Journal: Trends in Pharmacological Sciences

Article Title: Ongoing Clinical Trials for the Management of the COVID-19 Pandemic

doi: 10.1016/j.tips.2020.03.006

Figure Lengend Snippet: Key Table. Ongoing Clinical Trials for the (A) Treatment and (B) Prevention of COVID-19 (Current as of 20 March, 2020) a

Article Snippet: NCT04276987 ( ClinicalTrials.gov ) , Arm A: MSC-derived exosomes , 30 , No , No , Not recruiting , China (Cellular Biomedicine Group).

Techniques: Isolation, Injection, Recombinant, Derivative Assay, Purification, Activity Assay

Journal: Advances in Wound Care

Article Title: Human Adipose-Derived Stem Cell Conditioned Media and Exosomes Containing MALAT1 Promote Human Dermal Fibroblast Migration and Ischemic Wound Healing

doi: 10.1089/wound.2017.0775

Figure Lengend Snippet: CM from ADSC promoted cell migration in HDF. HDFs were plated in six-well dishes and when 95% confluent, cells were scratched (3 × ) with a 200 μL pipette. Cells were treated with mitomycin C for 2 h. Cell media from ZenBio™ ADSC were added (2 mL) for 18 h. Unconditioned media (Lonza MSCMCD) were added as the control. After capturing images, migrating cells were counted in a field of the three scratch areas. PRISM™ analysis of data indicated * p < 0.05. ADSC, adipose-derived stem cell; CM, conditioned media; HDF, human dermal fibroblast.

Article Snippet: To probe for secreted factors from ADSC that modulate cell migration, we collected CM from ZenBio subcutaneous derived ADSC.

Techniques: Migration, Transferring, Derivative Assay

Journal: Advances in Wound Care

Article Title: Human Adipose-Derived Stem Cell Conditioned Media and Exosomes Containing MALAT1 Promote Human Dermal Fibroblast Migration and Ischemic Wound Healing

doi: 10.1089/wound.2017.0775

Figure Lengend Snippet: CM from ADSC closed ischemic wounds in Fischer 344 rats. Twenty-four Fisher rats underwent creation of a bipedicled ischemic flap with 6-mm excisional wounds, two ischemic and two nonischemic (control wound). Adult (6 month old) rats were divided into groups of 6, comparing human ADSC-CM obtained from normal donors (ZenBio). Twenty microliters of CM was applied topically daily to each wound beginning on the day of surgery. Digital photographs were taken every 4 days and wound area measured using Image J. Analysis revealed a statistically significant difference between control and lean CM in ischemic wound sizes for days 8, 12, and 16 ( p < 0.001). One hundred percent of lean CM treated ischemic wounds were healed at day 20, 50% of control media.

Article Snippet: To probe for secreted factors from ADSC that modulate cell migration, we collected CM from ZenBio subcutaneous derived ADSC.

Techniques:

Journal: Stem Cells International

Article Title: Adipose-Derived Mesenchymal Stem Cells Migrate and Rescue RPE in the Setting of Oxidative Stress

doi: 10.1155/2018/9682856

Figure Lengend Snippet: Enhanced migration of ASCs following exposure to stressed RPE-CM corresponds to SDF-1 and CXCR4 upregulation in RPE and ASCs, respectively. The migratory ability of ASCs was studied by scratch assay after exposure to stressed RPE-CM (RPE treated with H 2 O 2 ) or to controls comprising ASCs exposed to RPE-CM (RPE cultured without H 2 O 2 ) and non-CM (nonconditioned ADSC medium). (a) ASCs were monitored at 0 and 24 hours postscratch (×10 magnification). (b) Quantification of ASCs' migration by counting invasive cells in scratch boundaries. All scratch assays were performed in quadruplicates, and images were taken at the beginning of the treatments (time zero) and after 24 h (H 2 O 2 treatments). ASCs and RPE cells were harvested and mRNA levels were analyzed using RT-PCR. (c) SDF-1 mRNA in RPE cells incubated with or without H 2 O 2 . (d) CXCR4 mRNA in ASCs incubated with stressed RPE-CM, RPE-CM, or non-CM. CXCR4: chemokine receptor type 4; SDF-1: stromal cell-derived factor 1; RPE: retinal pigment epithelium; ASCs: adipose-derived stem cells; CM: conditioned medium.

Article Snippet: ASCs (1 × 10 6 cells/cm 2 ) at passage 3 or passage 5 were seeded on a 100 mm dish (Falcon) and cultured in ADSC BulletKitTM Medium (Lonza).

Techniques: Migration, Wound Healing Assay, Cell Culture, Reverse Transcription Polymerase Chain Reaction, Incubation, Derivative Assay

Journal: International Journal of Molecular Sciences

Article Title: Exosome Degeneration in Mesenchymal Stem Cells Derived from Patients with Type 1 Diabetes Mellitus

doi: 10.3390/ijms222010906

Figure Lengend Snippet: Exosome particle diameters of adipose-derived mesenchymal stem cells (ADSCs). Results of particle size measurements of ADSC exosomes. ( A ) A graph showing size distribution by intensity measured with a Zetasizer. ( B ) A correlogram generated using a Zetasizer. ( C ) Mean particle diameters of exosomes extracted from normal and adipose-derived stem cells of patients with type 1 diabetes mellitus (T1DM ADSCs). The values shown in the graph are in the form of mean ± SD. The number of samples is n = 5. Three independent evaluations were performed. Shapiro–Wilk and unpaired t -tests were used for statistical analysis. **** The p -value < 0.0001.

Article Snippet: The ADSCs T1DM ADSC (Lonza, Greenwood, SC, USA), The Normal ADSC (Lonza, Greenwood, SC, USA).

Techniques: Derivative Assay, Generated

Journal: International Journal of Molecular Sciences

Article Title: Exosome Degeneration in Mesenchymal Stem Cells Derived from Patients with Type 1 Diabetes Mellitus

doi: 10.3390/ijms222010906

Figure Lengend Snippet: Quantification of exosomes for adipose-derived stem cells of patients with type 1 diabetes mellitus (T1DM ADSC). Results of quantification of exosomes extracted from adipose-derived mesenchymal stem cells (ADSCs) by colorimetry using acetylcholinesterase (AChE) activity as an index. ( A ) A standard curve created with two-fold serial dilutions of an exosome AChE standard. ( B ) A graph showing exosome counts calculated using the standard curve obtained from absorbance values measured at 405 nm by colorimetry; the exosomes were collected from the same volume of normal and T1DM ADSCs culture supernatants. Values shown in the graph are in the form of mean ± SEM. The number of samples is n = 3. Three independent evaluations were performed. Shapiro–Wilk and unpaired t -tests were used for statistical analysis. ** The p -value was 0.0023.

Article Snippet: The ADSCs T1DM ADSC (Lonza, Greenwood, SC, USA), The Normal ADSC (Lonza, Greenwood, SC, USA).

Techniques: Derivative Assay, Colorimetric Assay, Activity Assay