ag18 Search Results


compounds tyrphostin a23  (MedChemExpress)
94
MedChemExpress compounds tyrphostin a23
Compounds Tyrphostin A23, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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egfr inhibitor ag18  (Selleck Chemicals)
85
Selleck Chemicals egfr inhibitor ag18
Figure 3 <t>EGFR</t> is involved in PEDF downregulation. (A and B) Serum-starved LH-15 cells were exposed to an EGFR inhibitor, AG (10 mM) for 30 min. Culture medium was then replaced with fresh 0.1% CS-FBS, supplemented with hCG (1 IU). Cells were incubated for 6 more hours. (C, D, E, and F) Serum-starved (C, D) LH-15 cells or (E and F) PHGC were exposed to AREG (20ng/ml) for 4 or 16 h respectively. (A, C, and E) Changes in PEDF mRNA level were measured by qPCR analysis with specific primers for PEDF (rat or human) and calibrated with HPRT. Bars represent three independent experiments. The ratio between each treatment and control is plotted as MeanGS.E.M., (*P!0.05) – significantly different from control value. (B, D, and F) Cells were lysed and their proteins were analyzed by western blotting with anti-PEDF antibody, using actin as a loading control. Representative blots are presented.
Egfr Inhibitor Ag18, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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uv lamp  (Analytik Jena AG)
94
Analytik Jena AG uv lamp
Figure 3 <t>EGFR</t> is involved in PEDF downregulation. (A and B) Serum-starved LH-15 cells were exposed to an EGFR inhibitor, AG (10 mM) for 30 min. Culture medium was then replaced with fresh 0.1% CS-FBS, supplemented with hCG (1 IU). Cells were incubated for 6 more hours. (C, D, E, and F) Serum-starved (C, D) LH-15 cells or (E and F) PHGC were exposed to AREG (20ng/ml) for 4 or 16 h respectively. (A, C, and E) Changes in PEDF mRNA level were measured by qPCR analysis with specific primers for PEDF (rat or human) and calibrated with HPRT. Bars represent three independent experiments. The ratio between each treatment and control is plotted as MeanGS.E.M., (*P!0.05) – significantly different from control value. (B, D, and F) Cells were lysed and their proteins were analyzed by western blotting with anti-PEDF antibody, using actin as a loading control. Representative blots are presented.
Uv Lamp, supplied by Analytik Jena AG, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-ifn-γ capture antibody ag-18/ra-6a2  (Becton Dickinson)
90
Becton Dickinson anti-ifn-γ capture antibody ag-18/ra-6a2
Figure 3 <t>EGFR</t> is involved in PEDF downregulation. (A and B) Serum-starved LH-15 cells were exposed to an EGFR inhibitor, AG (10 mM) for 30 min. Culture medium was then replaced with fresh 0.1% CS-FBS, supplemented with hCG (1 IU). Cells were incubated for 6 more hours. (C, D, E, and F) Serum-starved (C, D) LH-15 cells or (E and F) PHGC were exposed to AREG (20ng/ml) for 4 or 16 h respectively. (A, C, and E) Changes in PEDF mRNA level were measured by qPCR analysis with specific primers for PEDF (rat or human) and calibrated with HPRT. Bars represent three independent experiments. The ratio between each treatment and control is plotted as MeanGS.E.M., (*P!0.05) – significantly different from control value. (B, D, and F) Cells were lysed and their proteins were analyzed by western blotting with anti-PEDF antibody, using actin as a loading control. Representative blots are presented.
Anti Ifn γ Capture Antibody Ag 18/Ra 6a2, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tyrphostin ag18  (Cayman Chemical)
90
Cayman Chemical tyrphostin ag18
Figure 3 <t>EGFR</t> is involved in PEDF downregulation. (A and B) Serum-starved LH-15 cells were exposed to an EGFR inhibitor, AG (10 mM) for 30 min. Culture medium was then replaced with fresh 0.1% CS-FBS, supplemented with hCG (1 IU). Cells were incubated for 6 more hours. (C, D, E, and F) Serum-starved (C, D) LH-15 cells or (E and F) PHGC were exposed to AREG (20ng/ml) for 4 or 16 h respectively. (A, C, and E) Changes in PEDF mRNA level were measured by qPCR analysis with specific primers for PEDF (rat or human) and calibrated with HPRT. Bars represent three independent experiments. The ratio between each treatment and control is plotted as MeanGS.E.M., (*P!0.05) – significantly different from control value. (B, D, and F) Cells were lysed and their proteins were analyzed by western blotting with anti-PEDF antibody, using actin as a loading control. Representative blots are presented.
Tyrphostin Ag18, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ag18  (ICN Biomedicals)
90
ICN Biomedicals ag18
Figure 3 <t>EGFR</t> is involved in PEDF downregulation. (A and B) Serum-starved LH-15 cells were exposed to an EGFR inhibitor, AG (10 mM) for 30 min. Culture medium was then replaced with fresh 0.1% CS-FBS, supplemented with hCG (1 IU). Cells were incubated for 6 more hours. (C, D, E, and F) Serum-starved (C, D) LH-15 cells or (E and F) PHGC were exposed to AREG (20ng/ml) for 4 or 16 h respectively. (A, C, and E) Changes in PEDF mRNA level were measured by qPCR analysis with specific primers for PEDF (rat or human) and calibrated with HPRT. Bars represent three independent experiments. The ratio between each treatment and control is plotted as MeanGS.E.M., (*P!0.05) – significantly different from control value. (B, D, and F) Cells were lysed and their proteins were analyzed by western blotting with anti-PEDF antibody, using actin as a loading control. Representative blots are presented.
Ag18, supplied by ICN Biomedicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pvp coated ag (18.2 ± 11 nm)  (Promethean Particles Ltd)
90
Promethean Particles Ltd pvp coated ag (18.2 ± 11 nm)
Figure 3 <t>EGFR</t> is involved in PEDF downregulation. (A and B) Serum-starved LH-15 cells were exposed to an EGFR inhibitor, AG (10 mM) for 30 min. Culture medium was then replaced with fresh 0.1% CS-FBS, supplemented with hCG (1 IU). Cells were incubated for 6 more hours. (C, D, E, and F) Serum-starved (C, D) LH-15 cells or (E and F) PHGC were exposed to AREG (20ng/ml) for 4 or 16 h respectively. (A, C, and E) Changes in PEDF mRNA level were measured by qPCR analysis with specific primers for PEDF (rat or human) and calibrated with HPRT. Bars represent three independent experiments. The ratio between each treatment and control is plotted as MeanGS.E.M., (*P!0.05) – significantly different from control value. (B, D, and F) Cells were lysed and their proteins were analyzed by western blotting with anti-PEDF antibody, using actin as a loading control. Representative blots are presented.
Pvp Coated Ag (18.2 ± 11 Nm), supplied by Promethean Particles Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ag18  (Enzo Biochem)
90
Enzo Biochem ag18
Figure 3 <t>EGFR</t> is involved in PEDF downregulation. (A and B) Serum-starved LH-15 cells were exposed to an EGFR inhibitor, AG (10 mM) for 30 min. Culture medium was then replaced with fresh 0.1% CS-FBS, supplemented with hCG (1 IU). Cells were incubated for 6 more hours. (C, D, E, and F) Serum-starved (C, D) LH-15 cells or (E and F) PHGC were exposed to AREG (20ng/ml) for 4 or 16 h respectively. (A, C, and E) Changes in PEDF mRNA level were measured by qPCR analysis with specific primers for PEDF (rat or human) and calibrated with HPRT. Bars represent three independent experiments. The ratio between each treatment and control is plotted as MeanGS.E.M., (*P!0.05) – significantly different from control value. (B, D, and F) Cells were lysed and their proteins were analyzed by western blotting with anti-PEDF antibody, using actin as a loading control. Representative blots are presented.
Ag18, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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uv lamp exposure  (Analytik Jena AG)
86
Analytik Jena AG uv lamp exposure
Figure 3 <t>EGFR</t> is involved in PEDF downregulation. (A and B) Serum-starved LH-15 cells were exposed to an EGFR inhibitor, AG (10 mM) for 30 min. Culture medium was then replaced with fresh 0.1% CS-FBS, supplemented with hCG (1 IU). Cells were incubated for 6 more hours. (C, D, E, and F) Serum-starved (C, D) LH-15 cells or (E and F) PHGC were exposed to AREG (20ng/ml) for 4 or 16 h respectively. (A, C, and E) Changes in PEDF mRNA level were measured by qPCR analysis with specific primers for PEDF (rat or human) and calibrated with HPRT. Bars represent three independent experiments. The ratio between each treatment and control is plotted as MeanGS.E.M., (*P!0.05) – significantly different from control value. (B, D, and F) Cells were lysed and their proteins were analyzed by western blotting with anti-PEDF antibody, using actin as a loading control. Representative blots are presented.
Uv Lamp Exposure, supplied by Analytik Jena AG, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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films with ag-18 thickness  (SAS institute)
90
SAS institute films with ag-18 thickness
Figure 3 <t>EGFR</t> is involved in PEDF downregulation. (A and B) Serum-starved LH-15 cells were exposed to an EGFR inhibitor, AG (10 mM) for 30 min. Culture medium was then replaced with fresh 0.1% CS-FBS, supplemented with hCG (1 IU). Cells were incubated for 6 more hours. (C, D, E, and F) Serum-starved (C, D) LH-15 cells or (E and F) PHGC were exposed to AREG (20ng/ml) for 4 or 16 h respectively. (A, C, and E) Changes in PEDF mRNA level were measured by qPCR analysis with specific primers for PEDF (rat or human) and calibrated with HPRT. Bars represent three independent experiments. The ratio between each treatment and control is plotted as MeanGS.E.M., (*P!0.05) – significantly different from control value. (B, D, and F) Cells were lysed and their proteins were analyzed by western blotting with anti-PEDF antibody, using actin as a loading control. Representative blots are presented.
Films With Ag 18 Thickness, supplied by SAS institute, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 3 EGFR is involved in PEDF downregulation. (A and B) Serum-starved LH-15 cells were exposed to an EGFR inhibitor, AG (10 mM) for 30 min. Culture medium was then replaced with fresh 0.1% CS-FBS, supplemented with hCG (1 IU). Cells were incubated for 6 more hours. (C, D, E, and F) Serum-starved (C, D) LH-15 cells or (E and F) PHGC were exposed to AREG (20ng/ml) for 4 or 16 h respectively. (A, C, and E) Changes in PEDF mRNA level were measured by qPCR analysis with specific primers for PEDF (rat or human) and calibrated with HPRT. Bars represent three independent experiments. The ratio between each treatment and control is plotted as MeanGS.E.M., (*P!0.05) – significantly different from control value. (B, D, and F) Cells were lysed and their proteins were analyzed by western blotting with anti-PEDF antibody, using actin as a loading control. Representative blots are presented.

Journal: REPRODUCTION

Article Title: Pigment epithelium-derived factor regulation by human chorionic gonadotropin in granulosa cells

doi: 10.1530/rep-15-0478

Figure Lengend Snippet: Figure 3 EGFR is involved in PEDF downregulation. (A and B) Serum-starved LH-15 cells were exposed to an EGFR inhibitor, AG (10 mM) for 30 min. Culture medium was then replaced with fresh 0.1% CS-FBS, supplemented with hCG (1 IU). Cells were incubated for 6 more hours. (C, D, E, and F) Serum-starved (C, D) LH-15 cells or (E and F) PHGC were exposed to AREG (20ng/ml) for 4 or 16 h respectively. (A, C, and E) Changes in PEDF mRNA level were measured by qPCR analysis with specific primers for PEDF (rat or human) and calibrated with HPRT. Bars represent three independent experiments. The ratio between each treatment and control is plotted as MeanGS.E.M., (*P!0.05) – significantly different from control value. (B, D, and F) Cells were lysed and their proteins were analyzed by western blotting with anti-PEDF antibody, using actin as a loading control. Representative blots are presented.

Article Snippet: Inhibitors – serum-starved (0.1% CS-FBS) LH-15 cells were incubated for 30 min with i) selective PKC inhibitor GF 109203X (GF, G2911, Sigma); ii) potent cAMP-dependent protein kinase inhibitor H89 dihydrochloride hydrate (H89, B1427, Sigma); iii) MEK (MKK1; MAPK kinase) inhibitor PD184352 (PD, PZ0181 Sigma); iv) phosphatidylinositol 3-kinase (PI3K) inhibitor wortmannin (W1628, Sigma) or v) EGFR inhibitor AG18 (AG; S8009, selleckchem.com, Houston, TX, USA).

Techniques: Incubation, Control, Western Blot