Journal: World Journal of Gastroenterology

Article Title: B2 adrenergic receptors and morphological changes of the enteric nervous system in colorectal adenocarcinoma

doi: 10.3748/wjg.v23.i7.1250

Figure Lengend Snippet: Example of spectral unmixing for the series of slides. A: Immunostained for beta-2 adrenergic receptors with DAB and counterstained with hematoxylin; B: Pure DAB and hematoxylin signals are shown either overlapping or (C and D), individually.

Article Snippet: After we reviewed the slides and confirmed the pathologies and tumor gradings, three μm-thick serial sections were cut from each block, deparaffinized in xylene, rehydrated in graded alcohol series, and subjected to enzymatic immunohistochemistry utilizing a rabbit-anti-human anti-beta-2 adrenergic receptor (NBP2-15564, diluted as 1:200, Novus Biologicals, United Kingdom) and a rabbit anti-human anti-S100 (Z0311, diluted as 1:100, Dako, Glostrup, Denmark) primary antibodies.

Techniques:

Journal: World Journal of Gastroenterology

Article Title: B2 adrenergic receptors and morphological changes of the enteric nervous system in colorectal adenocarcinoma

doi: 10.3748/wjg.v23.i7.1250

Figure Lengend Snippet: Analysis of the expression of beta-2 adrenergic receptors in the normal and tumor colon tissue. RGB (A) and unmixed (B; b1, b1a; b2, b2a) images showing receptor presence mostly as dense granules in the supranuclear sector of the superficial layer of enterocytes (b1; b1a), as well as in the sub-nuclear region of the glandular cells of the crypts (b2; b2a). In well differentiated adenocarcinoma, RGB (C) and unmixed images (D; d1, d1a; d2, d2a) revealed a more dense-diffuse like pattern of expression mostly in the apical sector of the cells (d, d1a), but also in the basal pole of the cells (d2, d2a). In moderately differentiated adenocarcinoma, RGB (E) and unmixed images (F; f1, f1a; f2, f2a) revealed an ever denser and more uniform expression pattern in all the cytoplasm of the tumor cells. In poorly differentiated adenocarcinomas, RGB (G) and unmixed images (H; h1, h1a; h2, h2a) reveal an even denser granular expression pattern, with occasional intra(peri) nuclear localization (arrows in h2 and h2a). Small letters represent enlarged insets from the capital lettered images.

Article Snippet: After we reviewed the slides and confirmed the pathologies and tumor gradings, three μm-thick serial sections were cut from each block, deparaffinized in xylene, rehydrated in graded alcohol series, and subjected to enzymatic immunohistochemistry utilizing a rabbit-anti-human anti-beta-2 adrenergic receptor (NBP2-15564, diluted as 1:200, Novus Biologicals, United Kingdom) and a rabbit anti-human anti-S100 (Z0311, diluted as 1:100, Dako, Glostrup, Denmark) primary antibodies.

Techniques: Expressing

Journal: World Journal of Gastroenterology

Article Title: B2 adrenergic receptors and morphological changes of the enteric nervous system in colorectal adenocarcinoma

doi: 10.3748/wjg.v23.i7.1250

Figure Lengend Snippet: Expression of beta-2 adrenergic receptors in the normal and tumor peripheral nervous tissue on serial sections immunostained for S100 protein (A, C, E, G) and B2A (B, b1; D; d1; F, f1; H, h1). Spectral unmixing showed that in the normal submucosal (b1) and myenteric (d1) plexuses, B2A was mostly expressed in the cytoplasm of the ganglion cells, being absent in small nerves dissecting the muscularis proper layer (C and D). Even in larger non-invaded nerves B2A showed a faint expression (E-F, f1 spectral unmixing). However, in invaded ganglia (G, g1) and nerves (H, h1) its expression seems to be expressed in both the cytoplasm of the ganglion cells (arrows in g1 - unmixed), as well as in the larger nerve bundles (h1 - unmixed). Small letters represent enlarged insets from the capital lettered images.

Article Snippet: After we reviewed the slides and confirmed the pathologies and tumor gradings, three μm-thick serial sections were cut from each block, deparaffinized in xylene, rehydrated in graded alcohol series, and subjected to enzymatic immunohistochemistry utilizing a rabbit-anti-human anti-beta-2 adrenergic receptor (NBP2-15564, diluted as 1:200, Novus Biologicals, United Kingdom) and a rabbit anti-human anti-S100 (Z0311, diluted as 1:100, Dako, Glostrup, Denmark) primary antibodies.

Techniques: Expressing

Journal: World Journal of Gastroenterology

Article Title: B2 adrenergic receptors and morphological changes of the enteric nervous system in colorectal adenocarcinoma

doi: 10.3748/wjg.v23.i7.1250

Figure Lengend Snippet: Analysis of the expression of beta-2 adrenergic receptors. Total expression area increases gradually from normal tissue to G1, G2 and G3 differentiated adenocarcinoma (A), and this differentiation is driven by the expression in the epithelial areas (B), where assessing stromal signal revealed no differences (C). The same scenario is obtained when evaluating the integrated optical density (IOD) of the signal (D-F). a P < 0.05 represents statistical significance on one-way analysis of variance with post hoc comparisons using the Bonferroni’s test. Error bars represent standard errors of the mean. B2A: Beta-2 adrenergic.

Article Snippet: After we reviewed the slides and confirmed the pathologies and tumor gradings, three μm-thick serial sections were cut from each block, deparaffinized in xylene, rehydrated in graded alcohol series, and subjected to enzymatic immunohistochemistry utilizing a rabbit-anti-human anti-beta-2 adrenergic receptor (NBP2-15564, diluted as 1:200, Novus Biologicals, United Kingdom) and a rabbit anti-human anti-S100 (Z0311, diluted as 1:100, Dako, Glostrup, Denmark) primary antibodies.

Techniques: Expressing

Journal: World Journal of Gastroenterology

Article Title: B2 adrenergic receptors and morphological changes of the enteric nervous system in colorectal adenocarcinoma

doi: 10.3748/wjg.v23.i7.1250

Figure Lengend Snippet: Relationship between beta-2 adrenergic receptor expression and clinicopathological features

Article Snippet: After we reviewed the slides and confirmed the pathologies and tumor gradings, three μm-thick serial sections were cut from each block, deparaffinized in xylene, rehydrated in graded alcohol series, and subjected to enzymatic immunohistochemistry utilizing a rabbit-anti-human anti-beta-2 adrenergic receptor (NBP2-15564, diluted as 1:200, Novus Biologicals, United Kingdom) and a rabbit anti-human anti-S100 (Z0311, diluted as 1:100, Dako, Glostrup, Denmark) primary antibodies.

Techniques: Expressing

Journal: Nature Communications

Article Title: An artificial cell capable of signal transduction mediated by ADRB2 for the regulation of glycogenolysis

doi: 10.1038/s41467-026-68503-3

Figure Lengend Snippet: a The signal transduction from extracellular ISO into intracellular cAMP and subsequent regulation of glycogenolytic metabolic pathway inside an artificial cell; ISO: isoproterenol; ADRB2: β2-adrenergic receptor; Gsα: Gs subunit α; ADCY5: adenylate cyclase V; PKA: Protein kinase A; PhK: Phosphorylase kinase; PYGM: Glycogen phosphorylase; PGM: Phosphoglucomutase; G6PDH: Glucose-6-phosphate dehydrogenase; GDP: Guanosine diphosphate; GTP: Guanosine triphosphate; ATP: Adenosine triphosphate; cAMP: Cyclic adenosine monophosphate; G-1-P: Glucose 1-phosphate; G-6-P: Glucose-6-phosphate; 6-PGL: 6-Phosphoglucono-lactone; NADP⁺(H): Nicotinamide adenine dinucleotide phosphate. Created in BioRender. Liu, Y. (2025) https://BioRender.com/689u8j4 . b Chemical reaction equations involved in the signal transduction and glycogenolytic metabolism; ADCY5: Adenylate cyclase V; p-PYGM: Phosphorylated glycogen phosphorylase.

Article Snippet: Rabbit anti-PhKA2 polyclonal antibody (No. 24658-1-AP), rabbit anti-PYGM-Specific polyclonal antibody (No. 19716-1-AP) and ADRB2 polyclonal antibody (No. 29864-1-AP) were purchased from Proteintech (China).

Techniques: Transduction

Journal: Nature Communications

Article Title: An artificial cell capable of signal transduction mediated by ADRB2 for the regulation of glycogenolysis

doi: 10.1038/s41467-026-68503-3

Figure Lengend Snippet: a Scheme of the signal transduction pathway from ISO to cAMP mediated by ADRB2. SDS-PAGE images of purified ADRB2 ( b ), Gsα ( c ) and ADCY5 ( d ) from Homo Sapiens . n = 3 independent replicates. e The size exclusion chromatography of ADRB2, Gsα, and ADRB2-Gsα complex. f The effect of pH on the ADCY5 activity by measuring the production of cAMP at 30 °C, 10.0 μM ISO and 2.0 mM Mg 2+ . g The effect of temperature on the ADCY5 activity by measuring the production of cAMP at pH 8, 10.0 μM ISO and 2.0 mM Mg 2+ . The data were expressed as mean ± SD, n = 3 independent replicates. h The Mg 2+ concentration effect on the ADCY5 activity by measuring the production of cAMP at 37 °C, pH 8 and 10.0 μM ISO. The data were expressed as mean ± SD, n = 3 independent replicates. i Initial reaction velocities of cAMP production catalyzed by ADCY5 as a function of ATP concentration from 0 to 14.0 mM in the solution (10.0 μM ISO, 2.0 mM Mg 2+ , 6.0 μg/mL ADRB2-Gsα complex, 6.0 μg/mL ADCY5, pH 8) at 37 °C. The data were expressed as mean ± SD, n = 3 independent replicates. j The cAMP production as a function of ADRB2-Gsα/ADCY5 molar ratios with ADCY5 fixed at 10 nM and ADRB2-Gsα varied from 1.1 to 21.7 nM in a solution (10.0 μM ISO, 2.0 mM Mg 2+ , pH 8) at 37 °C. The data were expressed as mean ± SD, n = 3 independent replicates. **** P < 0.0001, ns P > 0.05, two-sided, multiple comparisons by One-way ANOVA test. k ISO dose-dependent cAMP production as a function of time in a solution (17.4 nM ADRB2-Gsα, 10.0 nM ADCY5, 10 −5 −10 3 μM ISO, 2.0 mM Mg 2+ , pH 8) at 37 °C. l The corresponding cAMP production at 25 min of ( k ). The data were expressed as mean ± SD, n = 3 independent replicates. **** P < 0.0001, ns P > 0.05, two-sided, multiple comparisons by One-way ANOVA test. P < 0.05 was considered statistically significant. Source data are provided as a Source Data file.

Article Snippet: Rabbit anti-PhKA2 polyclonal antibody (No. 24658-1-AP), rabbit anti-PYGM-Specific polyclonal antibody (No. 19716-1-AP) and ADRB2 polyclonal antibody (No. 29864-1-AP) were purchased from Proteintech (China).

Techniques: Transduction, SDS Page, Purification, Size-exclusion Chromatography, Activity Assay, Concentration Assay

Journal: Nature Communications

Article Title: An artificial cell capable of signal transduction mediated by ADRB2 for the regulation of glycogenolysis

doi: 10.1038/s41467-026-68503-3

Figure Lengend Snippet: a The representative fluorescence images of Cy5-ADRB2-GUV (with NBD-PE in the bilayer) taken with red channel (left image), green channel (middle image), and their merged image (right image) at ADRB2 and lipids molar ratio of 10 3 :10 6 . The scale bar is 10.0 μm. b The percentage of ADRB2-GUVs with varied ADRB2 to lipids molar ratios ranging from 1:10 6 to 10 4 :10 6 . The data were expressed as mean ± SD, n = 3 independent replicates. **** P < 0.0001 1:10 6 vs. 10:10 6 , **** P < 0.0001 10:10 6 vs. 10 2 :10 6 , *** P = 0.0007 10 2 :10 6 vs. 10 3 :10 6 , ns P > 0.05 10 3 :10 6 vs. 10 4 :10 6 , two-sided, multiple comparisons by One-way ANOVA test. c The percentage of ADRB2 with correct orientation on GUVs determined by WB (top image) and band intensities (bottom graph). The data were expressed as mean ± SD, n = 3 independent replicates. d Schematic diagram of the Gsα detection mechanism using BODIPY TR GTP γ S. Created in BioRender. Liu, Y. (2025) https://BioRender.com/g8zgjzy . e The representative time-series images of GUVs stimulated by 1.0 μM ISO for the binding events of BODIPY TR GTP γ S to Gsα from 30 s to 15 min. The scale bar is 10.0 μm. n = 3 independent replicates. f The corresponding fluorescence intensity of GUV in ( e ) (red curve) and the fluorescence intensity of GUV with no ISO stimulation (blue curve). n = 3 independent replicates. g The representative fluorescence images of FITC labeled ADCY5-GUV (with TR-DHPE in the bilayer) taken with green channel (left image), red channel (middle image), and their merged image (right image) at ADCY5 and lipids molar ratio of 10 3 :10 6 . The scale bar is 10.0 μm. n = 3 independent replicates. h The flow cytometry histogram of FITC-ADCY5-GUVs with varied ADCY5 to lipids molar ratios ranging from 10 −1 :10 6 to 10 3 :10 6 . n = 3 independent replicates. i The percentage of ADCY5-GUVs with varied ADCY5 to lipids molar ratios ranging from 10 −1 :10 6 to 10 3 :10 6 . The data were expressed as mean ± SD, n = 3 independent replicates. **** P < 0.0001 10 −1 :10 6 vs. 1:10 6 , **** P < 0.0001 1:10 6 vs. 10:10 6 , **** P < 0.0001 10:10 6 vs. 10 2 :10 6 , **** P < 0.0001 10 2 :10 6 vs. 10 3 :10 6 , two-sided, multiple comparisons by One-way ANOVA test. P < 0.05 was considered statistically significant. Source data are provided as a Source Data file.

Article Snippet: Rabbit anti-PhKA2 polyclonal antibody (No. 24658-1-AP), rabbit anti-PYGM-Specific polyclonal antibody (No. 19716-1-AP) and ADRB2 polyclonal antibody (No. 29864-1-AP) were purchased from Proteintech (China).

Techniques: Fluorescence, Binding Assay, Labeling, Flow Cytometry

Journal: Nature Communications

Article Title: An artificial cell capable of signal transduction mediated by ADRB2 for the regulation of glycogenolysis

doi: 10.1038/s41467-026-68503-3

Figure Lengend Snippet: a Schematic illustration of the signal transduction of an artificial cell from extracellular ISO to intracellular cAMP. Created in BioRender. Liu, Y. (2025) https://BioRender.com/ik5i982 . b Fluorescence images of GUVs containing Cy5-ADRB2-Gsα complexes and FITC-ADCY5 taken by red channel (left image), green channel (middle image), and their merged image (right image). The scale bar is 10.0 μm. n = 3 independent replicates. c The representative time-series images of artificial cells stimulated by 1.0 μM ISO for the intracellular cAMP production from 0 to 20 min, with top row images taken by the blue channel and bottom row images taken by the green channel. The scale bar is 10.0 μm. n = 3 independent replicates. d The corresponding FRET ratios of Epac1-cAMP in the artificial cell in ( c ). The data were expressed as mean ± SD, n = 3 independent replicates. e The cAMP production in artificial cells with the addition of 1.0 μM ISO as a function of time from 0 to 30 min using fluorescence spectroscopy. n = 3 independent replicates. f The cAMP production in artificial cells with the addition of varied ISO concentration (0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 2.0, 4.0, 6.0, 8.0, 10.0 μM) at 30 min. n = 3 independent replicates. g The signal amplification folds of artificial cells from extracellular ISO to intracellular cAMP as a function of ISO concentration (0.4 to 10.0 μM) at 30 min. n = 3 independent replicates. h The representative time-series images of Epac1-cAMP in artificial cells with sequential antagonist alprenolol treatment for 10 min from 10 to 20 min, and competitive 10.0 μM ISO activation for 10 min from 20 to 30 min, with top row images taken by blue channel and bottom row images taken by green channel. The scale bar is 10.0 μm. n = 3 independent replicates. i The corresponding Fret ratio of Epac1-cAMP in the artificial cell in ( h ). The data were expressed as mean ± SD, n = 3 independent replicates. j The representative time-series images of Epac1-cAMP in artificial cells with sequential inverse agonist carazolol treatment for 10 min from 10 to 20 min, and 10.0 μM ISO activation for 10 min from 20 to 30 min, with top row images taken by blue channel and bottom row images taken by green channel. The scale bar is 10.0 μm. n = 3 independent replicates. k The corresponding FRET ratio Epac1-cAMP in the artificial cell in ( j ). The data were expressed as mean ± SD, n = 3 independent replicates. l FRET ratio of Epac1-cAMP in the artificial cell measured by fluorescence spectroscopy as a function of time with the addition of 1.0 μM alprenolol antagonism at 10 min and the addition of 10.0 μM ISO at 20 min (blue curve), with the addition of 1.0 μM carazolol inverse agonism at 10 min and the addition of 10.0 μM ISO at 20 min (green curve). The data were expressed as mean ± SD, n = 3 independent replicates. Source data are provided as a Source Data file.

Article Snippet: Rabbit anti-PhKA2 polyclonal antibody (No. 24658-1-AP), rabbit anti-PYGM-Specific polyclonal antibody (No. 19716-1-AP) and ADRB2 polyclonal antibody (No. 29864-1-AP) were purchased from Proteintech (China).

Techniques: Transduction, Fluorescence, Spectroscopy, Concentration Assay, Amplification, Activation Assay

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Paroxetine mitigates cardiac remodelling by doxorubicin and increases survival.

doi: 10.1016/j.biopha.2021.112411

Figure Lengend Snippet: Fig. 2. Relative mRNA expression of cardiac β1-AR, β2-AR, GRK2, and GRK3 in rats treated with P. Note a statistically significant decrease of GRK2 mRNA levels in rats treated with paroxetine, 10 mg/kg/day p.o. Data represent the mean of five rats ± SEM. *p < 0.05 vs. control group (One-way ANOVA fol lowed by Tukey’s multiple comparisons). β-AR, beta-adrenergic receptor; GRK, G protein-coupled receptor kinase.

Article Snippet: Immunohistochemistry was performed manually on the 5 μm-thick sections, according to the manufacturer’s instructions using the specific monoclonal antibodies against CD68 (DAKO, clone PG-M1, dilution 1:50) Leukocyte common antigen LCA IHC (DAKO, clone 2B11 +PD7/26, dilution 1:200), β1-AR (Elabscience®, ADRB1 polyclonal antibody, dilution 1:200), β2-AR (Elabscience®, ADRB2 polyclonal antibody, dilution 1:200), GRK2 (Elabscience®, GRK2 polyclonal antibody, dilution 1:300), and GRK3 (Elabscience®, GRK3 polyclonal antibody, dilution 1:300).

Techniques: Expressing, Control

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Paroxetine mitigates cardiac remodelling by doxorubicin and increases survival.

doi: 10.1016/j.biopha.2021.112411

Figure Lengend Snippet: Fig. 6. Representative photomicrographs of immunohistochemical analysis showing β1-AR and β2-AR. Note decreased immunoreactivity in DOX+P rats. β-AR, beta- adrenergic receptor; DOX, doxorubicin; P, paroxetine.

Article Snippet: Immunohistochemistry was performed manually on the 5 μm-thick sections, according to the manufacturer’s instructions using the specific monoclonal antibodies against CD68 (DAKO, clone PG-M1, dilution 1:50) Leukocyte common antigen LCA IHC (DAKO, clone 2B11 +PD7/26, dilution 1:200), β1-AR (Elabscience®, ADRB1 polyclonal antibody, dilution 1:200), β2-AR (Elabscience®, ADRB2 polyclonal antibody, dilution 1:200), GRK2 (Elabscience®, GRK2 polyclonal antibody, dilution 1:300), and GRK3 (Elabscience®, GRK3 polyclonal antibody, dilution 1:300).

Techniques: Immunohistochemical staining

Journal: Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Article Title: Paroxetine mitigates cardiac remodelling by doxorubicin and increases survival.

doi: 10.1016/j.biopha.2021.112411

Figure Lengend Snippet: Fig. 7. Relative mRNA expression of cardiac β-1 AR, β-2 AR, GRK2, GRK3, ARRB1, and ARRB2 in DOX- and DOX+P -treated rats. Note a statistically significant increase of GRK2 and GRK3 mRNA levels in DOX-HCM rats, a statistically significant increase of β1-AR and β2-AR mRNA levels, and a statistically significant decrease of GRK2 and GRK3 mRNA levels in DOX+P-treated rats. Data represent the mean of at least 7 rats ± SEM.*p < 0.05; * *p < 0.01 vs. control group, †p < 0.05; ††p < 0.01 vs. DOX-HCM, #p < 0.05; ##p < 0.01 vs. DOX-DCM (One-way ANOVA followed by Tukey’s multiple comparisons or Kruskal–Wallis ANOVA followed by Mann–Whitney U test for nonparametric data). β-AR, beta-adrenergic receptor; DCM, dilated cardiomyopathy; DOX, doxorubicin; HCM, hypertrophic cardiomy opathy; GRK, G protein-coupled receptor kinase; P, paroxetine.

Article Snippet: Immunohistochemistry was performed manually on the 5 μm-thick sections, according to the manufacturer’s instructions using the specific monoclonal antibodies against CD68 (DAKO, clone PG-M1, dilution 1:50) Leukocyte common antigen LCA IHC (DAKO, clone 2B11 +PD7/26, dilution 1:200), β1-AR (Elabscience®, ADRB1 polyclonal antibody, dilution 1:200), β2-AR (Elabscience®, ADRB2 polyclonal antibody, dilution 1:200), GRK2 (Elabscience®, GRK2 polyclonal antibody, dilution 1:300), and GRK3 (Elabscience®, GRK3 polyclonal antibody, dilution 1:300).

Techniques: Expressing, Control, MANN-WHITNEY

Journal: Advanced Science

Article Title: Pharmacological Microglial Inhibition Remodels the Scar Microenvironment to Support Reticulospinal Circuit Reconstruction After Spinal Cord Injury

doi: 10.1002/advs.202503966

Figure Lengend Snippet: Adrb2 agonist inhibit microglial activity in SCI environment. a) Schematic diagram of the experimental design with microglial morphology before and after injury (0.5 mg/kg Adrb2 agonist was administered via intraperitoneal injection). b) Representative pictures showing morphological differences between resting and activated microglia. c) Representative sections showing the microglial activation. d) Quantification of microglial activation ratio (n = 3/3 mice). e) Representative showing macrophage morphology. f) Quantification of macrophage functional differences (cells were plated at a density of 2 × 10⁴ cells/well in 24‐well plates and treated with 100 ng/mL LPS for 24 h prior to analysis. Data represent from n = 3 independent cultures, with 3 technical replicates per condition). g) Schematic diagram of the experimental design for (h—k). h) Representative sections showing the activated states of microglia in lesion center. i) Representative sections showing the resting states of microglia in lesion center. j) Representative sections showing the resting states of microglia in spared neural tissue. k) Quantification of the CD68 + and P2Y12 + microglia at 1, 4, 7 dpi (n = 5/5 mice). l) Representative sections showing the activated and the resting states of microglia at 14 dpi. m) Quantification of the CD68 + and P2Y12 + microglia at 14 dpi (n = 5/5 mice). n) Schematic diagram of the experimental design for bulk RNA‐seq. o) Volcano plots of the injury group and the treatment group. p) GO analysis of the down regulated genes. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. Two‐tailed unpaired t ‐test (d, f, k, m). Data are shown as mean ±s.e.m.

Article Snippet: The experimental animals included C57BL/6 wild‐type (Vital River Laboratory, China), Cx3cr1 GFP (Jackson Laboratory, USA), Cx3cr1 CreERT2 (Jackson Laboratory, USA), Adrb2 flox (Cyagen, China) and Adrb2 −/− (Cyagen, China) strains.

Techniques: Activity Assay, Injection, Activation Assay, Functional Assay, RNA Sequencing, Two Tailed Test

Journal: Advanced Science

Article Title: Pharmacological Microglial Inhibition Remodels the Scar Microenvironment to Support Reticulospinal Circuit Reconstruction After Spinal Cord Injury

doi: 10.1002/advs.202503966

Figure Lengend Snippet: Microglial inhibition therapy remodels the scar microenvironment after SCI. a) Schematic diagram of the experimental design for microglial inhibition therapy. b) Representative sections showing the ECM. c) Quantification of the indicated immunoreactive intensity (n = 4/4 mice). d) Representative sections showing the fibrotic scar. e) Quantification of the Collagen I immunoreactive intensity and the lesion volume (n = 4/4 mice). f) Representative sections showing the spatial relationship between microglia and astrocyte. g) Quantification of the density of IBA1 + microglia in the lesion site (n = 5/5 mice). h) Representative sections showing NF‐200 and NeuN in different groups at 42 dpi. i) Quantification of the neuron number and NF‐200 immunoreactive intensity (n = 3/3 mice). j) Representative sections showing astrocyte scar in different groups at 42 dpi. k) Quantification of the lesion volume in different groups (n = 3/3/3/3 mice). l) Representative sections showing the ECM of the lesion site in different groups at 42 dpi. m) Quantification of the indicated immunoreactive intensity in the lesion site (n = 3/3/3/3 mice). n) Schematic diagram of the experimental design for Adrb2‐cKO. o) Representative sections showing the lesion site in different groups at 42 dpi. p) Quantification of the CSPG immunoreactive intensity and the lesion volume (n = 3/3/3/3 mice). ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001. Two‐tailed unpaired t ‐test (c, e, g, i). One‐way ANOVA, followed by post hoc Bonferroni correction (k, m, p). Data are shown as mean ±s.e.m.

Article Snippet: The experimental animals included C57BL/6 wild‐type (Vital River Laboratory, China), Cx3cr1 GFP (Jackson Laboratory, USA), Cx3cr1 CreERT2 (Jackson Laboratory, USA), Adrb2 flox (Cyagen, China) and Adrb2 −/− (Cyagen, China) strains.

Techniques: Inhibition, Two Tailed Test

Journal: Advanced Science

Article Title: Pharmacological Microglial Inhibition Remodels the Scar Microenvironment to Support Reticulospinal Circuit Reconstruction After Spinal Cord Injury

doi: 10.1002/advs.202503966

Figure Lengend Snippet: Adrb2 agonist mediate the promotion of motor functional recovery via acting on microglia. a) Schematic diagram of the experimental design for behaviour testing. b) Quantification of the BMS scores in different groups (n = 5/5/5/5 mice). c) Quantification of the iliac crest height, stepping distance and toe height (n = 5/5/5/5 mice). d) Trajectory of mice in open field chambers in different groups. e) Quantification of the total distance and maximum speed in open field test (n = 5/5/5/5 mice). f) Representative sections showing c‐Fos + NeuN + cells in L2 sections. g) Quantification of c‐Fos + NeuN + cells in L2 sections (n = 5/5/5/5 mice). * p < 0.05, ** p < 0.01, *** p < 0.001. One‐way ANOVA, followed by post hoc Bonferroni correction (c, e, g). Data are shown as mean ±s.e.m.

Article Snippet: The experimental animals included C57BL/6 wild‐type (Vital River Laboratory, China), Cx3cr1 GFP (Jackson Laboratory, USA), Cx3cr1 CreERT2 (Jackson Laboratory, USA), Adrb2 flox (Cyagen, China) and Adrb2 −/− (Cyagen, China) strains.

Techniques: Functional Assay

Journal: Advanced Science

Article Title: Pharmacological Microglial Inhibition Remodels the Scar Microenvironment to Support Reticulospinal Circuit Reconstruction After Spinal Cord Injury

doi: 10.1002/advs.202503966

Figure Lengend Snippet: Schematic diagram of microglial inhibition therapy. Adrb2 agonist administration post‐SCI suppresses microglial activation, reduces ECM deposition within the scar microenvironment, and attenuates reactive astrogliosis. This permissive microenvironmental remodeling facilitates ReST axonal regeneration across lesion epicenters, reconstructs functional synapses with the caudal spinal circuits, and ultimately restores motor function.

Article Snippet: The experimental animals included C57BL/6 wild‐type (Vital River Laboratory, China), Cx3cr1 GFP (Jackson Laboratory, USA), Cx3cr1 CreERT2 (Jackson Laboratory, USA), Adrb2 flox (Cyagen, China) and Adrb2 −/− (Cyagen, China) strains.

Techniques: Inhibition, Activation Assay, Functional Assay

Journal: Life

Article Title: Sympathetic Nervous Influences Are Negative Prognostic Factors in Stomach Cancer

doi: 10.3390/life14030368

Figure Lengend Snippet: Clinicopathological features.

Article Snippet: Finally, the slices were incubated with the primary antibodies at a temperature of 4 °C for 18 h. The primary antibodies used in our study were anti beta 2 adrenoreceptors (B2A, clone NBP1-90227, dilution 1:100; Novus Biological, Abingdon, UK), tyrosine hydroxylase (TH, clone NB300-109, dilution 1:50; Novus Biological, Abingdon, UK), and anti Ki76 (clone MIB-1, dilution 1:50; Dako, Glostrup, Denmark).

Techniques:

Journal: Life

Article Title: Sympathetic Nervous Influences Are Negative Prognostic Factors in Stomach Cancer

doi: 10.3390/life14030368

Figure Lengend Snippet: Representative pictures showing the immunoreactivity of beta 2 adrenergic receptors in normal peritumor gastric tissue ( A ) and in well-differentiated gastric tumor tissue—G1 ( B ), moderately differentiated tissue—G2 ( C ) and poorly differentiated tissue—G3 ( D ), 40×. ( A′ – D′ ) represent the mixed spectral compound. ( A″ – D″ ) represent the color channel only for beta 2 adrenoreceptors.

Article Snippet: Finally, the slices were incubated with the primary antibodies at a temperature of 4 °C for 18 h. The primary antibodies used in our study were anti beta 2 adrenoreceptors (B2A, clone NBP1-90227, dilution 1:100; Novus Biological, Abingdon, UK), tyrosine hydroxylase (TH, clone NB300-109, dilution 1:50; Novus Biological, Abingdon, UK), and anti Ki76 (clone MIB-1, dilution 1:50; Dako, Glostrup, Denmark).

Techniques:

Journal: Life

Article Title: Sympathetic Nervous Influences Are Negative Prognostic Factors in Stomach Cancer

doi: 10.3390/life14030368

Figure Lengend Snippet: Immunoreactivity in gastric cancer for beta 2 adrenoreceptors—B2A ( A ), Ki 67 ( B ), and both intra-tumoral ( C ) and extra-tumoral ( D ) tyrosine hydroxylase. G1—well-differentiated gastric tumor tissue, G2—moderately differentiated tissue, and G3—poorly differentiated tissue. * represents statistically significant, ** represents highly significant and **** represents extremely significant.

Article Snippet: Finally, the slices were incubated with the primary antibodies at a temperature of 4 °C for 18 h. The primary antibodies used in our study were anti beta 2 adrenoreceptors (B2A, clone NBP1-90227, dilution 1:100; Novus Biological, Abingdon, UK), tyrosine hydroxylase (TH, clone NB300-109, dilution 1:50; Novus Biological, Abingdon, UK), and anti Ki76 (clone MIB-1, dilution 1:50; Dako, Glostrup, Denmark).

Techniques:

Journal: Life

Article Title: Sympathetic Nervous Influences Are Negative Prognostic Factors in Stomach Cancer

doi: 10.3390/life14030368

Figure Lengend Snippet: Correlations between intra-tumoral and extra-tumoral tyrosine hydroxylase immunoreactivity ( A ), between beta 2 adrenoreceptors and Ki 67 ( B ), between intra-tumoral tyrosine hydroxylase and Ki 67 ( C ), and between extra-tumoral tyrosine hydroxylase and Ki 67 ( D ).

Article Snippet: Finally, the slices were incubated with the primary antibodies at a temperature of 4 °C for 18 h. The primary antibodies used in our study were anti beta 2 adrenoreceptors (B2A, clone NBP1-90227, dilution 1:100; Novus Biological, Abingdon, UK), tyrosine hydroxylase (TH, clone NB300-109, dilution 1:50; Novus Biological, Abingdon, UK), and anti Ki76 (clone MIB-1, dilution 1:50; Dako, Glostrup, Denmark).

Techniques:

Journal: Life

Article Title: Sympathetic Nervous Influences Are Negative Prognostic Factors in Stomach Cancer

doi: 10.3390/life14030368

Figure Lengend Snippet: Kaplan–Meier curves depending on beta 2 adrenoreceptors immunoreactivity ( A ), intra-tumoral tyrosine hydroxylase (Th) immunoreactivity ( B ), and extra-tumoral tyrosine hydroxylase (Th) immunoreactivity ( C ).

Article Snippet: Finally, the slices were incubated with the primary antibodies at a temperature of 4 °C for 18 h. The primary antibodies used in our study were anti beta 2 adrenoreceptors (B2A, clone NBP1-90227, dilution 1:100; Novus Biological, Abingdon, UK), tyrosine hydroxylase (TH, clone NB300-109, dilution 1:50; Novus Biological, Abingdon, UK), and anti Ki76 (clone MIB-1, dilution 1:50; Dako, Glostrup, Denmark).

Techniques: