af647 Search Results


clk 1299 1 thpta jena bioscience  (Jena Bioscience)
99
Jena Bioscience clk 1299 1 thpta jena bioscience
Clk 1299 1 Thpta Jena Bioscience, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dibenzocyclooctyne dbco conjugated to alexafluor647  (Jena Bioscience)
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Jena Bioscience dibenzocyclooctyne dbco conjugated to alexafluor647
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af647  (Lumiprobe)
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Lumiprobe af647
Af647, supplied by Lumiprobe, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alkyne af647 alk 647 jena bioscience cat  (Jena Bioscience)
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cuso4  (Jena Bioscience)
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Jena Bioscience cuso4
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h tet cy5  (Jena Bioscience)
93
Jena Bioscience h tet cy5
( a ) Depiction of the workflow used for expression of ncAA-tagged TARPs in CA1 pyramidal cells in OHSC using single-cell electroporation (SCE), and live staining with tetrazine-dyes. ( b ) Example confocal image of fixed CA1 neurons co-expressing eGFP and γ2 S44* in OHSC. Images are projections of a z-stack taken by 1 μm increments, eGFP signal is color-coded with respect to sample depth. ( c, e ) Representative confocal images of CA1 neurons co-expressing eGFP, and ( c ) γ2 S44*- or ( h ) γ8 S72* live stained with 1 μM <t>H-Tet-Cy5.</t> ( d, f ) Magnified views of segments of the basal and apical dendrites from ( d ) γ2 S44*- and ( f ) γ8 S72*-overexpressing CA1 neurons highlighted in the corresponding overview images (yellow boxes). ( g, i ) Close up of representative spines from ( g ) γ2 S44*- and ( i ) γ8 S72*-overexpressing CA1 neurons highlighted (dashed squares) in the overview images ( d ) and ( f ), respectively. ( h ) and ( j ) Line scan measurements of <t>Cy5</t> signal across spines in ( g ) and ( i ) respectively. ( k, l ) Confocal images of segments of basal dendrites from CA1 neurons co-expressing either ( k ) γ2 S44* or ( l ) γ8 S72, and the PSD-95 marker, XPH20::eGFP. Bottom insets: line scans of the GFP and Cy5 signal for the 3 μm segments indicated in the above images. Scale bar: 100 μm (overview images) and 5 μm (dendritic segments).
H Tet Cy5, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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aminoallyl utp peg5 alexa fluor 647  (Jena Bioscience)
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Jena Bioscience aminoallyl utp peg5 alexa fluor 647
( a ) Depiction of the workflow used for expression of ncAA-tagged TARPs in CA1 pyramidal cells in OHSC using single-cell electroporation (SCE), and live staining with tetrazine-dyes. ( b ) Example confocal image of fixed CA1 neurons co-expressing eGFP and γ2 S44* in OHSC. Images are projections of a z-stack taken by 1 μm increments, eGFP signal is color-coded with respect to sample depth. ( c, e ) Representative confocal images of CA1 neurons co-expressing eGFP, and ( c ) γ2 S44*- or ( h ) γ8 S72* live stained with 1 μM <t>H-Tet-Cy5.</t> ( d, f ) Magnified views of segments of the basal and apical dendrites from ( d ) γ2 S44*- and ( f ) γ8 S72*-overexpressing CA1 neurons highlighted in the corresponding overview images (yellow boxes). ( g, i ) Close up of representative spines from ( g ) γ2 S44*- and ( i ) γ8 S72*-overexpressing CA1 neurons highlighted (dashed squares) in the overview images ( d ) and ( f ), respectively. ( h ) and ( j ) Line scan measurements of <t>Cy5</t> signal across spines in ( g ) and ( i ) respectively. ( k, l ) Confocal images of segments of basal dendrites from CA1 neurons co-expressing either ( k ) γ2 S44* or ( l ) γ8 S72, and the PSD-95 marker, XPH20::eGFP. Bottom insets: line scans of the GFP and Cy5 signal for the 3 μm segments indicated in the above images. Scale bar: 100 μm (overview images) and 5 μm (dendritic segments).
Aminoallyl Utp Peg5 Alexa Fluor 647, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alexa fluor azide b6830  (Lumiprobe)
93
Lumiprobe alexa fluor azide b6830
( a ) Depiction of the workflow used for expression of ncAA-tagged TARPs in CA1 pyramidal cells in OHSC using single-cell electroporation (SCE), and live staining with tetrazine-dyes. ( b ) Example confocal image of fixed CA1 neurons co-expressing eGFP and γ2 S44* in OHSC. Images are projections of a z-stack taken by 1 μm increments, eGFP signal is color-coded with respect to sample depth. ( c, e ) Representative confocal images of CA1 neurons co-expressing eGFP, and ( c ) γ2 S44*- or ( h ) γ8 S72* live stained with 1 μM <t>H-Tet-Cy5.</t> ( d, f ) Magnified views of segments of the basal and apical dendrites from ( d ) γ2 S44*- and ( f ) γ8 S72*-overexpressing CA1 neurons highlighted in the corresponding overview images (yellow boxes). ( g, i ) Close up of representative spines from ( g ) γ2 S44*- and ( i ) γ8 S72*-overexpressing CA1 neurons highlighted (dashed squares) in the overview images ( d ) and ( f ), respectively. ( h ) and ( j ) Line scan measurements of <t>Cy5</t> signal across spines in ( g ) and ( i ) respectively. ( k, l ) Confocal images of segments of basal dendrites from CA1 neurons co-expressing either ( k ) γ2 S44* or ( l ) γ8 S72, and the PSD-95 marker, XPH20::eGFP. Bottom insets: line scans of the GFP and Cy5 signal for the 3 μm segments indicated in the above images. Scale bar: 100 μm (overview images) and 5 μm (dendritic segments).
Alexa Fluor Azide B6830, supplied by Lumiprobe, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5 propargylamino ctp peg5 af647  (Jena Bioscience)
94
Jena Bioscience 5 propargylamino ctp peg5 af647
( a ) Depiction of the workflow used for expression of ncAA-tagged TARPs in CA1 pyramidal cells in OHSC using single-cell electroporation (SCE), and live staining with tetrazine-dyes. ( b ) Example confocal image of fixed CA1 neurons co-expressing eGFP and γ2 S44* in OHSC. Images are projections of a z-stack taken by 1 μm increments, eGFP signal is color-coded with respect to sample depth. ( c, e ) Representative confocal images of CA1 neurons co-expressing eGFP, and ( c ) γ2 S44*- or ( h ) γ8 S72* live stained with 1 μM <t>H-Tet-Cy5.</t> ( d, f ) Magnified views of segments of the basal and apical dendrites from ( d ) γ2 S44*- and ( f ) γ8 S72*-overexpressing CA1 neurons highlighted in the corresponding overview images (yellow boxes). ( g, i ) Close up of representative spines from ( g ) γ2 S44*- and ( i ) γ8 S72*-overexpressing CA1 neurons highlighted (dashed squares) in the overview images ( d ) and ( f ), respectively. ( h ) and ( j ) Line scan measurements of <t>Cy5</t> signal across spines in ( g ) and ( i ) respectively. ( k, l ) Confocal images of segments of basal dendrites from CA1 neurons co-expressing either ( k ) γ2 S44* or ( l ) γ8 S72, and the PSD-95 marker, XPH20::eGFP. Bottom insets: line scans of the GFP and Cy5 signal for the 3 μm segments indicated in the above images. Scale bar: 100 μm (overview images) and 5 μm (dendritic segments).
5 Propargylamino Ctp Peg5 Af647, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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highfidelity af647 pcr labeling kit  (Jena Bioscience)
93
Jena Bioscience highfidelity af647 pcr labeling kit
( a ) Depiction of the workflow used for expression of ncAA-tagged TARPs in CA1 pyramidal cells in OHSC using single-cell electroporation (SCE), and live staining with tetrazine-dyes. ( b ) Example confocal image of fixed CA1 neurons co-expressing eGFP and γ2 S44* in OHSC. Images are projections of a z-stack taken by 1 μm increments, eGFP signal is color-coded with respect to sample depth. ( c, e ) Representative confocal images of CA1 neurons co-expressing eGFP, and ( c ) γ2 S44*- or ( h ) γ8 S72* live stained with 1 μM <t>H-Tet-Cy5.</t> ( d, f ) Magnified views of segments of the basal and apical dendrites from ( d ) γ2 S44*- and ( f ) γ8 S72*-overexpressing CA1 neurons highlighted in the corresponding overview images (yellow boxes). ( g, i ) Close up of representative spines from ( g ) γ2 S44*- and ( i ) γ8 S72*-overexpressing CA1 neurons highlighted (dashed squares) in the overview images ( d ) and ( f ), respectively. ( h ) and ( j ) Line scan measurements of <t>Cy5</t> signal across spines in ( g ) and ( i ) respectively. ( k, l ) Confocal images of segments of basal dendrites from CA1 neurons co-expressing either ( k ) γ2 S44* or ( l ) γ8 S72, and the PSD-95 marker, XPH20::eGFP. Bottom insets: line scans of the GFP and Cy5 signal for the 3 μm segments indicated in the above images. Scale bar: 100 μm (overview images) and 5 μm (dendritic segments).
Highfidelity Af647 Pcr Labeling Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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alexa fluor 647 pcp  (Jena Bioscience)
94
Jena Bioscience alexa fluor 647 pcp
( a ) Depiction of the workflow used for expression of ncAA-tagged TARPs in CA1 pyramidal cells in OHSC using single-cell electroporation (SCE), and live staining with tetrazine-dyes. ( b ) Example confocal image of fixed CA1 neurons co-expressing eGFP and γ2 S44* in OHSC. Images are projections of a z-stack taken by 1 μm increments, eGFP signal is color-coded with respect to sample depth. ( c, e ) Representative confocal images of CA1 neurons co-expressing eGFP, and ( c ) γ2 S44*- or ( h ) γ8 S72* live stained with 1 μM <t>H-Tet-Cy5.</t> ( d, f ) Magnified views of segments of the basal and apical dendrites from ( d ) γ2 S44*- and ( f ) γ8 S72*-overexpressing CA1 neurons highlighted in the corresponding overview images (yellow boxes). ( g, i ) Close up of representative spines from ( g ) γ2 S44*- and ( i ) γ8 S72*-overexpressing CA1 neurons highlighted (dashed squares) in the overview images ( d ) and ( f ), respectively. ( h ) and ( j ) Line scan measurements of <t>Cy5</t> signal across spines in ( g ) and ( i ) respectively. ( k, l ) Confocal images of segments of basal dendrites from CA1 neurons co-expressing either ( k ) γ2 S44* or ( l ) γ8 S72, and the PSD-95 marker, XPH20::eGFP. Bottom insets: line scans of the GFP and Cy5 signal for the 3 μm segments indicated in the above images. Scale bar: 100 μm (overview images) and 5 μm (dendritic segments).
Alexa Fluor 647 Pcp, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat anti human kappa alexa fluor 647 antibody  (SouthernBiotech)
93
SouthernBiotech goat anti human kappa alexa fluor 647 antibody
( a ) Depiction of the workflow used for expression of ncAA-tagged TARPs in CA1 pyramidal cells in OHSC using single-cell electroporation (SCE), and live staining with tetrazine-dyes. ( b ) Example confocal image of fixed CA1 neurons co-expressing eGFP and γ2 S44* in OHSC. Images are projections of a z-stack taken by 1 μm increments, eGFP signal is color-coded with respect to sample depth. ( c, e ) Representative confocal images of CA1 neurons co-expressing eGFP, and ( c ) γ2 S44*- or ( h ) γ8 S72* live stained with 1 μM <t>H-Tet-Cy5.</t> ( d, f ) Magnified views of segments of the basal and apical dendrites from ( d ) γ2 S44*- and ( f ) γ8 S72*-overexpressing CA1 neurons highlighted in the corresponding overview images (yellow boxes). ( g, i ) Close up of representative spines from ( g ) γ2 S44*- and ( i ) γ8 S72*-overexpressing CA1 neurons highlighted (dashed squares) in the overview images ( d ) and ( f ), respectively. ( h ) and ( j ) Line scan measurements of <t>Cy5</t> signal across spines in ( g ) and ( i ) respectively. ( k, l ) Confocal images of segments of basal dendrites from CA1 neurons co-expressing either ( k ) γ2 S44* or ( l ) γ8 S72, and the PSD-95 marker, XPH20::eGFP. Bottom insets: line scans of the GFP and Cy5 signal for the 3 μm segments indicated in the above images. Scale bar: 100 μm (overview images) and 5 μm (dendritic segments).
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Image Search Results


( a ) Depiction of the workflow used for expression of ncAA-tagged TARPs in CA1 pyramidal cells in OHSC using single-cell electroporation (SCE), and live staining with tetrazine-dyes. ( b ) Example confocal image of fixed CA1 neurons co-expressing eGFP and γ2 S44* in OHSC. Images are projections of a z-stack taken by 1 μm increments, eGFP signal is color-coded with respect to sample depth. ( c, e ) Representative confocal images of CA1 neurons co-expressing eGFP, and ( c ) γ2 S44*- or ( h ) γ8 S72* live stained with 1 μM H-Tet-Cy5. ( d, f ) Magnified views of segments of the basal and apical dendrites from ( d ) γ2 S44*- and ( f ) γ8 S72*-overexpressing CA1 neurons highlighted in the corresponding overview images (yellow boxes). ( g, i ) Close up of representative spines from ( g ) γ2 S44*- and ( i ) γ8 S72*-overexpressing CA1 neurons highlighted (dashed squares) in the overview images ( d ) and ( f ), respectively. ( h ) and ( j ) Line scan measurements of Cy5 signal across spines in ( g ) and ( i ) respectively. ( k, l ) Confocal images of segments of basal dendrites from CA1 neurons co-expressing either ( k ) γ2 S44* or ( l ) γ8 S72, and the PSD-95 marker, XPH20::eGFP. Bottom insets: line scans of the GFP and Cy5 signal for the 3 μm segments indicated in the above images. Scale bar: 100 μm (overview images) and 5 μm (dendritic segments).

Journal: bioRxiv

Article Title: Bioorthogonal labeling of transmembrane proteins with non-canonical amino acids allows access to masked epitopes in live neurons

doi: 10.1101/2021.02.27.433189

Figure Lengend Snippet: ( a ) Depiction of the workflow used for expression of ncAA-tagged TARPs in CA1 pyramidal cells in OHSC using single-cell electroporation (SCE), and live staining with tetrazine-dyes. ( b ) Example confocal image of fixed CA1 neurons co-expressing eGFP and γ2 S44* in OHSC. Images are projections of a z-stack taken by 1 μm increments, eGFP signal is color-coded with respect to sample depth. ( c, e ) Representative confocal images of CA1 neurons co-expressing eGFP, and ( c ) γ2 S44*- or ( h ) γ8 S72* live stained with 1 μM H-Tet-Cy5. ( d, f ) Magnified views of segments of the basal and apical dendrites from ( d ) γ2 S44*- and ( f ) γ8 S72*-overexpressing CA1 neurons highlighted in the corresponding overview images (yellow boxes). ( g, i ) Close up of representative spines from ( g ) γ2 S44*- and ( i ) γ8 S72*-overexpressing CA1 neurons highlighted (dashed squares) in the overview images ( d ) and ( f ), respectively. ( h ) and ( j ) Line scan measurements of Cy5 signal across spines in ( g ) and ( i ) respectively. ( k, l ) Confocal images of segments of basal dendrites from CA1 neurons co-expressing either ( k ) γ2 S44* or ( l ) γ8 S72, and the PSD-95 marker, XPH20::eGFP. Bottom insets: line scans of the GFP and Cy5 signal for the 3 μm segments indicated in the above images. Scale bar: 100 μm (overview images) and 5 μm (dendritic segments).

Article Snippet: Pyrimidyl-Tetrazine-Alexa Fluor 647 (Pyr-Tet-AF647; #CLK-102), Pyr-Tet-ATTO-643 (Pyr-Tet-ATTO643; #CLK-101), H-Tet-Cy3 (#CLK-014-05) and H-Tet-Cy5 (#CLK-015-05) were purchased from Jena Bioscience (Jena, Germany).

Techniques: Expressing, Electroporation, Staining, Marker