af519 Search Results


il 10  (R&D Systems)
94
R&D Systems il 10
Il 10, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/il 10/product/R&D Systems
Average 94 stars, based on 1 article reviews
il 10 - by Bioz Stars, 2026-03
94/100 stars
  Buy from Supplier
af519  (R&D Systems)
93
R&D Systems af519
Af519, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/af519/product/R&D Systems
Average 93 stars, based on 1 article reviews
af519 - by Bioz Stars, 2026-03
93/100 stars
  Buy from Supplier
anti-flag antibody  (Beyotime)
90
Beyotime anti-flag antibody
Exogenous expression and localization of ARHGAP31 in mammalian cells. (A) Schematic illustration of molecular structure of <t>FLAG-tagged</t> ARHGAP31. Missense mutation site was shown. (B) Representative immunoblots of exogenous ARHGAP31 expression in HeLa cells using <t>anti-FLAG</t> antibody. (C) Quantification of B ( n = 4 per group). (D) Representative immunofluorescence co-staining of ARHGAP31 expression with anti-FLAG antibody and anti-ARHGAP31 antibody in transfected HeLa cells. Blue, DAPI-labeled nuclei. Arrow denotes ARHGAP31-overexpressing cells and arrowhead denote cells with endogenous ARHGAP31 expression. (E) Colocalization of ARHGAP31 and Golgi marker protein GM130 in transfected HeLa cells. Scale bar, 20 μm.
Anti Flag Antibody, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-flag antibody/product/Beyotime
Average 90 stars, based on 1 article reviews
anti-flag antibody - by Bioz Stars, 2026-03
90/100 stars
  Buy from Supplier

Image Search Results


Exogenous expression and localization of ARHGAP31 in mammalian cells. (A) Schematic illustration of molecular structure of FLAG-tagged ARHGAP31. Missense mutation site was shown. (B) Representative immunoblots of exogenous ARHGAP31 expression in HeLa cells using anti-FLAG antibody. (C) Quantification of B ( n = 4 per group). (D) Representative immunofluorescence co-staining of ARHGAP31 expression with anti-FLAG antibody and anti-ARHGAP31 antibody in transfected HeLa cells. Blue, DAPI-labeled nuclei. Arrow denotes ARHGAP31-overexpressing cells and arrowhead denote cells with endogenous ARHGAP31 expression. (E) Colocalization of ARHGAP31 and Golgi marker protein GM130 in transfected HeLa cells. Scale bar, 20 μm.

Journal: Frontiers in Genetics

Article Title: Synergistic effects of rare variants of ARHGAP31 and FBLN1 in vitro in terminal transverse limb defects

doi: 10.3389/fgene.2022.946854

Figure Lengend Snippet: Exogenous expression and localization of ARHGAP31 in mammalian cells. (A) Schematic illustration of molecular structure of FLAG-tagged ARHGAP31. Missense mutation site was shown. (B) Representative immunoblots of exogenous ARHGAP31 expression in HeLa cells using anti-FLAG antibody. (C) Quantification of B ( n = 4 per group). (D) Representative immunofluorescence co-staining of ARHGAP31 expression with anti-FLAG antibody and anti-ARHGAP31 antibody in transfected HeLa cells. Blue, DAPI-labeled nuclei. Arrow denotes ARHGAP31-overexpressing cells and arrowhead denote cells with endogenous ARHGAP31 expression. (E) Colocalization of ARHGAP31 and Golgi marker protein GM130 in transfected HeLa cells. Scale bar, 20 μm.

Article Snippet: Anti-FLAG antibody (Beyotime biotechnology, AF519, 1: 250 dilution) and anti-HA antibody (Beyotime biotechnology, AF0039, 1:250 dilution) were used.

Techniques: Expressing, Mutagenesis, Western Blot, Immunofluorescence, Staining, Transfection, Labeling, Marker

Increased colocalization with mutant ARHGAP31 and FBLN1. (A) Representative immunofluorescence staining of exogenous ARHGAP31 and FBLN1 in co-transfected HeLa cells. Green, anti-FLAG staining; red, anti-HA staining; and blue, DAPI-labeled nuclei. Arrowheads indicate cells with apparent non-nuclear expression of ARHGAP31, and arrows indicate recognizable cells with co-localized target proteins. (B) HeLa cells were co-transfected with ARHGAP31-FLAG and FBLN1-HA, and co-immunoprecipitation was performed using anti-FLAG antibody followed by detection with anti-HA antibody. (C) ARHGAP31-FLAG and FBLN1-HA co-transfected cells were lyzed, and co-immunoprecipitation was performed using anti-HA antibody followed by immunoblotting with anti-FLAG antibody.

Journal: Frontiers in Genetics

Article Title: Synergistic effects of rare variants of ARHGAP31 and FBLN1 in vitro in terminal transverse limb defects

doi: 10.3389/fgene.2022.946854

Figure Lengend Snippet: Increased colocalization with mutant ARHGAP31 and FBLN1. (A) Representative immunofluorescence staining of exogenous ARHGAP31 and FBLN1 in co-transfected HeLa cells. Green, anti-FLAG staining; red, anti-HA staining; and blue, DAPI-labeled nuclei. Arrowheads indicate cells with apparent non-nuclear expression of ARHGAP31, and arrows indicate recognizable cells with co-localized target proteins. (B) HeLa cells were co-transfected with ARHGAP31-FLAG and FBLN1-HA, and co-immunoprecipitation was performed using anti-FLAG antibody followed by detection with anti-HA antibody. (C) ARHGAP31-FLAG and FBLN1-HA co-transfected cells were lyzed, and co-immunoprecipitation was performed using anti-HA antibody followed by immunoblotting with anti-FLAG antibody.

Article Snippet: Anti-FLAG antibody (Beyotime biotechnology, AF519, 1: 250 dilution) and anti-HA antibody (Beyotime biotechnology, AF0039, 1:250 dilution) were used.

Techniques: Mutagenesis, Immunofluorescence, Staining, Transfection, Labeling, Expressing, Immunoprecipitation, Western Blot