Journal: Cell metabolism

Article Title: Targeting Peripheral CB 1 Receptors Reduces Ethanol Intake via a Gut-Brain Axis

doi: 10.1016/j.cmet.2019.04.012

Figure Lengend Snippet: (A) Time course and rate of increase in oleate-driven respiratory activity in MGN3–1 cells. Cells were treated with 10 or 100 nM JD5037, 2.5 M of FCCP (positive control) or vehicle in the presence of the fluorescent extracellular O2 consumption reagent. Oxidative respiration was recorded every 90 sec and the slope of the initial linear increase was calculated. Points and bars are means ± SEM from n = 11–15 experiments, as indicated. *P < 0.05 compared to vehicle. (B) Verification of Cnr1 knockdown by rtPCR in cells used in panel C. Cellular uptake of the constructs was verified by fluorescent microscopy (20x magnification) and the degree of knockdown was determined by rt-PCR, *P < 0.05, n = 4. (C) Oleate-driven oxidative activity in MGN3–1 cells with shRNA-mediated knockdown of Cnr1 expression and their mock-transfected controls, n = 3–8 experiments, as indicated, *P < 0.05.

Article Snippet: Scramble shRNA with GFP Adenovirus (Ad-scramble-shRNA) , Vector Biolabs , 1122.

Techniques: Activity Assay, Positive Control, Knockdown, Reverse Transcription Polymerase Chain Reaction, Construct, Microscopy, shRNA, Expressing, Transfection

Journal: Lipids in Health and Disease

Article Title: Heme oxygenase-1 prevents non-alcoholic steatohepatitis through suppressing hepatocyte apoptosis in mice

doi: 10.1186/1476-511X-9-124

Figure Lengend Snippet: Effect of HO-1 on hepatocyte apoptosis in mice fed with MCD diet at four weeks . (A) TUNEL staining for hepatocyte apoptosis in liver sections from mice administrated: control diet; MCD diet; MCD diet treated with hemin, ZnPP-IX, Ad-GFP, Ad-HO-1 and combination of Ad-HO-1 and hemin, respectively. (original magnification, × 400). (B) Quantitation of mean TUNEL-positive cells/field. are expressed as mean ± SD. * P < 0.01 compared with control; # P < 0.05, ## P < 0.01, compared with MCD feeding mice; $ P < 0.01, compared with MCD+Ad-GFP treated mice. Slides are representative of 6 animals per group.

Article Snippet: A recombinant adenovirus containing the entire coding sequence of mouse HO-1 (Ad-HO-1) and control adenovirus encoding green fluorescent protein (Ad-GFP) were purchased from Tianjin Saier Biochemistry company limited (Tianjin, China).

Techniques: TUNEL Assay, Staining, Quantitation Assay

Journal: Lipids in Health and Disease

Article Title: Heme oxygenase-1 prevents non-alcoholic steatohepatitis through suppressing hepatocyte apoptosis in mice

doi: 10.1186/1476-511X-9-124

Figure Lengend Snippet: Effects of the MCD diet and treatment with hemin and/or Ad-HO-1 or ZnPP-IX on hepatic lipoperoxide content measured as thiobarbituric acid-reactive substrances (TBARS) . Data are expressed as mean ± SD (n = 6 per group). * P < 0.01, ** P < 0.001, compared with control; # P < 0.05, ## P < 0.01, compared with MCD feeding mice; $ P < 0.05, $$ P < 0.01, compared with MCD+ Ad-GFP treated mice.

Article Snippet: A recombinant adenovirus containing the entire coding sequence of mouse HO-1 (Ad-HO-1) and control adenovirus encoding green fluorescent protein (Ad-GFP) were purchased from Tianjin Saier Biochemistry company limited (Tianjin, China).

Techniques:

Journal: Lipids in Health and Disease

Article Title: Heme oxygenase-1 prevents non-alcoholic steatohepatitis through suppressing hepatocyte apoptosis in mice

doi: 10.1186/1476-511X-9-124

Figure Lengend Snippet: Effects of hemin and/or Ad-HO-1 on hepatic HO-1 protein expression in the liver of mice . (A) Immunostaining for HO-1 protein. (B) Effect of hemin and/or Ad-HO-1 on quantitative protein expression of HO-1. The expression of HO-1 was estimated by average area density (areas of positive cells/total areas) (original magnification, × 200). Data are expressed as the mean ± SD (n = 6 per group). * P < 0.001, compared with control; # P < 0.01, compared with MCD feeding mice; $ P < 0.01, compared with MCD+ Ad-GFP treated mice.

Article Snippet: A recombinant adenovirus containing the entire coding sequence of mouse HO-1 (Ad-HO-1) and control adenovirus encoding green fluorescent protein (Ad-GFP) were purchased from Tianjin Saier Biochemistry company limited (Tianjin, China).

Techniques: Expressing, Immunostaining

Journal: Lipids in Health and Disease

Article Title: Heme oxygenase-1 prevents non-alcoholic steatohepatitis through suppressing hepatocyte apoptosis in mice

doi: 10.1186/1476-511X-9-124

Figure Lengend Snippet: Effects of HO-1 on hepatic expression of cytochrome p450 2E1 (CYP2E1) (A) and cytochrome c (Cyt-c) (B) . mRNA expression of CYP2E1 (A1) and Cyt-c mRNA(B1) was examined by real-time quantitative PCR ; and protein expression of CYP2E1 (A2) and Cyt-c (B2) were measured by Western blot. Data are expressed as the mean ± SD (n = 6 per group). * P < 0.001, compared with control mice; # P < 0.05, ## P < 0.01, ### P < 0.001, compared with MCD mice; $ P < 0.05, $$ P < 0.01, compared with MCD+ Ad-GFP treated mice.

Article Snippet: A recombinant adenovirus containing the entire coding sequence of mouse HO-1 (Ad-HO-1) and control adenovirus encoding green fluorescent protein (Ad-GFP) were purchased from Tianjin Saier Biochemistry company limited (Tianjin, China).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: Lipids in Health and Disease

Article Title: Heme oxygenase-1 prevents non-alcoholic steatohepatitis through suppressing hepatocyte apoptosis in mice

doi: 10.1186/1476-511X-9-124

Figure Lengend Snippet: Effects of HO-1 on expression of FAS and FASL in the liver of mice . mRNA expression of Fas (A1) and Fasligand (FasL) (B1) was examined by real-time PCR; protein expression of Fas (A2) and FasL (B2) were measured by Western blot. Data are expressed as the mean ± SD (n = 6 per group). * P < 0.001, compared with control mice; # P < 0.05, ## P < 0.01, compared with MCD mice; $ P < 0.05, $$ P < 0.01, $$$ P < 0.01, compared with MCD+ Ad-GFP treated mice.

Article Snippet: A recombinant adenovirus containing the entire coding sequence of mouse HO-1 (Ad-HO-1) and control adenovirus encoding green fluorescent protein (Ad-GFP) were purchased from Tianjin Saier Biochemistry company limited (Tianjin, China).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: Lipids in Health and Disease

Article Title: Heme oxygenase-1 prevents non-alcoholic steatohepatitis through suppressing hepatocyte apoptosis in mice

doi: 10.1186/1476-511X-9-124

Figure Lengend Snippet: Effects of HO-1 on expression of caspase 3 and casepase 9 in the liver of mice . mRNA expression of casepase 3 (A1) and casepase 9 (B1) was examined by real-time PCR ; protein expression of casepase 3 (A2) and casepase 9 (B2) were measured by Western blot. Data are expressed as the mean ± SD (n = 6 per group). * P < 0.01, ** P < 0.001 compared with control mice; # P < 0.05, ## P < 0.01, ### P < 0.01, compared with MCD mice; $ P < 0.05, $$ P < 0.01, $$$ P < 0.01, compared with MCD+ Ad-GFP treated mice.

Article Snippet: A recombinant adenovirus containing the entire coding sequence of mouse HO-1 (Ad-HO-1) and control adenovirus encoding green fluorescent protein (Ad-GFP) were purchased from Tianjin Saier Biochemistry company limited (Tianjin, China).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: Lipids in Health and Disease

Article Title: Heme oxygenase-1 prevents non-alcoholic steatohepatitis through suppressing hepatocyte apoptosis in mice

doi: 10.1186/1476-511X-9-124

Figure Lengend Snippet: Effects of HO-1 on expression of Bax and Bcl-2 in the liver of mice . mRNA expression of Bax (A1) and Bcl-2 (B1) was examined by real-time PCR; protein expression of Bax (A2) and Bcl-2 (B2) were measured by Western blot. Data are expressed as the mean ± SD (n = 6 per group). * P < 0.01, ** P < 0.001 compared with control mice; # P < 0.01, ## P < 0.01, compared with MCD mice; $ P < 0.05, $$ P < 0.01, compared with MCD+ Ad-GFP treated mice.

Article Snippet: A recombinant adenovirus containing the entire coding sequence of mouse HO-1 (Ad-HO-1) and control adenovirus encoding green fluorescent protein (Ad-GFP) were purchased from Tianjin Saier Biochemistry company limited (Tianjin, China).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: Cell reports

Article Title: Pancreatic islet protection at the expense of secretory function involves serine-linked mitochondrial one-carbon metabolism

doi: 10.1016/j.celrep.2023.112615

Figure Lengend Snippet:

Article Snippet: Adenovirus: Ad-GFP control (Ad- GFP ) , ViraQuest Inc , VQAd CMV eGFP.

Techniques: Virus, Control, Plasmid Preparation, Recombinant, Reverse Transcription, Fractionation, Enzyme-linked Immunosorbent Assay, Software, Staining