Journal: Diseases

Article Title: Assessing Anti-Adipogenic Effects of Mango Leaf Tea and Mangiferin within Cultured Adipocytes

doi: 10.3390/diseases11020070

Figure Lengend Snippet: Incubating cultured adipocytes with MLT augments the secretion of adiponectin and enhances glucose uptake. ( A , B ) Adipocytes were treated with vehicle, MGF, or MLT. ELISA was used to measure secretory adiponectin ( A ), and a fluorometric kit was used for measuring glucose uptake of fluorescently labeled 2-NBDG ( B ). ( A ) Four independent experiments were conducted using one biological replicate for each experiment. ( B ) Two independent experiments were performed, each with triplicate biological replicates used. ( A , B ) Bars are group means.

Article Snippet: We measured adiponectin in the centrifuged cell culture medium using a mouse adiponectin ELISA kit (AccuSignal TM ELISA Kit, KOA0366; Rockland, Limerick, PA, USA), following the manufacturer’s instructions.

Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Labeling