Journal: Frontiers in Pharmacology

Article Title: Identification and experimental validation of hub genes underlying depressive-like behaviors induced by chronic social defeat stress

doi: 10.3389/fphar.2024.1472468

Figure Lengend Snippet: Primers of AGA, FBXO38, and RGS5 genes.

Article Snippet: The membranes were incubated overnight at 4°C with primary antibodies of RGS5 (1:500, A7015, ABclonal, China), FBXO38 (1:1,000, 83509-6-RR, proteintech, China), AGA (1:2000, 83442-6-RR, proteintech, China) and β-actin (1:80,000, AC026, ABclonal, China) diluted by Western Rapid Kit (L00884, GenScript, China).

Techniques:

Journal: Frontiers in Pharmacology

Article Title: Identification and experimental validation of hub genes underlying depressive-like behaviors induced by chronic social defeat stress

doi: 10.3389/fphar.2024.1472468

Figure Lengend Snippet: Analysis and diagnostic evaluation of hub gene expression. (A–C) Expression of three hub genes (AGA, FBXO38, RGS5) of dlPFC in MDD and control groups. AGA, FBXO38, and RGS5 were significantly reduced in MDD compared with control samples. (D–F) ROC curve for assessing the predictive accuracy of three AGA, FBXO38, and RGS5.

Article Snippet: The membranes were incubated overnight at 4°C with primary antibodies of RGS5 (1:500, A7015, ABclonal, China), FBXO38 (1:1,000, 83509-6-RR, proteintech, China), AGA (1:2000, 83442-6-RR, proteintech, China) and β-actin (1:80,000, AC026, ABclonal, China) diluted by Western Rapid Kit (L00884, GenScript, China).

Techniques: Diagnostic Assay, Gene Expression, Expressing, Control

Journal: Frontiers in Pharmacology

Article Title: Identification and experimental validation of hub genes underlying depressive-like behaviors induced by chronic social defeat stress

doi: 10.3389/fphar.2024.1472468

Figure Lengend Snippet: Performing gene set enrichment analysis (GSEA) analysis of three hub genes. (A, B) High and low expression of top 5 GSEA enrichment in AGA. (C, D) The top 5 GSEA enrichments exhibited both high and low expression levels about FBXO38. (E–F) The top 5 GSEA enrichments showed variable expression levels, including both high and low expression to RGS5.

Article Snippet: The membranes were incubated overnight at 4°C with primary antibodies of RGS5 (1:500, A7015, ABclonal, China), FBXO38 (1:1,000, 83509-6-RR, proteintech, China), AGA (1:2000, 83442-6-RR, proteintech, China) and β-actin (1:80,000, AC026, ABclonal, China) diluted by Western Rapid Kit (L00884, GenScript, China).

Techniques: Expressing

Journal: Frontiers in Pharmacology

Article Title: Identification and experimental validation of hub genes underlying depressive-like behaviors induced by chronic social defeat stress

doi: 10.3389/fphar.2024.1472468

Figure Lengend Snippet: The expression of AGA, FBXO38, and RGS5 decreased after CSDS. (A–C) CSDS reduced the gene expression of AGA (A. n = 7, t (12) = 2.622, p = 0.0223), FBXO38 (B. n = 7, t (12) = 2.184, p = 0.0495), and RGS5 (C. n = 7, t (18) = 2.605, p = 0.023), detected by PCR. (D–F) CSDS reduced the protein expression of AGA (D. n = 8, t (14) = 3.05, p = 0.0087), FBXO38 (E. n = 8, t (14) = 3.19, p = 0.0065), and RGS5 (F. n = 8, t (14) = 2.20, p = 0.0451), detected by Western blot. Student’s t-test. Data expressed as Mean ± SEM. * p < 0.05, ** p < 0.01.

Article Snippet: The membranes were incubated overnight at 4°C with primary antibodies of RGS5 (1:500, A7015, ABclonal, China), FBXO38 (1:1,000, 83509-6-RR, proteintech, China), AGA (1:2000, 83442-6-RR, proteintech, China) and β-actin (1:80,000, AC026, ABclonal, China) diluted by Western Rapid Kit (L00884, GenScript, China).

Techniques: Expressing, Gene Expression, Western Blot