acv Search Results


activin a  (Elabscience Biotechnology)
94
Elabscience Biotechnology activin a
Dose-dependent effects of ADR administration on circulating reproductive hormones: A) high-dose ADR significantly decreased LH versus control/sham, B) FSH levels remained unchanged across all groups, C) testosterone concentrations were significantly elevated following high-dose ADR treatment, D) <t>activin</t> <t>A</t> levels demonstrated a significant reduction in the high-dose ADR group, E) inhibin B levels significantly increased in the high-dose ADR group. Data are presented as mean ± SD with individual data points (n = 8 per group). One-way ANOVA with Bonferroni-adjusted omnibus α = 0.006 (0.05/8); Tukey’s HSD pairwise comparisons are reported only when the corresponding omnibus test met this adjusted threshold. *p < 0.001 vs. control; #p < 0.001 vs. sham. “ns” over a bracket denotes a nonsignificant Tukey comparison (p ≥ 0.05). Horizontal lines above bars indicate significant pairwise comparisons.
Activin A, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/activin a/product/Elabscience Biotechnology
Average 94 stars, based on 1 article reviews
activin a - by Bioz Stars, 2026-04
94/100 stars
  Buy from Supplier
2010 n a bmprib origene  (OriGene)
91
OriGene 2010 n a bmprib origene
Dose-dependent effects of ADR administration on circulating reproductive hormones: A) high-dose ADR significantly decreased LH versus control/sham, B) FSH levels remained unchanged across all groups, C) testosterone concentrations were significantly elevated following high-dose ADR treatment, D) <t>activin</t> <t>A</t> levels demonstrated a significant reduction in the high-dose ADR group, E) inhibin B levels significantly increased in the high-dose ADR group. Data are presented as mean ± SD with individual data points (n = 8 per group). One-way ANOVA with Bonferroni-adjusted omnibus α = 0.006 (0.05/8); Tukey’s HSD pairwise comparisons are reported only when the corresponding omnibus test met this adjusted threshold. *p < 0.001 vs. control; #p < 0.001 vs. sham. “ns” over a bracket denotes a nonsignificant Tukey comparison (p ≥ 0.05). Horizontal lines above bars indicate significant pairwise comparisons.
2010 N A Bmprib Origene, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/2010 n a bmprib origene/product/OriGene
Average 91 stars, based on 1 article reviews
2010 n a bmprib origene - by Bioz Stars, 2026-04
91/100 stars
  Buy from Supplier
eu 3  (DSMZ)
95
DSMZ eu 3
Dose-dependent effects of ADR administration on circulating reproductive hormones: A) high-dose ADR significantly decreased LH versus control/sham, B) FSH levels remained unchanged across all groups, C) testosterone concentrations were significantly elevated following high-dose ADR treatment, D) <t>activin</t> <t>A</t> levels demonstrated a significant reduction in the high-dose ADR group, E) inhibin B levels significantly increased in the high-dose ADR group. Data are presented as mean ± SD with individual data points (n = 8 per group). One-way ANOVA with Bonferroni-adjusted omnibus α = 0.006 (0.05/8); Tukey’s HSD pairwise comparisons are reported only when the corresponding omnibus test met this adjusted threshold. *p < 0.001 vs. control; #p < 0.001 vs. sham. “ns” over a bracket denotes a nonsignificant Tukey comparison (p ≥ 0.05). Horizontal lines above bars indicate significant pairwise comparisons.
Eu 3, supplied by DSMZ, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eu 3/product/DSMZ
Average 95 stars, based on 1 article reviews
eu 3 - by Bioz Stars, 2026-04
95/100 stars
  Buy from Supplier
acycloguanosine  (Biosynth Carbosynth)
90
Biosynth Carbosynth acycloguanosine
Primer extension from the CTNA-blocked termini by the Klenow fragment of Escherichia coli DNA polymerase I, with or without its proofreading 3′–5′ exonuclease activity (KF + or KF − , respectively, from Takara Bio, Inc., Shiga, Japan), in the ( A ) presence or ( B ) absence of dNTPs. ( A ) The 32 P-labeled oligonucleotides, 32 P-d(TCCGTTGAAGCCTGCTTT)X, where X represents no added nucleoside (OH, lanes 1–3), 2’-deoxyadenosine (lanes 4–6), <t>acyclovir</t> (ACV, lanes 7–9), abacavir (ABC, lanes 10–12), carbovir (CBV, lanes 13–15) or lamivudine ((−)3TC, lanes 16–18), were hybridized with their complementary strands, d(CTCGTCAGCTANAAAGCAGGCTTCAACGGA), where N represents A (for ABC and an oligonucleotide without CTNAs), G (for A and (−)3TC) or C (for ACV and CBV). Each substrate was incubated at 37 °C for 10 min, in the absence (lanes 1, 4, 7, 10, 13 and 16) or presence of KF − (0.1 unit, lanes 2, 5, 8, 11, 14 and 17) or KF + (0.1 unit, lanes 3, 6, 9, 12, 15 and 18), in 10 mM Tris-HCl buffer (pH 7.9) containing 50 mM NaCl, 10 mM MgCl 2 , 10 mM DTT and 100 µM dNTPs; ( B ) The 32 P-labeled substrates were incubated with KF + at 37 °C for the indicated incubation time, in the same reaction buffer without dNTPs.
Acycloguanosine, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/acycloguanosine/product/Biosynth Carbosynth
Average 90 stars, based on 1 article reviews
acycloguanosine - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
in the acv mode  (Siemens AG)
90
Siemens AG in the acv mode
Primer extension from the CTNA-blocked termini by the Klenow fragment of Escherichia coli DNA polymerase I, with or without its proofreading 3′–5′ exonuclease activity (KF + or KF − , respectively, from Takara Bio, Inc., Shiga, Japan), in the ( A ) presence or ( B ) absence of dNTPs. ( A ) The 32 P-labeled oligonucleotides, 32 P-d(TCCGTTGAAGCCTGCTTT)X, where X represents no added nucleoside (OH, lanes 1–3), 2’-deoxyadenosine (lanes 4–6), <t>acyclovir</t> (ACV, lanes 7–9), abacavir (ABC, lanes 10–12), carbovir (CBV, lanes 13–15) or lamivudine ((−)3TC, lanes 16–18), were hybridized with their complementary strands, d(CTCGTCAGCTANAAAGCAGGCTTCAACGGA), where N represents A (for ABC and an oligonucleotide without CTNAs), G (for A and (−)3TC) or C (for ACV and CBV). Each substrate was incubated at 37 °C for 10 min, in the absence (lanes 1, 4, 7, 10, 13 and 16) or presence of KF − (0.1 unit, lanes 2, 5, 8, 11, 14 and 17) or KF + (0.1 unit, lanes 3, 6, 9, 12, 15 and 18), in 10 mM Tris-HCl buffer (pH 7.9) containing 50 mM NaCl, 10 mM MgCl 2 , 10 mM DTT and 100 µM dNTPs; ( B ) The 32 P-labeled substrates were incubated with KF + at 37 °C for the indicated incubation time, in the same reaction buffer without dNTPs.
In The Acv Mode, supplied by Siemens AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/in the acv mode/product/Siemens AG
Average 90 stars, based on 1 article reviews
in the acv mode - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
autoclave apparatus acv-3167n  (Asahi Techno Glass)
90
Asahi Techno Glass autoclave apparatus acv-3167n
Primer extension from the CTNA-blocked termini by the Klenow fragment of Escherichia coli DNA polymerase I, with or without its proofreading 3′–5′ exonuclease activity (KF + or KF − , respectively, from Takara Bio, Inc., Shiga, Japan), in the ( A ) presence or ( B ) absence of dNTPs. ( A ) The 32 P-labeled oligonucleotides, 32 P-d(TCCGTTGAAGCCTGCTTT)X, where X represents no added nucleoside (OH, lanes 1–3), 2’-deoxyadenosine (lanes 4–6), <t>acyclovir</t> (ACV, lanes 7–9), abacavir (ABC, lanes 10–12), carbovir (CBV, lanes 13–15) or lamivudine ((−)3TC, lanes 16–18), were hybridized with their complementary strands, d(CTCGTCAGCTANAAAGCAGGCTTCAACGGA), where N represents A (for ABC and an oligonucleotide without CTNAs), G (for A and (−)3TC) or C (for ACV and CBV). Each substrate was incubated at 37 °C for 10 min, in the absence (lanes 1, 4, 7, 10, 13 and 16) or presence of KF − (0.1 unit, lanes 2, 5, 8, 11, 14 and 17) or KF + (0.1 unit, lanes 3, 6, 9, 12, 15 and 18), in 10 mM Tris-HCl buffer (pH 7.9) containing 50 mM NaCl, 10 mM MgCl 2 , 10 mM DTT and 100 µM dNTPs; ( B ) The 32 P-labeled substrates were incubated with KF + at 37 °C for the indicated incubation time, in the same reaction buffer without dNTPs.
Autoclave Apparatus Acv 3167n, supplied by Asahi Techno Glass, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/autoclave apparatus acv-3167n/product/Asahi Techno Glass
Average 90 stars, based on 1 article reviews
autoclave apparatus acv-3167n - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
acv  (Glaxo Smith)
90
Glaxo Smith acv
Primer extension from the CTNA-blocked termini by the Klenow fragment of Escherichia coli DNA polymerase I, with or without its proofreading 3′–5′ exonuclease activity (KF + or KF − , respectively, from Takara Bio, Inc., Shiga, Japan), in the ( A ) presence or ( B ) absence of dNTPs. ( A ) The 32 P-labeled oligonucleotides, 32 P-d(TCCGTTGAAGCCTGCTTT)X, where X represents no added nucleoside (OH, lanes 1–3), 2’-deoxyadenosine (lanes 4–6), <t>acyclovir</t> (ACV, lanes 7–9), abacavir (ABC, lanes 10–12), carbovir (CBV, lanes 13–15) or lamivudine ((−)3TC, lanes 16–18), were hybridized with their complementary strands, d(CTCGTCAGCTANAAAGCAGGCTTCAACGGA), where N represents A (for ABC and an oligonucleotide without CTNAs), G (for A and (−)3TC) or C (for ACV and CBV). Each substrate was incubated at 37 °C for 10 min, in the absence (lanes 1, 4, 7, 10, 13 and 16) or presence of KF − (0.1 unit, lanes 2, 5, 8, 11, 14 and 17) or KF + (0.1 unit, lanes 3, 6, 9, 12, 15 and 18), in 10 mM Tris-HCl buffer (pH 7.9) containing 50 mM NaCl, 10 mM MgCl 2 , 10 mM DTT and 100 µM dNTPs; ( B ) The 32 P-labeled substrates were incubated with KF + at 37 °C for the indicated incubation time, in the same reaction buffer without dNTPs.
Acv, supplied by Glaxo Smith, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/acv/product/Glaxo Smith
Average 90 stars, based on 1 article reviews
acv - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
reduced bis-acv  (Bachem)
90
Bachem reduced bis-acv
Primer extension from the CTNA-blocked termini by the Klenow fragment of Escherichia coli DNA polymerase I, with or without its proofreading 3′–5′ exonuclease activity (KF + or KF − , respectively, from Takara Bio, Inc., Shiga, Japan), in the ( A ) presence or ( B ) absence of dNTPs. ( A ) The 32 P-labeled oligonucleotides, 32 P-d(TCCGTTGAAGCCTGCTTT)X, where X represents no added nucleoside (OH, lanes 1–3), 2’-deoxyadenosine (lanes 4–6), <t>acyclovir</t> (ACV, lanes 7–9), abacavir (ABC, lanes 10–12), carbovir (CBV, lanes 13–15) or lamivudine ((−)3TC, lanes 16–18), were hybridized with their complementary strands, d(CTCGTCAGCTANAAAGCAGGCTTCAACGGA), where N represents A (for ABC and an oligonucleotide without CTNAs), G (for A and (−)3TC) or C (for ACV and CBV). Each substrate was incubated at 37 °C for 10 min, in the absence (lanes 1, 4, 7, 10, 13 and 16) or presence of KF − (0.1 unit, lanes 2, 5, 8, 11, 14 and 17) or KF + (0.1 unit, lanes 3, 6, 9, 12, 15 and 18), in 10 mM Tris-HCl buffer (pH 7.9) containing 50 mM NaCl, 10 mM MgCl 2 , 10 mM DTT and 100 µM dNTPs; ( B ) The 32 P-labeled substrates were incubated with KF + at 37 °C for the indicated incubation time, in the same reaction buffer without dNTPs.
Reduced Bis Acv, supplied by Bachem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/reduced bis-acv/product/Bachem
Average 90 stars, based on 1 article reviews
reduced bis-acv - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
acyclovir (acv)  (Beijing Solarbio Science)
90
Beijing Solarbio Science acyclovir (acv)
Primer extension from the CTNA-blocked termini by the Klenow fragment of Escherichia coli DNA polymerase I, with or without its proofreading 3′–5′ exonuclease activity (KF + or KF − , respectively, from Takara Bio, Inc., Shiga, Japan), in the ( A ) presence or ( B ) absence of dNTPs. ( A ) The 32 P-labeled oligonucleotides, 32 P-d(TCCGTTGAAGCCTGCTTT)X, where X represents no added nucleoside (OH, lanes 1–3), 2’-deoxyadenosine (lanes 4–6), <t>acyclovir</t> (ACV, lanes 7–9), abacavir (ABC, lanes 10–12), carbovir (CBV, lanes 13–15) or lamivudine ((−)3TC, lanes 16–18), were hybridized with their complementary strands, d(CTCGTCAGCTANAAAGCAGGCTTCAACGGA), where N represents A (for ABC and an oligonucleotide without CTNAs), G (for A and (−)3TC) or C (for ACV and CBV). Each substrate was incubated at 37 °C for 10 min, in the absence (lanes 1, 4, 7, 10, 13 and 16) or presence of KF − (0.1 unit, lanes 2, 5, 8, 11, 14 and 17) or KF + (0.1 unit, lanes 3, 6, 9, 12, 15 and 18), in 10 mM Tris-HCl buffer (pH 7.9) containing 50 mM NaCl, 10 mM MgCl 2 , 10 mM DTT and 100 µM dNTPs; ( B ) The 32 P-labeled substrates were incubated with KF + at 37 °C for the indicated incubation time, in the same reaction buffer without dNTPs.
Acyclovir (Acv), supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/acyclovir (acv)/product/Beijing Solarbio Science
Average 90 stars, based on 1 article reviews
acyclovir (acv) - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
liquid acv bait trap  (Contech Enterprises Inc)
90
Contech Enterprises Inc liquid acv bait trap
Primer extension from the CTNA-blocked termini by the Klenow fragment of Escherichia coli DNA polymerase I, with or without its proofreading 3′–5′ exonuclease activity (KF + or KF − , respectively, from Takara Bio, Inc., Shiga, Japan), in the ( A ) presence or ( B ) absence of dNTPs. ( A ) The 32 P-labeled oligonucleotides, 32 P-d(TCCGTTGAAGCCTGCTTT)X, where X represents no added nucleoside (OH, lanes 1–3), 2’-deoxyadenosine (lanes 4–6), <t>acyclovir</t> (ACV, lanes 7–9), abacavir (ABC, lanes 10–12), carbovir (CBV, lanes 13–15) or lamivudine ((−)3TC, lanes 16–18), were hybridized with their complementary strands, d(CTCGTCAGCTANAAAGCAGGCTTCAACGGA), where N represents A (for ABC and an oligonucleotide without CTNAs), G (for A and (−)3TC) or C (for ACV and CBV). Each substrate was incubated at 37 °C for 10 min, in the absence (lanes 1, 4, 7, 10, 13 and 16) or presence of KF − (0.1 unit, lanes 2, 5, 8, 11, 14 and 17) or KF + (0.1 unit, lanes 3, 6, 9, 12, 15 and 18), in 10 mM Tris-HCl buffer (pH 7.9) containing 50 mM NaCl, 10 mM MgCl 2 , 10 mM DTT and 100 µM dNTPs; ( B ) The 32 P-labeled substrates were incubated with KF + at 37 °C for the indicated incubation time, in the same reaction buffer without dNTPs.
Liquid Acv Bait Trap, supplied by Contech Enterprises Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/liquid acv bait trap/product/Contech Enterprises Inc
Average 90 stars, based on 1 article reviews
liquid acv bait trap - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
ultrafiltration membrane apparatus acv-3010  (Asahi Kasei Corporation)
90
Asahi Kasei Corporation ultrafiltration membrane apparatus acv-3010
Primer extension from the CTNA-blocked termini by the Klenow fragment of Escherichia coli DNA polymerase I, with or without its proofreading 3′–5′ exonuclease activity (KF + or KF − , respectively, from Takara Bio, Inc., Shiga, Japan), in the ( A ) presence or ( B ) absence of dNTPs. ( A ) The 32 P-labeled oligonucleotides, 32 P-d(TCCGTTGAAGCCTGCTTT)X, where X represents no added nucleoside (OH, lanes 1–3), 2’-deoxyadenosine (lanes 4–6), <t>acyclovir</t> (ACV, lanes 7–9), abacavir (ABC, lanes 10–12), carbovir (CBV, lanes 13–15) or lamivudine ((−)3TC, lanes 16–18), were hybridized with their complementary strands, d(CTCGTCAGCTANAAAGCAGGCTTCAACGGA), where N represents A (for ABC and an oligonucleotide without CTNAs), G (for A and (−)3TC) or C (for ACV and CBV). Each substrate was incubated at 37 °C for 10 min, in the absence (lanes 1, 4, 7, 10, 13 and 16) or presence of KF − (0.1 unit, lanes 2, 5, 8, 11, 14 and 17) or KF + (0.1 unit, lanes 3, 6, 9, 12, 15 and 18), in 10 mM Tris-HCl buffer (pH 7.9) containing 50 mM NaCl, 10 mM MgCl 2 , 10 mM DTT and 100 µM dNTPs; ( B ) The 32 P-labeled substrates were incubated with KF + at 37 °C for the indicated incubation time, in the same reaction buffer without dNTPs.
Ultrafiltration Membrane Apparatus Acv 3010, supplied by Asahi Kasei Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ultrafiltration membrane apparatus acv-3010/product/Asahi Kasei Corporation
Average 90 stars, based on 1 article reviews
ultrafiltration membrane apparatus acv-3010 - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier
acv drug  (NanoMed Pharmaceuticals)
90
NanoMed Pharmaceuticals acv drug
Primer extension from the CTNA-blocked termini by the Klenow fragment of Escherichia coli DNA polymerase I, with or without its proofreading 3′–5′ exonuclease activity (KF + or KF − , respectively, from Takara Bio, Inc., Shiga, Japan), in the ( A ) presence or ( B ) absence of dNTPs. ( A ) The 32 P-labeled oligonucleotides, 32 P-d(TCCGTTGAAGCCTGCTTT)X, where X represents no added nucleoside (OH, lanes 1–3), 2’-deoxyadenosine (lanes 4–6), <t>acyclovir</t> (ACV, lanes 7–9), abacavir (ABC, lanes 10–12), carbovir (CBV, lanes 13–15) or lamivudine ((−)3TC, lanes 16–18), were hybridized with their complementary strands, d(CTCGTCAGCTANAAAGCAGGCTTCAACGGA), where N represents A (for ABC and an oligonucleotide without CTNAs), G (for A and (−)3TC) or C (for ACV and CBV). Each substrate was incubated at 37 °C for 10 min, in the absence (lanes 1, 4, 7, 10, 13 and 16) or presence of KF − (0.1 unit, lanes 2, 5, 8, 11, 14 and 17) or KF + (0.1 unit, lanes 3, 6, 9, 12, 15 and 18), in 10 mM Tris-HCl buffer (pH 7.9) containing 50 mM NaCl, 10 mM MgCl 2 , 10 mM DTT and 100 µM dNTPs; ( B ) The 32 P-labeled substrates were incubated with KF + at 37 °C for the indicated incubation time, in the same reaction buffer without dNTPs.
Acv Drug, supplied by NanoMed Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/acv drug/product/NanoMed Pharmaceuticals
Average 90 stars, based on 1 article reviews
acv drug - by Bioz Stars, 2026-04
90/100 stars
  Buy from Supplier

Image Search Results


Dose-dependent effects of ADR administration on circulating reproductive hormones: A) high-dose ADR significantly decreased LH versus control/sham, B) FSH levels remained unchanged across all groups, C) testosterone concentrations were significantly elevated following high-dose ADR treatment, D) activin A levels demonstrated a significant reduction in the high-dose ADR group, E) inhibin B levels significantly increased in the high-dose ADR group. Data are presented as mean ± SD with individual data points (n = 8 per group). One-way ANOVA with Bonferroni-adjusted omnibus α = 0.006 (0.05/8); Tukey’s HSD pairwise comparisons are reported only when the corresponding omnibus test met this adjusted threshold. *p < 0.001 vs. control; #p < 0.001 vs. sham. “ns” over a bracket denotes a nonsignificant Tukey comparison (p ≥ 0.05). Horizontal lines above bars indicate significant pairwise comparisons.

Journal: Turkish Journal of Medical Sciences

Article Title: Neuroendocrine effects of exogenous adropin administration on the hypothalamic pituitary testicular axis in male rats

doi: 10.55730/1300-0144.6164

Figure Lengend Snippet: Dose-dependent effects of ADR administration on circulating reproductive hormones: A) high-dose ADR significantly decreased LH versus control/sham, B) FSH levels remained unchanged across all groups, C) testosterone concentrations were significantly elevated following high-dose ADR treatment, D) activin A levels demonstrated a significant reduction in the high-dose ADR group, E) inhibin B levels significantly increased in the high-dose ADR group. Data are presented as mean ± SD with individual data points (n = 8 per group). One-way ANOVA with Bonferroni-adjusted omnibus α = 0.006 (0.05/8); Tukey’s HSD pairwise comparisons are reported only when the corresponding omnibus test met this adjusted threshold. *p < 0.001 vs. control; #p < 0.001 vs. sham. “ns” over a bracket denotes a nonsignificant Tukey comparison (p ≥ 0.05). Horizontal lines above bars indicate significant pairwise comparisons.

Article Snippet: The measurements were carried out using LH (catalog no. E-EL-R0026), FSH (catalog no. E-EL-R0391), testosterone (catalog no. E-EL-R0389), inhibin B (catalog no. E-EL-R0521), and activin A (catalog no. E-EL-R0001), Elabscience, Beijing, China, commercial ELISA kits in accordance with the manufacturer’s instructions.

Techniques: Control, Comparison

Primer extension from the CTNA-blocked termini by the Klenow fragment of Escherichia coli DNA polymerase I, with or without its proofreading 3′–5′ exonuclease activity (KF + or KF − , respectively, from Takara Bio, Inc., Shiga, Japan), in the ( A ) presence or ( B ) absence of dNTPs. ( A ) The 32 P-labeled oligonucleotides, 32 P-d(TCCGTTGAAGCCTGCTTT)X, where X represents no added nucleoside (OH, lanes 1–3), 2’-deoxyadenosine (lanes 4–6), acyclovir (ACV, lanes 7–9), abacavir (ABC, lanes 10–12), carbovir (CBV, lanes 13–15) or lamivudine ((−)3TC, lanes 16–18), were hybridized with their complementary strands, d(CTCGTCAGCTANAAAGCAGGCTTCAACGGA), where N represents A (for ABC and an oligonucleotide without CTNAs), G (for A and (−)3TC) or C (for ACV and CBV). Each substrate was incubated at 37 °C for 10 min, in the absence (lanes 1, 4, 7, 10, 13 and 16) or presence of KF − (0.1 unit, lanes 2, 5, 8, 11, 14 and 17) or KF + (0.1 unit, lanes 3, 6, 9, 12, 15 and 18), in 10 mM Tris-HCl buffer (pH 7.9) containing 50 mM NaCl, 10 mM MgCl 2 , 10 mM DTT and 100 µM dNTPs; ( B ) The 32 P-labeled substrates were incubated with KF + at 37 °C for the indicated incubation time, in the same reaction buffer without dNTPs.

Journal: Molecules

Article Title: Chemical Incorporation of Chain-Terminating Nucleoside Analogs as 3′-Blocking DNA Damage and Their Removal by Human ERCC1-XPF Endonuclease

doi: 10.3390/molecules21060766

Figure Lengend Snippet: Primer extension from the CTNA-blocked termini by the Klenow fragment of Escherichia coli DNA polymerase I, with or without its proofreading 3′–5′ exonuclease activity (KF + or KF − , respectively, from Takara Bio, Inc., Shiga, Japan), in the ( A ) presence or ( B ) absence of dNTPs. ( A ) The 32 P-labeled oligonucleotides, 32 P-d(TCCGTTGAAGCCTGCTTT)X, where X represents no added nucleoside (OH, lanes 1–3), 2’-deoxyadenosine (lanes 4–6), acyclovir (ACV, lanes 7–9), abacavir (ABC, lanes 10–12), carbovir (CBV, lanes 13–15) or lamivudine ((−)3TC, lanes 16–18), were hybridized with their complementary strands, d(CTCGTCAGCTANAAAGCAGGCTTCAACGGA), where N represents A (for ABC and an oligonucleotide without CTNAs), G (for A and (−)3TC) or C (for ACV and CBV). Each substrate was incubated at 37 °C for 10 min, in the absence (lanes 1, 4, 7, 10, 13 and 16) or presence of KF − (0.1 unit, lanes 2, 5, 8, 11, 14 and 17) or KF + (0.1 unit, lanes 3, 6, 9, 12, 15 and 18), in 10 mM Tris-HCl buffer (pH 7.9) containing 50 mM NaCl, 10 mM MgCl 2 , 10 mM DTT and 100 µM dNTPs; ( B ) The 32 P-labeled substrates were incubated with KF + at 37 °C for the indicated incubation time, in the same reaction buffer without dNTPs.

Article Snippet: Acycloguanosine (acyclovir, ACV), [(1 S ,4 R )-4-(2-amino-6-(cyclopropylamino)-9 H -purin-9-yl)cyclopent-2-en-1-yl]methanol (abacavir, ABC) hemisulfate and (−)-β- l -2′,3′-dideoxy-3′-thia-cytidine (lamivudine, (−)3TC), were purchased from Sigma-Aldrich Co. (St. Louis, MO, USA), Carbosynth Ltd. (Berkshire, UK) and Wako Pure Chemical Industries, Ltd. (Osaka, Japan), respectively.

Techniques: Activity Assay, Labeling, Incubation