acs Search Results


neural progenitor cell expansion  (ATCC)
94
ATCC neural progenitor cell expansion
Neural Progenitor Cell Expansion, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hek293t  (ATCC)
96
ATCC hek293t
( A ) IF staining of small intestinal samples from Control and ISC-specific knockout (ISC-KO) mice at 1 week after TAM injection. Top 2 panels: Staining for Ki67 (gray), GFP (green), RFP (purple) and DAPI (blue). Bottom 2 panels: Staining for Lysozyme (gray), GFP (green), RFP (purple) and DAPI (blue). Scale bars, 10 μm. ( B ) Quantification of Ki67 + cells and Lysozyme + cells per crypt in ( A ) ( n = 4). Unpaired Student’s t test. ns P = 0.5512. ** P = 0.0281. ( C ) qRT-PCR analysis of Gfi1 and Sox9 mRNA levels in Vgll4 fl/fl and Vgll4 IEC-KO mice ileum samples ( n = 5). Unpaired Student’s t test. *** P = 0.0008. ns P = 0.1057. ( D ) Immunoblot analysis of GFI1, SOX9, ATOH1, and VGLL4 in ileum samples of Vgll4 fl/fl and Vgll4 IEC-KO mice. Samples were derived from the same experiment and blots were processed in parallel. ( E ) Quantification of protein levels in ( D ) ( n = 5). Unpaired Student’s t test. GFI1: ** P = 0.0018. ATOH1: ns P = 0.6427. SOX9: ns P = 0.4877. ( F ) IF staining of GFI1 (red) in Vgll4 fl/fl and Vgll4 IEC-KO mice small intestinal organoids ( n = 5). DAPI, blue. Scale bars, 30 μm. ( G ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in <t>HEK293T</t> cells. ( H ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in HEK293T cells with DNase. ( I ) Co-immunoprecipitation assay results between ATOH1 and different mutations of TEAD4 in HEK293T cells. ( J ) Co-immunoprecipitation assay results between ATOH1 and VGLL4 in HEK293T cells with or without TEAD4. ( K ) Immunoblot analysis of GST pull-down assay between purified ATOH1-ΔN, VGLL4, and TEAD4 proteins. Results are shown as mean + SD. ns no significance. .
Hek293t, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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petm  (Addgene inc)
93
Addgene inc petm
( A ) IF staining of small intestinal samples from Control and ISC-specific knockout (ISC-KO) mice at 1 week after TAM injection. Top 2 panels: Staining for Ki67 (gray), GFP (green), RFP (purple) and DAPI (blue). Bottom 2 panels: Staining for Lysozyme (gray), GFP (green), RFP (purple) and DAPI (blue). Scale bars, 10 μm. ( B ) Quantification of Ki67 + cells and Lysozyme + cells per crypt in ( A ) ( n = 4). Unpaired Student’s t test. ns P = 0.5512. ** P = 0.0281. ( C ) qRT-PCR analysis of Gfi1 and Sox9 mRNA levels in Vgll4 fl/fl and Vgll4 IEC-KO mice ileum samples ( n = 5). Unpaired Student’s t test. *** P = 0.0008. ns P = 0.1057. ( D ) Immunoblot analysis of GFI1, SOX9, ATOH1, and VGLL4 in ileum samples of Vgll4 fl/fl and Vgll4 IEC-KO mice. Samples were derived from the same experiment and blots were processed in parallel. ( E ) Quantification of protein levels in ( D ) ( n = 5). Unpaired Student’s t test. GFI1: ** P = 0.0018. ATOH1: ns P = 0.6427. SOX9: ns P = 0.4877. ( F ) IF staining of GFI1 (red) in Vgll4 fl/fl and Vgll4 IEC-KO mice small intestinal organoids ( n = 5). DAPI, blue. Scale bars, 30 μm. ( G ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in <t>HEK293T</t> cells. ( H ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in HEK293T cells with DNase. ( I ) Co-immunoprecipitation assay results between ATOH1 and different mutations of TEAD4 in HEK293T cells. ( J ) Co-immunoprecipitation assay results between ATOH1 and VGLL4 in HEK293T cells with or without TEAD4. ( K ) Immunoblot analysis of GST pull-down assay between purified ATOH1-ΔN, VGLL4, and TEAD4 proteins. Results are shown as mean + SD. ns no significance. .
Petm, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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potassium chloride  (Carolina Biological)
92
Carolina Biological potassium chloride
( A ) IF staining of small intestinal samples from Control and ISC-specific knockout (ISC-KO) mice at 1 week after TAM injection. Top 2 panels: Staining for Ki67 (gray), GFP (green), RFP (purple) and DAPI (blue). Bottom 2 panels: Staining for Lysozyme (gray), GFP (green), RFP (purple) and DAPI (blue). Scale bars, 10 μm. ( B ) Quantification of Ki67 + cells and Lysozyme + cells per crypt in ( A ) ( n = 4). Unpaired Student’s t test. ns P = 0.5512. ** P = 0.0281. ( C ) qRT-PCR analysis of Gfi1 and Sox9 mRNA levels in Vgll4 fl/fl and Vgll4 IEC-KO mice ileum samples ( n = 5). Unpaired Student’s t test. *** P = 0.0008. ns P = 0.1057. ( D ) Immunoblot analysis of GFI1, SOX9, ATOH1, and VGLL4 in ileum samples of Vgll4 fl/fl and Vgll4 IEC-KO mice. Samples were derived from the same experiment and blots were processed in parallel. ( E ) Quantification of protein levels in ( D ) ( n = 5). Unpaired Student’s t test. GFI1: ** P = 0.0018. ATOH1: ns P = 0.6427. SOX9: ns P = 0.4877. ( F ) IF staining of GFI1 (red) in Vgll4 fl/fl and Vgll4 IEC-KO mice small intestinal organoids ( n = 5). DAPI, blue. Scale bars, 30 μm. ( G ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in <t>HEK293T</t> cells. ( H ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in HEK293T cells with DNase. ( I ) Co-immunoprecipitation assay results between ATOH1 and different mutations of TEAD4 in HEK293T cells. ( J ) Co-immunoprecipitation assay results between ATOH1 and VGLL4 in HEK293T cells with or without TEAD4. ( K ) Immunoblot analysis of GST pull-down assay between purified ATOH1-ΔN, VGLL4, and TEAD4 proteins. Results are shown as mean + SD. ns no significance. .
Potassium Chloride, supplied by Carolina Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse drinking water  (Chem Impex International)
95
Chem Impex International mouse drinking water
( A ) IF staining of small intestinal samples from Control and ISC-specific knockout (ISC-KO) mice at 1 week after TAM injection. Top 2 panels: Staining for Ki67 (gray), GFP (green), RFP (purple) and DAPI (blue). Bottom 2 panels: Staining for Lysozyme (gray), GFP (green), RFP (purple) and DAPI (blue). Scale bars, 10 μm. ( B ) Quantification of Ki67 + cells and Lysozyme + cells per crypt in ( A ) ( n = 4). Unpaired Student’s t test. ns P = 0.5512. ** P = 0.0281. ( C ) qRT-PCR analysis of Gfi1 and Sox9 mRNA levels in Vgll4 fl/fl and Vgll4 IEC-KO mice ileum samples ( n = 5). Unpaired Student’s t test. *** P = 0.0008. ns P = 0.1057. ( D ) Immunoblot analysis of GFI1, SOX9, ATOH1, and VGLL4 in ileum samples of Vgll4 fl/fl and Vgll4 IEC-KO mice. Samples were derived from the same experiment and blots were processed in parallel. ( E ) Quantification of protein levels in ( D ) ( n = 5). Unpaired Student’s t test. GFI1: ** P = 0.0018. ATOH1: ns P = 0.6427. SOX9: ns P = 0.4877. ( F ) IF staining of GFI1 (red) in Vgll4 fl/fl and Vgll4 IEC-KO mice small intestinal organoids ( n = 5). DAPI, blue. Scale bars, 30 μm. ( G ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in <t>HEK293T</t> cells. ( H ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in HEK293T cells with DNase. ( I ) Co-immunoprecipitation assay results between ATOH1 and different mutations of TEAD4 in HEK293T cells. ( J ) Co-immunoprecipitation assay results between ATOH1 and VGLL4 in HEK293T cells with or without TEAD4. ( K ) Immunoblot analysis of GST pull-down assay between purified ATOH1-ΔN, VGLL4, and TEAD4 proteins. Results are shown as mean + SD. ns no significance. .
Mouse Drinking Water, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti pla2g3  (Proteintech)
93
Proteintech anti pla2g3
( A ) IF staining of small intestinal samples from Control and ISC-specific knockout (ISC-KO) mice at 1 week after TAM injection. Top 2 panels: Staining for Ki67 (gray), GFP (green), RFP (purple) and DAPI (blue). Bottom 2 panels: Staining for Lysozyme (gray), GFP (green), RFP (purple) and DAPI (blue). Scale bars, 10 μm. ( B ) Quantification of Ki67 + cells and Lysozyme + cells per crypt in ( A ) ( n = 4). Unpaired Student’s t test. ns P = 0.5512. ** P = 0.0281. ( C ) qRT-PCR analysis of Gfi1 and Sox9 mRNA levels in Vgll4 fl/fl and Vgll4 IEC-KO mice ileum samples ( n = 5). Unpaired Student’s t test. *** P = 0.0008. ns P = 0.1057. ( D ) Immunoblot analysis of GFI1, SOX9, ATOH1, and VGLL4 in ileum samples of Vgll4 fl/fl and Vgll4 IEC-KO mice. Samples were derived from the same experiment and blots were processed in parallel. ( E ) Quantification of protein levels in ( D ) ( n = 5). Unpaired Student’s t test. GFI1: ** P = 0.0018. ATOH1: ns P = 0.6427. SOX9: ns P = 0.4877. ( F ) IF staining of GFI1 (red) in Vgll4 fl/fl and Vgll4 IEC-KO mice small intestinal organoids ( n = 5). DAPI, blue. Scale bars, 30 μm. ( G ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in <t>HEK293T</t> cells. ( H ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in HEK293T cells with DNase. ( I ) Co-immunoprecipitation assay results between ATOH1 and different mutations of TEAD4 in HEK293T cells. ( J ) Co-immunoprecipitation assay results between ATOH1 and VGLL4 in HEK293T cells with or without TEAD4. ( K ) Immunoblot analysis of GST pull-down assay between purified ATOH1-ΔN, VGLL4, and TEAD4 proteins. Results are shown as mean + SD. ns no significance. .
Anti Pla2g3, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hydrochloric acid  (Carolina Biological)
91
Carolina Biological hydrochloric acid
( A ) IF staining of small intestinal samples from Control and ISC-specific knockout (ISC-KO) mice at 1 week after TAM injection. Top 2 panels: Staining for Ki67 (gray), GFP (green), RFP (purple) and DAPI (blue). Bottom 2 panels: Staining for Lysozyme (gray), GFP (green), RFP (purple) and DAPI (blue). Scale bars, 10 μm. ( B ) Quantification of Ki67 + cells and Lysozyme + cells per crypt in ( A ) ( n = 4). Unpaired Student’s t test. ns P = 0.5512. ** P = 0.0281. ( C ) qRT-PCR analysis of Gfi1 and Sox9 mRNA levels in Vgll4 fl/fl and Vgll4 IEC-KO mice ileum samples ( n = 5). Unpaired Student’s t test. *** P = 0.0008. ns P = 0.1057. ( D ) Immunoblot analysis of GFI1, SOX9, ATOH1, and VGLL4 in ileum samples of Vgll4 fl/fl and Vgll4 IEC-KO mice. Samples were derived from the same experiment and blots were processed in parallel. ( E ) Quantification of protein levels in ( D ) ( n = 5). Unpaired Student’s t test. GFI1: ** P = 0.0018. ATOH1: ns P = 0.6427. SOX9: ns P = 0.4877. ( F ) IF staining of GFI1 (red) in Vgll4 fl/fl and Vgll4 IEC-KO mice small intestinal organoids ( n = 5). DAPI, blue. Scale bars, 30 μm. ( G ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in <t>HEK293T</t> cells. ( H ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in HEK293T cells with DNase. ( I ) Co-immunoprecipitation assay results between ATOH1 and different mutations of TEAD4 in HEK293T cells. ( J ) Co-immunoprecipitation assay results between ATOH1 and VGLL4 in HEK293T cells with or without TEAD4. ( K ) Immunoblot analysis of GST pull-down assay between purified ATOH1-ΔN, VGLL4, and TEAD4 proteins. Results are shown as mean + SD. ns no significance. .
Hydrochloric Acid, supplied by Carolina Biological, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pluripotent stem cells human endothelial cells  (ATCC)
95
ATCC pluripotent stem cells human endothelial cells
( A ) IF staining of small intestinal samples from Control and ISC-specific knockout (ISC-KO) mice at 1 week after TAM injection. Top 2 panels: Staining for Ki67 (gray), GFP (green), RFP (purple) and DAPI (blue). Bottom 2 panels: Staining for Lysozyme (gray), GFP (green), RFP (purple) and DAPI (blue). Scale bars, 10 μm. ( B ) Quantification of Ki67 + cells and Lysozyme + cells per crypt in ( A ) ( n = 4). Unpaired Student’s t test. ns P = 0.5512. ** P = 0.0281. ( C ) qRT-PCR analysis of Gfi1 and Sox9 mRNA levels in Vgll4 fl/fl and Vgll4 IEC-KO mice ileum samples ( n = 5). Unpaired Student’s t test. *** P = 0.0008. ns P = 0.1057. ( D ) Immunoblot analysis of GFI1, SOX9, ATOH1, and VGLL4 in ileum samples of Vgll4 fl/fl and Vgll4 IEC-KO mice. Samples were derived from the same experiment and blots were processed in parallel. ( E ) Quantification of protein levels in ( D ) ( n = 5). Unpaired Student’s t test. GFI1: ** P = 0.0018. ATOH1: ns P = 0.6427. SOX9: ns P = 0.4877. ( F ) IF staining of GFI1 (red) in Vgll4 fl/fl and Vgll4 IEC-KO mice small intestinal organoids ( n = 5). DAPI, blue. Scale bars, 30 μm. ( G ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in <t>HEK293T</t> cells. ( H ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in HEK293T cells with DNase. ( I ) Co-immunoprecipitation assay results between ATOH1 and different mutations of TEAD4 in HEK293T cells. ( J ) Co-immunoprecipitation assay results between ATOH1 and VGLL4 in HEK293T cells with or without TEAD4. ( K ) Immunoblot analysis of GST pull-down assay between purified ATOH1-ΔN, VGLL4, and TEAD4 proteins. Results are shown as mean + SD. ns no significance. .
Pluripotent Stem Cells Human Endothelial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transfex transfection reagent  (ATCC)
99
ATCC transfex transfection reagent
( A ) IF staining of small intestinal samples from Control and ISC-specific knockout (ISC-KO) mice at 1 week after TAM injection. Top 2 panels: Staining for Ki67 (gray), GFP (green), RFP (purple) and DAPI (blue). Bottom 2 panels: Staining for Lysozyme (gray), GFP (green), RFP (purple) and DAPI (blue). Scale bars, 10 μm. ( B ) Quantification of Ki67 + cells and Lysozyme + cells per crypt in ( A ) ( n = 4). Unpaired Student’s t test. ns P = 0.5512. ** P = 0.0281. ( C ) qRT-PCR analysis of Gfi1 and Sox9 mRNA levels in Vgll4 fl/fl and Vgll4 IEC-KO mice ileum samples ( n = 5). Unpaired Student’s t test. *** P = 0.0008. ns P = 0.1057. ( D ) Immunoblot analysis of GFI1, SOX9, ATOH1, and VGLL4 in ileum samples of Vgll4 fl/fl and Vgll4 IEC-KO mice. Samples were derived from the same experiment and blots were processed in parallel. ( E ) Quantification of protein levels in ( D ) ( n = 5). Unpaired Student’s t test. GFI1: ** P = 0.0018. ATOH1: ns P = 0.6427. SOX9: ns P = 0.4877. ( F ) IF staining of GFI1 (red) in Vgll4 fl/fl and Vgll4 IEC-KO mice small intestinal organoids ( n = 5). DAPI, blue. Scale bars, 30 μm. ( G ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in <t>HEK293T</t> cells. ( H ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in HEK293T cells with DNase. ( I ) Co-immunoprecipitation assay results between ATOH1 and different mutations of TEAD4 in HEK293T cells. ( J ) Co-immunoprecipitation assay results between ATOH1 and VGLL4 in HEK293T cells with or without TEAD4. ( K ) Immunoblot analysis of GST pull-down assay between purified ATOH1-ΔN, VGLL4, and TEAD4 proteins. Results are shown as mean + SD. ns no significance. .
Transfex Transfection Reagent, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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acs 1011  (ATCC)
99
ATCC acs 1011
( A ) IF staining of small intestinal samples from Control and ISC-specific knockout (ISC-KO) mice at 1 week after TAM injection. Top 2 panels: Staining for Ki67 (gray), GFP (green), RFP (purple) and DAPI (blue). Bottom 2 panels: Staining for Lysozyme (gray), GFP (green), RFP (purple) and DAPI (blue). Scale bars, 10 μm. ( B ) Quantification of Ki67 + cells and Lysozyme + cells per crypt in ( A ) ( n = 4). Unpaired Student’s t test. ns P = 0.5512. ** P = 0.0281. ( C ) qRT-PCR analysis of Gfi1 and Sox9 mRNA levels in Vgll4 fl/fl and Vgll4 IEC-KO mice ileum samples ( n = 5). Unpaired Student’s t test. *** P = 0.0008. ns P = 0.1057. ( D ) Immunoblot analysis of GFI1, SOX9, ATOH1, and VGLL4 in ileum samples of Vgll4 fl/fl and Vgll4 IEC-KO mice. Samples were derived from the same experiment and blots were processed in parallel. ( E ) Quantification of protein levels in ( D ) ( n = 5). Unpaired Student’s t test. GFI1: ** P = 0.0018. ATOH1: ns P = 0.6427. SOX9: ns P = 0.4877. ( F ) IF staining of GFI1 (red) in Vgll4 fl/fl and Vgll4 IEC-KO mice small intestinal organoids ( n = 5). DAPI, blue. Scale bars, 30 μm. ( G ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in <t>HEK293T</t> cells. ( H ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in HEK293T cells with DNase. ( I ) Co-immunoprecipitation assay results between ATOH1 and different mutations of TEAD4 in HEK293T cells. ( J ) Co-immunoprecipitation assay results between ATOH1 and VGLL4 in HEK293T cells with or without TEAD4. ( K ) Immunoblot analysis of GST pull-down assay between purified ATOH1-ΔN, VGLL4, and TEAD4 proteins. Results are shown as mean + SD. ns no significance. .
Acs 1011, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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genex plus transfection reagent  (ATCC)
95
ATCC genex plus transfection reagent
( A ) IF staining of small intestinal samples from Control and ISC-specific knockout (ISC-KO) mice at 1 week after TAM injection. Top 2 panels: Staining for Ki67 (gray), GFP (green), RFP (purple) and DAPI (blue). Bottom 2 panels: Staining for Lysozyme (gray), GFP (green), RFP (purple) and DAPI (blue). Scale bars, 10 μm. ( B ) Quantification of Ki67 + cells and Lysozyme + cells per crypt in ( A ) ( n = 4). Unpaired Student’s t test. ns P = 0.5512. ** P = 0.0281. ( C ) qRT-PCR analysis of Gfi1 and Sox9 mRNA levels in Vgll4 fl/fl and Vgll4 IEC-KO mice ileum samples ( n = 5). Unpaired Student’s t test. *** P = 0.0008. ns P = 0.1057. ( D ) Immunoblot analysis of GFI1, SOX9, ATOH1, and VGLL4 in ileum samples of Vgll4 fl/fl and Vgll4 IEC-KO mice. Samples were derived from the same experiment and blots were processed in parallel. ( E ) Quantification of protein levels in ( D ) ( n = 5). Unpaired Student’s t test. GFI1: ** P = 0.0018. ATOH1: ns P = 0.6427. SOX9: ns P = 0.4877. ( F ) IF staining of GFI1 (red) in Vgll4 fl/fl and Vgll4 IEC-KO mice small intestinal organoids ( n = 5). DAPI, blue. Scale bars, 30 μm. ( G ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in <t>HEK293T</t> cells. ( H ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in HEK293T cells with DNase. ( I ) Co-immunoprecipitation assay results between ATOH1 and different mutations of TEAD4 in HEK293T cells. ( J ) Co-immunoprecipitation assay results between ATOH1 and VGLL4 in HEK293T cells with or without TEAD4. ( K ) Immunoblot analysis of GST pull-down assay between purified ATOH1-ΔN, VGLL4, and TEAD4 proteins. Results are shown as mean + SD. ns no significance. .
Genex Plus Transfection Reagent, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


( A ) IF staining of small intestinal samples from Control and ISC-specific knockout (ISC-KO) mice at 1 week after TAM injection. Top 2 panels: Staining for Ki67 (gray), GFP (green), RFP (purple) and DAPI (blue). Bottom 2 panels: Staining for Lysozyme (gray), GFP (green), RFP (purple) and DAPI (blue). Scale bars, 10 μm. ( B ) Quantification of Ki67 + cells and Lysozyme + cells per crypt in ( A ) ( n = 4). Unpaired Student’s t test. ns P = 0.5512. ** P = 0.0281. ( C ) qRT-PCR analysis of Gfi1 and Sox9 mRNA levels in Vgll4 fl/fl and Vgll4 IEC-KO mice ileum samples ( n = 5). Unpaired Student’s t test. *** P = 0.0008. ns P = 0.1057. ( D ) Immunoblot analysis of GFI1, SOX9, ATOH1, and VGLL4 in ileum samples of Vgll4 fl/fl and Vgll4 IEC-KO mice. Samples were derived from the same experiment and blots were processed in parallel. ( E ) Quantification of protein levels in ( D ) ( n = 5). Unpaired Student’s t test. GFI1: ** P = 0.0018. ATOH1: ns P = 0.6427. SOX9: ns P = 0.4877. ( F ) IF staining of GFI1 (red) in Vgll4 fl/fl and Vgll4 IEC-KO mice small intestinal organoids ( n = 5). DAPI, blue. Scale bars, 30 μm. ( G ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in HEK293T cells. ( H ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in HEK293T cells with DNase. ( I ) Co-immunoprecipitation assay results between ATOH1 and different mutations of TEAD4 in HEK293T cells. ( J ) Co-immunoprecipitation assay results between ATOH1 and VGLL4 in HEK293T cells with or without TEAD4. ( K ) Immunoblot analysis of GST pull-down assay between purified ATOH1-ΔN, VGLL4, and TEAD4 proteins. Results are shown as mean + SD. ns no significance. .

Journal: EMBO Reports

Article Title: VGLL4 modulates Paneth cells and sustains intestinal homeostasis

doi: 10.1038/s44319-026-00699-3

Figure Lengend Snippet: ( A ) IF staining of small intestinal samples from Control and ISC-specific knockout (ISC-KO) mice at 1 week after TAM injection. Top 2 panels: Staining for Ki67 (gray), GFP (green), RFP (purple) and DAPI (blue). Bottom 2 panels: Staining for Lysozyme (gray), GFP (green), RFP (purple) and DAPI (blue). Scale bars, 10 μm. ( B ) Quantification of Ki67 + cells and Lysozyme + cells per crypt in ( A ) ( n = 4). Unpaired Student’s t test. ns P = 0.5512. ** P = 0.0281. ( C ) qRT-PCR analysis of Gfi1 and Sox9 mRNA levels in Vgll4 fl/fl and Vgll4 IEC-KO mice ileum samples ( n = 5). Unpaired Student’s t test. *** P = 0.0008. ns P = 0.1057. ( D ) Immunoblot analysis of GFI1, SOX9, ATOH1, and VGLL4 in ileum samples of Vgll4 fl/fl and Vgll4 IEC-KO mice. Samples were derived from the same experiment and blots were processed in parallel. ( E ) Quantification of protein levels in ( D ) ( n = 5). Unpaired Student’s t test. GFI1: ** P = 0.0018. ATOH1: ns P = 0.6427. SOX9: ns P = 0.4877. ( F ) IF staining of GFI1 (red) in Vgll4 fl/fl and Vgll4 IEC-KO mice small intestinal organoids ( n = 5). DAPI, blue. Scale bars, 30 μm. ( G ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in HEK293T cells. ( H ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 in HEK293T cells with DNase. ( I ) Co-immunoprecipitation assay results between ATOH1 and different mutations of TEAD4 in HEK293T cells. ( J ) Co-immunoprecipitation assay results between ATOH1 and VGLL4 in HEK293T cells with or without TEAD4. ( K ) Immunoblot analysis of GST pull-down assay between purified ATOH1-ΔN, VGLL4, and TEAD4 proteins. Results are shown as mean + SD. ns no significance. .

Article Snippet: HEK293T , ATCC , ACS-4500.

Techniques: Staining, Control, Knock-Out, Injection, Quantitative RT-PCR, Western Blot, Derivative Assay, Co-Immunoprecipitation Assay, Pull Down Assay, Purification

( A ) qRT-PCR analysis of Math1 from Vgll4 fl/fl and Vgll4 IEC-KO mice ileum samples ( n = 5). Unpaired Student’s t test. ns P = 0.7009. ( B ) Co-immunoprecipitation assay results between ATOH1 and VGLL4 in HEK293T cells. ( C ) Schematic illustration of TEAD4 and its short forms. ( D ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 short forms in HEK293T cells. The black arrow indicates IgG heavy chain. ( E ) Co-immunoprecipitation assay results between ATOH1 and TEAD4-N and TEA domain short forms in HEK293T cells. ( F ) Schematic illustration of ATOH1 and its short forms. ( G ) Co-immunoprecipitation assay results between ATOH1 short forms and TEAD4 in HEK293T cells. ( H ) Coomassie brilliant blue staining of the GST-pulldown assay between ATOH1-ΔN, VGLL4, and TEAD4. ( I ) FLAG antibody pulldown assay results between VGLL4-TEAD4-ATOH1 complex in HEK293T cells overexpressing FLAG-VGLL4, MYC-ATOH1 and HA-TEAD4. ( J ) ChIP-qPCR analysis of TEAD4 enrichment at the GFI1 promoter in HEK293T cells overexpressing FLAG-TEAD4. Unpaired Student’s t test. **** P < 0.0001. Three biological replicates per group. ( K ) ChIP-qPCR analysis of TEAD4 at the GFI1 promoter in 293T cells with or without VGLL4 overexpression. Unpaired Student’s t test. **** P < 0.0001. Left *** P = 0.0007. Right *** P = 0.0003. Three biological replicates per group. ( L ) ChIP-qPCR analysis of ATOH1 at the GFI1 promoter in HEK293T cells with or without VGLL4 overexpression. Unpaired Student’s t test. Left *** P = 0.0003. Right *** P = 0.0007. Three biological replicates per group. ( M ) A model of GFI1 luciferase construction strategy. Red arrow: TBS, TEAD4 binding sequence. Black arrow: ABS, ATOH1 binding sequence. RR: regulatory region. ( N ) GFI1 Luc reporter activity in HCoEpiC cells transfected with ATOH1, VGLL4, and TEAD4. One-way ANOVA. **** P < 0.0001. Three biological replicates per group. ( O ) GFI1 Luc reporter activity in HCoEpiC cells transfected with ATOH1, TEAD4 wild-type or mutant form, VGLL4 wild-type or mutant form. One-way ANOVA. **** P < 0.0001. Three biological replicates per group. Results are shown as mean + SD. ns no significance.

Journal: EMBO Reports

Article Title: VGLL4 modulates Paneth cells and sustains intestinal homeostasis

doi: 10.1038/s44319-026-00699-3

Figure Lengend Snippet: ( A ) qRT-PCR analysis of Math1 from Vgll4 fl/fl and Vgll4 IEC-KO mice ileum samples ( n = 5). Unpaired Student’s t test. ns P = 0.7009. ( B ) Co-immunoprecipitation assay results between ATOH1 and VGLL4 in HEK293T cells. ( C ) Schematic illustration of TEAD4 and its short forms. ( D ) Co-immunoprecipitation assay results between ATOH1 and TEAD4 short forms in HEK293T cells. The black arrow indicates IgG heavy chain. ( E ) Co-immunoprecipitation assay results between ATOH1 and TEAD4-N and TEA domain short forms in HEK293T cells. ( F ) Schematic illustration of ATOH1 and its short forms. ( G ) Co-immunoprecipitation assay results between ATOH1 short forms and TEAD4 in HEK293T cells. ( H ) Coomassie brilliant blue staining of the GST-pulldown assay between ATOH1-ΔN, VGLL4, and TEAD4. ( I ) FLAG antibody pulldown assay results between VGLL4-TEAD4-ATOH1 complex in HEK293T cells overexpressing FLAG-VGLL4, MYC-ATOH1 and HA-TEAD4. ( J ) ChIP-qPCR analysis of TEAD4 enrichment at the GFI1 promoter in HEK293T cells overexpressing FLAG-TEAD4. Unpaired Student’s t test. **** P < 0.0001. Three biological replicates per group. ( K ) ChIP-qPCR analysis of TEAD4 at the GFI1 promoter in 293T cells with or without VGLL4 overexpression. Unpaired Student’s t test. **** P < 0.0001. Left *** P = 0.0007. Right *** P = 0.0003. Three biological replicates per group. ( L ) ChIP-qPCR analysis of ATOH1 at the GFI1 promoter in HEK293T cells with or without VGLL4 overexpression. Unpaired Student’s t test. Left *** P = 0.0003. Right *** P = 0.0007. Three biological replicates per group. ( M ) A model of GFI1 luciferase construction strategy. Red arrow: TBS, TEAD4 binding sequence. Black arrow: ABS, ATOH1 binding sequence. RR: regulatory region. ( N ) GFI1 Luc reporter activity in HCoEpiC cells transfected with ATOH1, VGLL4, and TEAD4. One-way ANOVA. **** P < 0.0001. Three biological replicates per group. ( O ) GFI1 Luc reporter activity in HCoEpiC cells transfected with ATOH1, TEAD4 wild-type or mutant form, VGLL4 wild-type or mutant form. One-way ANOVA. **** P < 0.0001. Three biological replicates per group. Results are shown as mean + SD. ns no significance.

Article Snippet: HEK293T , ATCC , ACS-4500.

Techniques: Quantitative RT-PCR, Co-Immunoprecipitation Assay, Staining, GST Pulldown Assay, ChIP-qPCR, Over Expression, Luciferase, Binding Assay, Sequencing, Activity Assay, Transfection, Mutagenesis

( A ) Heatmap analysis of defensin expression with RNA-seq data ( n = 4). ( B ) ChIP-qPCR analysis of TCF4 enrichment at the DEFA5 promoter in SW620 cells. Unpaired Student’s t test. *** P = 0.0008. Three biological replicates per group. ( C ) ChIP-qPCR analysis of TEAD4 enrichment at the DEFA5 promoter in SW620 cells. Unpaired Student’s t test. *** P = 0.0003. Three biological replicates per group. ( D ) Luciferase reporter activity driven by DEFA5 promoter in HEK293T cells transfected with indicated plasmids. One-way ANOVA. **** P < 0.0001. Three biological replicates per group. ( E ) Luciferase reporter activity driven by DEFA5 promoter in HEK293T cells transfected with TCF4, VGLL4 and TEAD4 wild-type or TEAD4-TEA short form. One-way ANOVA. **** P < 0.0001. *** P = 0.0004. Three biological replicates per group. ( F ) Luciferase reporter activity driven by DEFA5 promoter in HEK293T cells transfected with TCF4, TEAD4 and VGLL4 wild-type or VGLL4-HF4A short form. One-way ANOVA. **** P < 0.0001. Three biological replicates per group. ( G ) Luciferase reporter activity driven by DEFA5 promoter in HCoEpiC cells transfected with indicated plasmids. One-way ANOVA. **** P < 0.0001. ** P = 0.0025. Three biological replicates per group. ( H ) Luciferase reporter activity driven by DEFA5 promoter in HCoEpiC cells transfected with TCF4, TEAD4 wild-type or TEAD4-TEA short form, and VGLL4 wild-type or VGLL4-HF4A short form. One-way ANOVA. **** P < 0.0001. Three biological replicates per group. Results are shown as mean + SD.

Journal: EMBO Reports

Article Title: VGLL4 modulates Paneth cells and sustains intestinal homeostasis

doi: 10.1038/s44319-026-00699-3

Figure Lengend Snippet: ( A ) Heatmap analysis of defensin expression with RNA-seq data ( n = 4). ( B ) ChIP-qPCR analysis of TCF4 enrichment at the DEFA5 promoter in SW620 cells. Unpaired Student’s t test. *** P = 0.0008. Three biological replicates per group. ( C ) ChIP-qPCR analysis of TEAD4 enrichment at the DEFA5 promoter in SW620 cells. Unpaired Student’s t test. *** P = 0.0003. Three biological replicates per group. ( D ) Luciferase reporter activity driven by DEFA5 promoter in HEK293T cells transfected with indicated plasmids. One-way ANOVA. **** P < 0.0001. Three biological replicates per group. ( E ) Luciferase reporter activity driven by DEFA5 promoter in HEK293T cells transfected with TCF4, VGLL4 and TEAD4 wild-type or TEAD4-TEA short form. One-way ANOVA. **** P < 0.0001. *** P = 0.0004. Three biological replicates per group. ( F ) Luciferase reporter activity driven by DEFA5 promoter in HEK293T cells transfected with TCF4, TEAD4 and VGLL4 wild-type or VGLL4-HF4A short form. One-way ANOVA. **** P < 0.0001. Three biological replicates per group. ( G ) Luciferase reporter activity driven by DEFA5 promoter in HCoEpiC cells transfected with indicated plasmids. One-way ANOVA. **** P < 0.0001. ** P = 0.0025. Three biological replicates per group. ( H ) Luciferase reporter activity driven by DEFA5 promoter in HCoEpiC cells transfected with TCF4, TEAD4 wild-type or TEAD4-TEA short form, and VGLL4 wild-type or VGLL4-HF4A short form. One-way ANOVA. **** P < 0.0001. Three biological replicates per group. Results are shown as mean + SD.

Article Snippet: HEK293T , ATCC , ACS-4500.

Techniques: Expressing, RNA Sequencing, ChIP-qPCR, Luciferase, Activity Assay, Transfection