ad-cmv-luc Search Results


ad cmv luciferase  (Vector Biolabs)
96
Vector Biolabs ad cmv luciferase
Ad Cmv Luciferase, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ad-cmv-luc/Ad-CMV-Luc/pmc04596849-29-0-3
Average 96 stars, based on 1 article reviews
ad cmv luciferase - by Bioz Stars, 2026-10
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adcmvluc  (Promega)
90
Promega adcmvluc
In vitro analyses. ( A ) Analysis of COX-2 RNA levels in prostate cancer cell lines. Total cellular RNA of human prostate cancer cells (LnCap, Du-145, PC3) as well as COX-2 control cells (A549 as positive and BT474 as negative) was reverse-transcribed with oligo-dT primer and amplified by PCR with COX-2—( upper panel) and GAPDH ( lower panel)-specific primers. The signal for correctly spliced cyclooxygenase-2 mRNA was detected as a 723 bp band. ( B ) Activity and selectivity of COX-2 promoter in prostate cancer cells. Human prostate cancer cells (LnCap, Du-145, PC3), COX-2—positive A549 cells, and COX-2-negative BT474 cells were infected with AdCMVLuc, AdCox2MLuc, and AdCox2LLuc. The cells were lysed and assessed for <t>luciferase</t> activity two days after infection. Data are shown as percentages of relative light units (RLUs) per milligram protein in relation to that of CMV-promoter-driven luciferase expression. The Cox2M promoter showed higher activity in Du-145 cells compared to the Cox2L promoter, and both had about the same activity in PC3. Minimal activity was seen in the COX-2-negative prostate cancer cell line (LnCap) and the COX-2-negative control cell line (BT474). Errors bars represent the standard deviation calculated from three replicates. * p < 0.05 ( C ) Transduction efficiency in prostate cancer cells with infectivity-enhanced Ad vectors. Human prostate cancer cells (LnCap, Du-145, PC3), and two control cell lines (A549 and BT474) were infected with CMV-promoter-driven Luc-expressing vectors possessing unmodified, RGD-modified, or Ad5/Ad-3chimeric fibers (Ad5Luc1, Ad5RGDLuc1, and Ad5/3Luc1, respectively). After two days of incubation, the cells were lysed, and the Luc activity was measured. Du-145 and PC3 prostate cancer cell lines demonstrated significantly higher levels of transgene expression with the Ad5/Ad3—modified vector than that with Ad5Luc1. The enhancement was not significant for LnCap. In all three prostate cancer cell lines, the level of transgene expression with an RGD-modified vector tended to be slightly lower than that of Ad5Luc. Errors bars represent the standard deviation calculated from three replicates. * p < 0.05.
Adcmvluc, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ad-cmv-luc/adcmvluc/pmc10144787-58-9-15
Average 90 stars, based on 1 article reviews
adcmvluc - by Bioz Stars, 2026-10
90/100 stars
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adcmvluc  (Marburg GmbH)
90
Marburg GmbH adcmvluc
In vitro analyses. ( A ) Analysis of COX-2 RNA levels in prostate cancer cell lines. Total cellular RNA of human prostate cancer cells (LnCap, Du-145, PC3) as well as COX-2 control cells (A549 as positive and BT474 as negative) was reverse-transcribed with oligo-dT primer and amplified by PCR with COX-2—( upper panel) and GAPDH ( lower panel)-specific primers. The signal for correctly spliced cyclooxygenase-2 mRNA was detected as a 723 bp band. ( B ) Activity and selectivity of COX-2 promoter in prostate cancer cells. Human prostate cancer cells (LnCap, Du-145, PC3), COX-2—positive A549 cells, and COX-2-negative BT474 cells were infected with AdCMVLuc, AdCox2MLuc, and AdCox2LLuc. The cells were lysed and assessed for <t>luciferase</t> activity two days after infection. Data are shown as percentages of relative light units (RLUs) per milligram protein in relation to that of CMV-promoter-driven luciferase expression. The Cox2M promoter showed higher activity in Du-145 cells compared to the Cox2L promoter, and both had about the same activity in PC3. Minimal activity was seen in the COX-2-negative prostate cancer cell line (LnCap) and the COX-2-negative control cell line (BT474). Errors bars represent the standard deviation calculated from three replicates. * p < 0.05 ( C ) Transduction efficiency in prostate cancer cells with infectivity-enhanced Ad vectors. Human prostate cancer cells (LnCap, Du-145, PC3), and two control cell lines (A549 and BT474) were infected with CMV-promoter-driven Luc-expressing vectors possessing unmodified, RGD-modified, or Ad5/Ad-3chimeric fibers (Ad5Luc1, Ad5RGDLuc1, and Ad5/3Luc1, respectively). After two days of incubation, the cells were lysed, and the Luc activity was measured. Du-145 and PC3 prostate cancer cell lines demonstrated significantly higher levels of transgene expression with the Ad5/Ad3—modified vector than that with Ad5Luc1. The enhancement was not significant for LnCap. In all three prostate cancer cell lines, the level of transgene expression with an RGD-modified vector tended to be slightly lower than that of Ad5Luc. Errors bars represent the standard deviation calculated from three replicates. * p < 0.05.
Adcmvluc, supplied by Marburg GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ad-cmv-luc/adcmvluc/pm15170735-70-0-8
Average 90 stars, based on 1 article reviews
adcmvluc - by Bioz Stars, 2026-10
90/100 stars
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ad-cmv-luc  (Vector Biolabs)
N/A
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In vitro analyses. ( A ) Analysis of COX-2 RNA levels in prostate cancer cell lines. Total cellular RNA of human prostate cancer cells (LnCap, Du-145, PC3) as well as COX-2 control cells (A549 as positive and BT474 as negative) was reverse-transcribed with oligo-dT primer and amplified by PCR with COX-2—( upper panel) and GAPDH ( lower panel)-specific primers. The signal for correctly spliced cyclooxygenase-2 mRNA was detected as a 723 bp band. ( B ) Activity and selectivity of COX-2 promoter in prostate cancer cells. Human prostate cancer cells (LnCap, Du-145, PC3), COX-2—positive A549 cells, and COX-2-negative BT474 cells were infected with AdCMVLuc, AdCox2MLuc, and AdCox2LLuc. The cells were lysed and assessed for luciferase activity two days after infection. Data are shown as percentages of relative light units (RLUs) per milligram protein in relation to that of CMV-promoter-driven luciferase expression. The Cox2M promoter showed higher activity in Du-145 cells compared to the Cox2L promoter, and both had about the same activity in PC3. Minimal activity was seen in the COX-2-negative prostate cancer cell line (LnCap) and the COX-2-negative control cell line (BT474). Errors bars represent the standard deviation calculated from three replicates. * p < 0.05 ( C ) Transduction efficiency in prostate cancer cells with infectivity-enhanced Ad vectors. Human prostate cancer cells (LnCap, Du-145, PC3), and two control cell lines (A549 and BT474) were infected with CMV-promoter-driven Luc-expressing vectors possessing unmodified, RGD-modified, or Ad5/Ad-3chimeric fibers (Ad5Luc1, Ad5RGDLuc1, and Ad5/3Luc1, respectively). After two days of incubation, the cells were lysed, and the Luc activity was measured. Du-145 and PC3 prostate cancer cell lines demonstrated significantly higher levels of transgene expression with the Ad5/Ad3—modified vector than that with Ad5Luc1. The enhancement was not significant for LnCap. In all three prostate cancer cell lines, the level of transgene expression with an RGD-modified vector tended to be slightly lower than that of Ad5Luc. Errors bars represent the standard deviation calculated from three replicates. * p < 0.05.

Journal: Viruses

Article Title: Infectivity-Enhanced, Conditionally Replicative Adenovirus for COX-2-Expressing Castration-Resistant Prostate Cancer

doi: 10.3390/v15040901

Figure Lengend Snippet: In vitro analyses. ( A ) Analysis of COX-2 RNA levels in prostate cancer cell lines. Total cellular RNA of human prostate cancer cells (LnCap, Du-145, PC3) as well as COX-2 control cells (A549 as positive and BT474 as negative) was reverse-transcribed with oligo-dT primer and amplified by PCR with COX-2—( upper panel) and GAPDH ( lower panel)-specific primers. The signal for correctly spliced cyclooxygenase-2 mRNA was detected as a 723 bp band. ( B ) Activity and selectivity of COX-2 promoter in prostate cancer cells. Human prostate cancer cells (LnCap, Du-145, PC3), COX-2—positive A549 cells, and COX-2-negative BT474 cells were infected with AdCMVLuc, AdCox2MLuc, and AdCox2LLuc. The cells were lysed and assessed for luciferase activity two days after infection. Data are shown as percentages of relative light units (RLUs) per milligram protein in relation to that of CMV-promoter-driven luciferase expression. The Cox2M promoter showed higher activity in Du-145 cells compared to the Cox2L promoter, and both had about the same activity in PC3. Minimal activity was seen in the COX-2-negative prostate cancer cell line (LnCap) and the COX-2-negative control cell line (BT474). Errors bars represent the standard deviation calculated from three replicates. * p < 0.05 ( C ) Transduction efficiency in prostate cancer cells with infectivity-enhanced Ad vectors. Human prostate cancer cells (LnCap, Du-145, PC3), and two control cell lines (A549 and BT474) were infected with CMV-promoter-driven Luc-expressing vectors possessing unmodified, RGD-modified, or Ad5/Ad-3chimeric fibers (Ad5Luc1, Ad5RGDLuc1, and Ad5/3Luc1, respectively). After two days of incubation, the cells were lysed, and the Luc activity was measured. Du-145 and PC3 prostate cancer cell lines demonstrated significantly higher levels of transgene expression with the Ad5/Ad3—modified vector than that with Ad5Luc1. The enhancement was not significant for LnCap. In all three prostate cancer cell lines, the level of transgene expression with an RGD-modified vector tended to be slightly lower than that of Ad5Luc. Errors bars represent the standard deviation calculated from three replicates. * p < 0.05.

Article Snippet: Replication-deficient adenoviral vectors (AdCox2LLuc, AdCox2MLuc, AdCMVLuc) encoding the firefly luciferase (Luc) reporter gene from pGL3-Basic (Promega, Madison, WI, USA) were constructed as previously described [ ].

Techniques: In Vitro, Control, Reverse Transcription, Amplification, Activity Assay, Infection, Luciferase, Expressing, Negative Control, Standard Deviation, Transduction, Modification, Incubation, Plasmid Preparation