af3138 Search Results


af3138  (R&D Systems Hematology)
93
R&D Systems Hematology af3138
Af3138, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/af3138/product/R&D Systems Hematology
Average 93 stars, based on 1 article reviews
af3138 - by Bioz Stars, 2026-04
93/100 stars
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western blot for gnly  (R&D Systems)
93
R&D Systems western blot for gnly
( a ) SLAY-TCS workflow. ( b ) Sort gate strategy for sort-seq, with sorting bins represented by alternating light and dark grey bars (see Supplementary Table 2 for bin edge fluorescence values). Histogram represents the results from a single biological replicate ( n = 1). ( c ) Calculated fluorescence distributions of no peptide, negative (defensin HNP-1), and positive (LL-37) control strains included in the library. Vertical lines indicate estimated mean fluorescence values for each control. ( d ) Sort-seq fold activation relative to no peptide control strains for all human AMP activators with > 2-fold activation. ( e ) Net charge and hydrophobicity, as measured by grand average of hydropathicity (GRAVY), for all human AMPs included in the screen. Human AMPs belonging to the same cluster ( Supplementary Table 1 ) are represented by a single data point with error bars indicating the median and 25 th to 75 th percentile ranges for that cluster. ( f ) Performance of the cathelicidin sparse robust linear model on peptide variants of human AMP activators of PhoPQ <t>(cathelicidin,</t> <t>CCL20,</t> hBD3, CCL19, NPY, <t>GNLY,</t> TCP) and on peptides variants of non-activating human AMPs (Other). Coefficient of determination ( r 2 ) and Pearson correlation coefficient ( r ) values were calculated for log 10 -transformed fold change and predicted fold change values.
Western Blot For Gnly, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/western blot for gnly/product/R&D Systems
Average 93 stars, based on 1 article reviews
western blot for gnly - by Bioz Stars, 2026-04
93/100 stars
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Image Search Results


( a ) SLAY-TCS workflow. ( b ) Sort gate strategy for sort-seq, with sorting bins represented by alternating light and dark grey bars (see Supplementary Table 2 for bin edge fluorescence values). Histogram represents the results from a single biological replicate ( n = 1). ( c ) Calculated fluorescence distributions of no peptide, negative (defensin HNP-1), and positive (LL-37) control strains included in the library. Vertical lines indicate estimated mean fluorescence values for each control. ( d ) Sort-seq fold activation relative to no peptide control strains for all human AMP activators with > 2-fold activation. ( e ) Net charge and hydrophobicity, as measured by grand average of hydropathicity (GRAVY), for all human AMPs included in the screen. Human AMPs belonging to the same cluster ( Supplementary Table 1 ) are represented by a single data point with error bars indicating the median and 25 th to 75 th percentile ranges for that cluster. ( f ) Performance of the cathelicidin sparse robust linear model on peptide variants of human AMP activators of PhoPQ (cathelicidin, CCL20, hBD3, CCL19, NPY, GNLY, TCP) and on peptides variants of non-activating human AMPs (Other). Coefficient of determination ( r 2 ) and Pearson correlation coefficient ( r ) values were calculated for log 10 -transformed fold change and predicted fold change values.

Journal: bioRxiv

Article Title: High-throughput discovery of peptide activators of a bacterial sensor kinase

doi: 10.1101/2021.06.01.446581

Figure Lengend Snippet: ( a ) SLAY-TCS workflow. ( b ) Sort gate strategy for sort-seq, with sorting bins represented by alternating light and dark grey bars (see Supplementary Table 2 for bin edge fluorescence values). Histogram represents the results from a single biological replicate ( n = 1). ( c ) Calculated fluorescence distributions of no peptide, negative (defensin HNP-1), and positive (LL-37) control strains included in the library. Vertical lines indicate estimated mean fluorescence values for each control. ( d ) Sort-seq fold activation relative to no peptide control strains for all human AMP activators with > 2-fold activation. ( e ) Net charge and hydrophobicity, as measured by grand average of hydropathicity (GRAVY), for all human AMPs included in the screen. Human AMPs belonging to the same cluster ( Supplementary Table 1 ) are represented by a single data point with error bars indicating the median and 25 th to 75 th percentile ranges for that cluster. ( f ) Performance of the cathelicidin sparse robust linear model on peptide variants of human AMP activators of PhoPQ (cathelicidin, CCL20, hBD3, CCL19, NPY, GNLY, TCP) and on peptides variants of non-activating human AMPs (Other). Coefficient of determination ( r 2 ) and Pearson correlation coefficient ( r ) values were calculated for log 10 -transformed fold change and predicted fold change values.

Article Snippet: Macrophage cell lysates were collected at 4 h post-infection and subjected to Western blot for GNLY (R&D Systems Cat# AF3138-SP), CCL20 (R&D Systems Cat# AF360-SP), and/or actin (Abcam Cat# 179467) using 40 μg of total protein.

Techniques: Fluorescence, Control, Activation Assay, Transformation Assay