Journal: bioRxiv

Article Title: Germ-free piglets display variable neuroinflammatory-like perturbations in prefrontal cortical microglia

doi: 10.64898/2026.03.22.713463

Figure Lengend Snippet: (A) Volcano plot of significant upregulated and downregulated genes in GF tissue, using p < 0.05 and a threshold of 1.2-fold change in expression as a cutoff for analysis. CD38 (p < 0.001, FC = 1.55), RSAD2 (p < 0.001, FC = 0.08), GABRR1 (p < 0.001, FC = 0.08), FN1 (p < 0.001, FC = 0.54), IFI6 (p < 0.001, FC = 0.37), IRAK1BP1 (p < 0.001, FC = 1.74), RGS1 (p < 0.001, FC = 3.22) are labeled in bold. (B) Graphical reconstruction of immune activation Ingenuity Pathway Analysis result, showing differentially expressed genes in our tissue related to this pathway. Panel made with BioRender. (C) Representative immunohistochemical images of the PFCII-III in control and GF animals. CD38 + (red), DAPI + (blue), and Iba1 + (white) staining (left, scale bar 200µm), a middle inset of the leftmost image (middle, scale bar 50µm), and a single channel (CD38) of this inset (right, scale bar 50µm) for each group n=3/animals per group. Data supported by Extended Data Table 6-1.

Article Snippet: Sections were blocked with 5% donkey serum, 1% BSA in PBST for 1 hour, and then stained with Iba1 (Wako, catalog #NC9288364), Ki67 (Abcam, catalog #ab16667), and CD38 (Proteintech, catalog #60006-1-Ig) overnight at 4°C.

Techniques: Expressing, Labeling, Activation Assay, Immunohistochemical staining, Control, Staining