Journal: Cancer Cell

Article Title: Retinoic acid receptor activation reprograms senescence response and enhances anti-tumor activity of natural killer cells

doi: 10.1016/j.ccell.2024.02.004

Figure Lengend Snippet:

Article Snippet: Adapalene , MedChem Express , Cat# HY-B0091.

Techniques: Control, Purification, Recombinant, Staining, Reverse Transcription, Cell Isolation, Isolation, Expressing, shRNA, Plasmid Preparation, CRISPR, Software, Transfection, In Vitro, Imaging

Journal: Frontiers in Chemistry

Article Title: Environmentally sensitive fluorescence of the topical retinoid adapalene

doi: 10.3389/fchem.2024.1438751

Figure Lengend Snippet: Adapalene chemical structure.

Article Snippet: Adapalene was purchased from BIOSYNTH Carbosynth and was used without further purification.

Techniques:

Journal: Frontiers in Chemistry

Article Title: Environmentally sensitive fluorescence of the topical retinoid adapalene

doi: 10.3389/fchem.2024.1438751

Figure Lengend Snippet: Normalized UVA absorption band in the different solvents. ( acetonitrile, ethyl acetate, 1,4-dioxane, ethanol, hexane, methanol, dichloromethane, DMF, THF). Inset: Normalized UV-vis absorption spectra of adapalene in acetonitrile, ethanol and hexane.

Article Snippet: Adapalene was purchased from BIOSYNTH Carbosynth and was used without further purification.

Techniques:

Journal: Frontiers in Chemistry

Article Title: Environmentally sensitive fluorescence of the topical retinoid adapalene

doi: 10.3389/fchem.2024.1438751

Figure Lengend Snippet: Absorption (λ A , nm) and fluorescence (λ F , nm) maxima, Stokes shift (Δν, cm −1 ) of adapalene in solvents of different polarity (dielectric constant, є, refractive index, n, and E T (30) parameter).

Article Snippet: Adapalene was purchased from BIOSYNTH Carbosynth and was used without further purification.

Techniques: Fluorescence, Refractive Index

Journal: Frontiers in Chemistry

Article Title: Environmentally sensitive fluorescence of the topical retinoid adapalene

doi: 10.3389/fchem.2024.1438751

Figure Lengend Snippet: Absorption of adapalene alone (10 μM, gray), DNA (50 μM in bp, dotted green line) or adapalene in the presence of DNA (full green line) in PBS at pH 7.4. Inset: adapalene alone (10 μM, gray), HSA alone (2 μM, dotted blue line), of adapalene in the presence of HSA (full blue line).

Article Snippet: Adapalene was purchased from BIOSYNTH Carbosynth and was used without further purification.

Techniques:

Journal: Frontiers in Chemistry

Article Title: Environmentally sensitive fluorescence of the topical retinoid adapalene

doi: 10.3389/fchem.2024.1438751

Figure Lengend Snippet: Normalized fluorescence spectra of adapalene in different solvents.

Article Snippet: Adapalene was purchased from BIOSYNTH Carbosynth and was used without further purification.

Techniques: Fluorescence

Journal: Frontiers in Chemistry

Article Title: Environmentally sensitive fluorescence of the topical retinoid adapalene

doi: 10.3389/fchem.2024.1438751

Figure Lengend Snippet: (A) Position of the emission maxima in wavenumber of adapalene versus the polarity E T (30) in different solvents or in dioxane:ethanol mixtures (inset). (B) Lippert-Mataga plot for adapalene in different solvents or in dioxane:ethanol mixtures (inset).

Article Snippet: Adapalene was purchased from BIOSYNTH Carbosynth and was used without further purification.

Techniques:

Journal: Frontiers in Chemistry

Article Title: Environmentally sensitive fluorescence of the topical retinoid adapalene

doi: 10.3389/fchem.2024.1438751

Figure Lengend Snippet: Fluorescence emission (λ exc = 315 nm) of a solution containing adapalene (10 μM) and HSA (12 μM) in PBS at pH 7.4, adapalene alone in PBS (gray dotted line). Inset emission changes as a function of the protein concentration.

Article Snippet: Adapalene was purchased from BIOSYNTH Carbosynth and was used without further purification.

Techniques: Fluorescence, Protein Concentration

Journal: Frontiers in Chemistry

Article Title: Environmentally sensitive fluorescence of the topical retinoid adapalene

doi: 10.3389/fchem.2024.1438751

Figure Lengend Snippet: Fluorescence emission (λ exc = 315 nm) of adapalene (10 μM) alone in PBS at pH 7.4 (black) and in the presence of duplex salmon sperm DNA (from 25 to 400 μM in bp). Inset: Stern-Volmer plot for the quenching of adapalene fluorescence in PBS (blue), with 10 μM bp (orange) or 100 μM bp (grey) of DNA.

Article Snippet: Adapalene was purchased from BIOSYNTH Carbosynth and was used without further purification.

Techniques: Fluorescence

Journal: Frontiers in Chemistry

Article Title: Environmentally sensitive fluorescence of the topical retinoid adapalene

doi: 10.3389/fchem.2024.1438751

Figure Lengend Snippet: Emission spectra of adapalene (10 μM) varying CTAB concentration (from 0.05 to 4 mM) in PBS and adapalene alone in PBS (gray dotted line), λ exc = 315 nm. Inset: Representation of the changes of 390 nm emission vs. the logarithm of CTAB concentration.

Article Snippet: Adapalene was purchased from BIOSYNTH Carbosynth and was used without further purification.

Techniques: Concentration Assay

Journal: Journal of Lipid Research

Article Title: Library screening identifies commercial drugs as potential structure correctors of abnormal apolipoprotein A-I

doi: 10.1016/j.jlr.2024.100543

Figure Lengend Snippet: Binding of hydrophobic fluorescent probe ANS on WT apoA-I and apoA-I[L178P] in the presence of compounds from the Pfizer licensed compound library (A, C) and the FDA-approved drug library (B, D, E, and F). A, B: ratio of fluorescence signal of ANS binding to WT apoA-I against the fluorescence signal of ANS binding to apoA-I[L178P] (both proteins were at a concentration of 3.6 μM) in the absence or presence of selected compounds (0.1 mM) (compounds code names are described in supplemental Figs. S1H and S2L). ## P < 0.01 and ∗∗ P < 0.005 versus proteins measured in the absence of compounds. C–F: ANS fluorescence spectra of WT or mutant apoA-I in the presence or absence of 0.1 mM atorvastatin (C8), bexarotene (C9-p1), adapalene (C4-p2), and lovastatin (G9-p11). ANS, 8-anilinonaphthalene-1-sulfonic acid; AU, arbitrary units; Comp, compound; FDA, Food and Drug Administration.

Article Snippet: Food and Drug Administration (FDA)-approved drugs screening library (875 FDA-approved drug compounds supplied as predissolved DMSO solutions at a concentration of 10 mM), atorvastatin (calcium salt), bexarotene, adapalene, and lovastatin were from Cayman Chemical.

Techniques: Binding Assay, Drug discovery, Fluorescence, Concentration Assay, Mutagenesis

Journal: Journal of Lipid Research

Article Title: Library screening identifies commercial drugs as potential structure correctors of abnormal apolipoprotein A-I

doi: 10.1016/j.jlr.2024.100543

Figure Lengend Snippet: Effect of atorvastatin, bexarotene, and adapalene on J774 macrophages viability. Survival of J774 macrophages incubated with increasing concentrations of atorvastatin (A), bexarotene (B), and adapalene (C) for 4 h, as determined by a MTT assay. Cell viability is expressed as percent relative to the viability of untreated (control) cells set to 100%. Data are the means ± SD of three experiments performed in duplicate. ∗ P < 0.05, ∗∗ P < 0.005, and ∗∗∗ P < 0.0001 versus untreated cells. MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide.

Article Snippet: Food and Drug Administration (FDA)-approved drugs screening library (875 FDA-approved drug compounds supplied as predissolved DMSO solutions at a concentration of 10 mM), atorvastatin (calcium salt), bexarotene, adapalene, and lovastatin were from Cayman Chemical.

Techniques: Incubation, MTT Assay, Control