schematic of Na v 1.2 protein showing the position of R853Q and R1882Q mutations (D, Domain; N, N-Terminal; C, C-Terminal). (B) Sanger sequencing of R1882Q and R853Q iPS cell lines and corresponding CRISPR-mediated corrected cell lines (top) maintained normal morphology (bottom). (C) Schematic overview of the NGN2 differentiation protocol with details of crucial molecules exposure and analysis timeline. RT-qPCR analysis of neuronal maturation marker MAP2 and cell identity markers GAD1 and VGLUT at 22 DIV in R1882Q (D) and R853Q (E) iPSC-derived neurons and corresponding control. RT-qPCR analysis of sodium channel genes SCN1A, SCN2A, SCN3A, SCN8A in R1882Q (F) and R853Q (G) iPSC-derived neurons and corresponding control. Data presented as mean ± SD (n=3 experimental biological replicates with n=3 samples per experiment). Unpaired t-test was used to assess statistical significance. Immunofluorescence analyses of MAP2 and SCN2A expression in R1882Q (H) and R853Q (I) iPSC-derived neurons and corresponding control at 22 DIV. Scale bars, (B) 50 μm, (F, G) 20 μm. * p <0.05.

Article Snippet: Immunoreactivity was tested against SCN2A (Na v 1.2) (#AGP-026, Alomone; 1:250), and MAP2 (#ab5622, Abcam; 1:500).

Techniques: Sequencing, CRISPR, Quantitative RT-PCR, Marker, Derivative Assay, Control, Immunofluorescence, Expressing