Journal: Gastroenterology Report

Article Title: TRUB1 is a novel biomarker for promoting malignancy in colorectal cancer via NFκB signaling

doi: 10.1093/gastro/goaf027

Figure Lengend Snippet: Potential downstream targets of TRUB1 in CRC. (A) Scatter plot showing genes with significant changes in RNA-seq after TRUB1 knock-down; (B) GSEA analysis showing enrichment of TNFα signaling via NFκB in cells with high TRUB1 expression; (C) Western blot results for TRUB1, P65, and pP65 in control and TRUB1-knock-down HCT116 cells; (D) intersection analysis of gene sets between HCT116-sh-TRUB1-1 and HCT116-sh-TRUB1-2 (FC ≥ 1.5, FDR ≤ 0.05); (E) intersection analysis of TNFα signaling via NFκB gene sets between HCT116-sh-TRUB1-1 and HCT116-sh-TRUB1-2 ( P ≤ 0.05); (F) intersection analysis of gene sets between GSEA_NFκB signaling (61 genes), RNA-seq (261 genes), and apoptosis via NFκB (33 genes); (G) expression of TRUB1 and BIRC3 mRNA in control and TRUB1-knock-down HCT116 cells. GSEA = Gene Set Enrichment Analysis, pP65 = phosphorylated P65, BIRC3 = Baculoviral IAP repeat-containing protein 3.

Article Snippet: The antibodies that were used in the Western blot were TRUB1 (1:2,000; #PA536003; Thermo, Waltham, MA, USA); TRUB2 (1:2,000; #K110029P; Solarbio, Beijing, China); p65 (1:2,000; #a22331; ABclonal, Woburn, MA, USA); phosphorylated-P65 (pP65; 1:2,000; #af2006; Affinity, Nottingham, UK); GAPDH (1:1,000; #BM3876; BOSTER, Pleasanton, CA, USA); Cytochrome C (1:2,000; #A13430; ABclonal); Bcl 2 (1:2,000; #A0208; ABclonal); Caspase-8 (1:2,000; #A0215; ABclonal); Caspase-3 (1:2,000; #A02156; ABclonal); Caspase-9 (1:2,000; #A11910; ABclonal); cleaved Caspase-3 (1:2,000; #9661 T; CST, Danvers, MA, USA); and β-Actin (1:2,000; #AC038; ABclonal).

Techniques: RNA Sequencing, Knockdown, Expressing, Western Blot, Control

Journal: Frontiers in Nutrition

Article Title: Curcumin attenuates liver injury by modulating the AGE–RAGE axis and metabolic homeostasis in high-fat diet/streptozotocin-induced type 2 diabetic mice

doi: 10.3389/fnut.2025.1710380

Figure Lengend Snippet: CUR modulates AGE/RAGE signaling and its downstream targets PI3K/Akt and NFκB pathways. (A,B) The levels of plasma (A) and hepatic AGEs (B) were measured by ELISA ( n = 5). (C) The mRNA expression levels of RAGE in the liver were analysed ( n = 3). (D–F) Representative bands and quantitative expression levels of p-PI3K, PI3K, p-Akt, Akt, p-NFκB p65, and NFκB p65 proteins in the liver from Western blot were presented. β-actin was served as an internal reference ( n = 3). For quantitative analysis (D–F) , the band intensity of each target protein was normalized to its corresponding internal control. The normalized values were presented relative to the mean of the NC group, which was set to 1. * p < 0.05, ** p < 0.01 and *** p < 0.001; ns, not significant difference.

Article Snippet: Antibodies against PI3K (CAT#AF6241), phospho-PI3K (Tyr 607 ) (CAT#AF3241), Akt (CAT#AF6261), phospho-Akt (Ser 473 ) (CAT#AF0016), phospho-NFκB p65 (Ser 536 ) (CAT#AF2006), TNF- α (CAT#AF7014), and IL-1 β (CAT#AF5103) were purchased from Affinity Biosciences (OH, USA).

Techniques: Clinical Proteomics, Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Control