Journal: Annals of Translational Medicine

Article Title: MicroRNA-25-3p therapy for intervertebral disc degeneration by targeting the IL-1β/ZIP8/MTF1 signaling pathway with a novel thermo-responsive vector

doi: 10.21037/atm-20-6595

Figure Lengend Snippet: The influence of IL-1β on ECM-degrading enzyme (MMP3, MMP13 and ADAMTS5) and ECM protein (collagen type II and aggrecan) expression. (A) Relative expression levels of MMP3 mRNA, MMP13 mRNA and ADAMTS5 mRNA in IL-1β-stimulated nucleus pulposus (NP) cells (10 ng/mL) and control cells via qRT-PCR. (B) Relative protein expression levels of MMP3, MMP13 and ADAMTS5 were examined in IL-1β-stimulated NP cells (10 ng/mL) and control cells via western blotting. (C) Relative protein expression levels of collagen type II and aggrecan were detected in IL-1β-stimulated NP cells (10 ng/mL) and control cells via immunocytochemistry (ICC). The bar is 50 µm. Data are the mean ± standard deviation (SD) of triplicate results. *, P<0.05.

Article Snippet: Membranes were incubated overnight at 4 °C with anti-IL-1β antibody (12703, 1:1,000, CST, USA), anti-MTF1 antibody (ab184119, 1:1,000, Abcam, Cambridge, UK), anti-ZIP8 antibody (NBP1-76505, 1:2,000, Novus, Biologicals, Colorado, USA), anti-MMP3 antibody (NB100-91878, 1:1,000, Novus Biologicals, Colorado, USA), anti-MMP13 antibody (NBP2-45887, 1:1,000, Novus, Biologicals, Colorado, USA), anti-ADAMTS5 antibody (NBP2-15286, 1:1,000, Novus, Biologicals, Colorado,USA), anti-β-actin antibody (abs118937, 1:3,000, Absin, Shanghai, China), and anti-PCNA antibody (abs13075, 1:2,000, Absin, Shanghai, China).

Techniques: Expressing, Control, Quantitative RT-PCR, Western Blot, Immunocytochemistry, Standard Deviation

Journal: Annals of Translational Medicine

Article Title: MicroRNA-25-3p therapy for intervertebral disc degeneration by targeting the IL-1β/ZIP8/MTF1 signaling pathway with a novel thermo-responsive vector

doi: 10.21037/atm-20-6595

Figure Lengend Snippet: The effects of miRNA-25-3p modulation on IL-1β-stimulated ECM-degrading enzyme (MMP3, MMP13 and ADAMTS5) and ECM protein (collagen type II and aggrecan) expression. (A) Relative mRNA expression levels of MMP3, MMP13 and ADAMTS5 in the miRNA-25-3p mimic group and the control group determined via qRT-PCR. (B) Relative protein expression levels of MMP3, MMP13 and ADAMTS5 were examined in the miRNA-25-3p mimic group and the control group via western blotting. (C) Relative protein expression levels of collagen type II and aggrecan were detected in the miRNA-25-3p mimic group and the control group via immunocytochemistry (ICC). The bar is 50 µm. Data are the mean ± standard deviation (SD) of triplicate results. *, P<0.05.

Article Snippet: Membranes were incubated overnight at 4 °C with anti-IL-1β antibody (12703, 1:1,000, CST, USA), anti-MTF1 antibody (ab184119, 1:1,000, Abcam, Cambridge, UK), anti-ZIP8 antibody (NBP1-76505, 1:2,000, Novus, Biologicals, Colorado, USA), anti-MMP3 antibody (NB100-91878, 1:1,000, Novus Biologicals, Colorado, USA), anti-MMP13 antibody (NBP2-45887, 1:1,000, Novus, Biologicals, Colorado, USA), anti-ADAMTS5 antibody (NBP2-15286, 1:1,000, Novus, Biologicals, Colorado,USA), anti-β-actin antibody (abs118937, 1:3,000, Absin, Shanghai, China), and anti-PCNA antibody (abs13075, 1:2,000, Absin, Shanghai, China).

Techniques: Expressing, Control, Quantitative RT-PCR, Western Blot, Immunocytochemistry, Standard Deviation

Journal: Molecular Medicine Reports

Article Title: Detection of miR-29a in plasma of patients with lumbar spinal stenosis and the clinical significance

doi: 10.3892/mmr.2018.8956

Figure Lengend Snippet: Comparison of plasma levels of (A) MMP9 and (B) ADAMTS5 between different patient groups. Triangles and black dots represent the ROC curve of LDH and LSS, respectively. The gray lines are lines of identity. **P<0.01, compared with the control group. ## P<0.01, compared with patients with LDH. ADAMTS5; a disintegrin and metalloproteinase with thrombospondin motifs 5; control, healthy patients; LDH, lumbar intervertebral disc herniation; LSS, lumbar spinal stenosis; MMP9, matrix metalloproteinase 9.

Article Snippet: Human MMP-9 Quantikine ELISA kit DMP900 (R&D Systems, Inc., Minneapolis, MN, USA) and Human ADAMTS5 DuoSet ELISA kit (R&D Systems, Inc.) were used according to the manufacture's protocols.

Techniques: Comparison, Clinical Proteomics, Control

Journal: Molecular Medicine Reports

Article Title: Detection of miR-29a in plasma of patients with lumbar spinal stenosis and the clinical significance

doi: 10.3892/mmr.2018.8956

Figure Lengend Snippet: Comparison of MMP9 and ADAMTS5 expression levels in intervertebral disc tissue of various patient groups. (A) Expression levels of MMP9 and ADAMTS5 in intervertebral disc tissue of various patient groups; (B) Expression levels of ADAMTS5 in intervertebral disc tissue of various patient groups. **P<0.01, compared with the control group. ## P<0.01, compared with patients with LDH. ADAMTS5; a disintegrin and metalloproteinase with thrombospondin motifs 5; control, healthy patients; LDH, lumbar intervertebral disc herniation; LSS, lumbar spinal stenosis; MMP9, matrix metalloproteinase 9.

Article Snippet: Human MMP-9 Quantikine ELISA kit DMP900 (R&D Systems, Inc., Minneapolis, MN, USA) and Human ADAMTS5 DuoSet ELISA kit (R&D Systems, Inc.) were used according to the manufacture's protocols.

Techniques: Comparison, Expressing, Control

Journal: Molecular Medicine Reports

Article Title: Detection of miR-29a in plasma of patients with lumbar spinal stenosis and the clinical significance

doi: 10.3892/mmr.2018.8956

Figure Lengend Snippet: Correlations between the expression levels of miR-29, MMP9 and ADAMTS5, and correlation between their expression levels in plasma and intervertebral disc tissue of LSS patients. (A) Correlation between the expression levels of miR-29 and MMP9. (B) Correlation between the expression levels of miR-29 and ADAMTS5. (C) Correlation between the expression levels of miR-29 in plasma and intervertebral disc tissue. Correlation between the expression levels of (D) MMP9 and (E) ADAMTS5 in plasma and intervertebral disc tissue. y, the binary equation representing the distributions of values; ADAMTS5; a disintegrin and metalloproteinase with thrombospondin motifs 5; MMP9, matrix metalloproteinase 9.

Article Snippet: Human MMP-9 Quantikine ELISA kit DMP900 (R&D Systems, Inc., Minneapolis, MN, USA) and Human ADAMTS5 DuoSet ELISA kit (R&D Systems, Inc.) were used according to the manufacture's protocols.

Techniques: Expressing, Clinical Proteomics