Journal: Cancers

Article Title: Near-Infrared Fluorescent Imaging for Monitoring of Treatment Response in Endometrial Carcinoma Patient-Derived Xenograft Models

doi: 10.3390/cancers12020370

Figure Lengend Snippet: In vitro evaluation of EpCAM-AF680 as a NIRF imaging probe. In vitro imaging of Ishikawa luc+ cells using BLI and EpCAM-AF680 NIRF demonstrates comparable photonic linearity ( A ) and increase in signal ( B ) with higher number of cells (range: 0–10 6 cells). The lack of significant effects of in vitro incubation with EpCAM-AF680 antibody on proliferation and apoptosis demonstrated by MTS assay ( C ) and Annexin V/PI staining ( D ) in various cell lines. Abbreviations: Bioluminescent imaging (BLI), Epithelial cell adhesion molecule (EpCAM), Near-infrared fluorescent (NIRF), and Propium iodide (PI).

Article Snippet: EpCAM-AF680 NIRF images were obtained using the Optix MX3 Time-Domain Optical Imager (ART Inc., Saint-Laurent, QC, Canada), λ ex = 670 nm, λ em = 700 LP, raster scan points 1.5 mm apart.

Techniques: In Vitro, Imaging, Incubation, MTS Assay, Staining

Journal: Cancers

Article Title: Near-Infrared Fluorescent Imaging for Monitoring of Treatment Response in Endometrial Carcinoma Patient-Derived Xenograft Models

doi: 10.3390/cancers12020370

Figure Lengend Snippet: Optical imaging of tumor growth in an orthotopic Ishikawa luc+ xenograft model. In vivo BLI and EpCAM-AF680 NIRF imaging of primary tumor growth in mice orthotopically implanted with Ishikawa luc+ cells. Metastatic lesions (arrows) were detected at an earlier time point in EpCAM-AF680 NIRF images. A tumor free mouse was used as control (upper left) ( A ). Macroscopic images of uterus, pancreas, lungs and abdominal metastases ( B , upper panel) and corresponding ex vivo BLI and EpCAM-AF680 NIRF images ( B , middle panels). Tumor cells are demonstrated in H&E stained sections (20x magnification) ( B , lower panel). Positive EpCAM expression in primary tumor and metastases is demonstrated by IHC ( C ). Abbreviations: Bioluminescent imaging (BLI), Epithelial cell adhesion molecule (EpCAM), Hematoxylin and eosin (H&E), Immunohistochemistry (IHC), and Near-infrared fluorescence (NIRF).

Article Snippet: EpCAM-AF680 NIRF images were obtained using the Optix MX3 Time-Domain Optical Imager (ART Inc., Saint-Laurent, QC, Canada), λ ex = 670 nm, λ em = 700 LP, raster scan points 1.5 mm apart.

Techniques: Optical Imaging, In Vivo, Imaging, Ex Vivo, Staining, Expressing, Immunohistochemistry, Fluorescence

Journal: Cancers

Article Title: Near-Infrared Fluorescent Imaging for Monitoring of Treatment Response in Endometrial Carcinoma Patient-Derived Xenograft Models

doi: 10.3390/cancers12020370

Figure Lengend Snippet: Optical imaging of tumor growth in an orthotopic Hec1B luc+ xenograft model. In vivo BLI and EpCAM-AF680 NIRF imaging of primary tumor growth in mice orthotopically implanted with Hec1B luc+ cells. NIRF imaging enabled detection of metastatic lesions in the lung (arrows), which were not evident on BLI in vivo ( A ). Macroscopic images of organs harvested during necropsy ( B , upper panel) and corresponding ex vivo BLI and EpCAM-AF680 NIRF images ( B , middle panels). Tumor cells are demonstrated in H&E stained sections (20x magnification) ( B , lower panel). Positive EpCAM expression in uterine tumor and metastases is demonstrated by IHC ( C ). Correlation plot of in vivo NIRF and bioluminescent signal in all mice included in the cell line-based models ( D ). Abbreviations: Bioluminescent imaging (BLI), Epithelial cell adhesion molecule (EpCAM), Hematoxylin and eosin (H&E), Immunohistochemistry (IHC), and Near-infrared fluorescence (NIRF).

Article Snippet: EpCAM-AF680 NIRF images were obtained using the Optix MX3 Time-Domain Optical Imager (ART Inc., Saint-Laurent, QC, Canada), λ ex = 670 nm, λ em = 700 LP, raster scan points 1.5 mm apart.

Techniques: Optical Imaging, In Vivo, Imaging, Ex Vivo, Staining, Expressing, Immunohistochemistry, Fluorescence

Journal: Cancers

Article Title: Near-Infrared Fluorescent Imaging for Monitoring of Treatment Response in Endometrial Carcinoma Patient-Derived Xenograft Models

doi: 10.3390/cancers12020370

Figure Lengend Snippet: In vivo imaging of tumor growth in PDX models using EpCAM-AF680 NIRF and 18 F-FDG PET/CT. Longitudinal monitoring of uterine tumors of different histologic types in PDX models using EpCAM-AF680 NIRF and 18 F-FDG PET/CT imaging. Arrows mark probable uterine tumors in PET/CT images ( A – D ). H&E staining demonstrating uterine tumor cells, and positive EpCAM IHC staining of uterine tumors from both donor patients and mouse xenografts (20× magnification) ( E – H ). Large bladder removed from image for visualization purposes. An uncropped version of this image can be found in . Abbreviations: Epithelial cell adhesion molecule (EpCAM), Fluorine-18-fluorodeoxyglucose ( 18 FDG), Hematoxylin and eosin (H&E), Near-infrared fluorescence (NIRF), Patient-derived xenograft (PDX), Positron emission tomography/computed tomography (PET/CT), and Standardized uptake value (SUV).

Article Snippet: EpCAM-AF680 NIRF images were obtained using the Optix MX3 Time-Domain Optical Imager (ART Inc., Saint-Laurent, QC, Canada), λ ex = 670 nm, λ em = 700 LP, raster scan points 1.5 mm apart.

Techniques: In Vivo Imaging, Positron Emission Tomography-Computed Tomography, Imaging, Staining, Immunohistochemistry, Fluorescence, Derivative Assay, Positron Emission Tomography, Computed Tomography

Journal: Cancers

Article Title: Near-Infrared Fluorescent Imaging for Monitoring of Treatment Response in Endometrial Carcinoma Patient-Derived Xenograft Models

doi: 10.3390/cancers12020370

Figure Lengend Snippet: In vivo monitoring of paclitaxel or trastuzumab treatment in an endometrial carcinoma PDX model using EpCAM-AF680 NIRF imaging. EpCAM-AF680 NIRF images of mice treated with control vehicle ( n = 8), paclitaxel ( n = 8) or trastuzumab ( n = 8) prior to (day 26) and after (day 55) treatment. Pre-and post-treatment scans were paired and signal intensity scale synchronized for each individual mouse ( A ). Representative 18 F-FDG PET/CT images (day 47), uterine tumors are indicated by arrows ( B ). Group means of total fluorescent signal before (day 26) and after (day 55) treatment, n = 8 mice per group ( C ). Group means of total fluorescent signal after treatment (day 55, n = 8 mice per group) compared to group SUV mean values measured in 18 F-FDG PET/CT scans at day 47, control group: n = 6, paclitaxel group: n = 5, trastuzumab: n = 6 ( D ). Tumor weights after necropsy on day 49 (control group: mouse 1, paclitaxel group: mouse 2), day 51(control group: mice 3 and 8, trastuzumab group: mouse 2) or day 57 (all other mice), n = 8 in all groups (20× magnification) ( E , F ). Graphs are group means +/− SD. Abbreviations: Epithelial cell adherence molecule (EpCAM), Fluorine-18-fluorodeoxyglucose ( 18 F-FDG), Metabolic tumor volume (MTV), Near infrared fluorescence (NIRF), Positron emission tomography/computed tomography (PET/CT), and Standardized uptake value (SUV).

Article Snippet: EpCAM-AF680 NIRF images were obtained using the Optix MX3 Time-Domain Optical Imager (ART Inc., Saint-Laurent, QC, Canada), λ ex = 670 nm, λ em = 700 LP, raster scan points 1.5 mm apart.

Techniques: In Vivo, Imaging, Positron Emission Tomography-Computed Tomography, Fluorescence, Positron Emission Tomography, Computed Tomography