Journal: Journal of cellular and molecular medicine

Article Title: DDR1 Targeting HOXA6 Facilitates Bladder Cancer Progression via Inhibiting Ferroptosis.

doi: 10.1111/jcmm.70410

Figure Lengend Snippet: FIGURE 3 | DDR1 inhibits ferroptosis of BC cells. (A) CCK-8 assay of TCCSUP cells treated with erastin, apoptosis inhibitor ZVAD-FMK, necro- ptosis inhibitor necrosulfonamide or ferroptosis inhibitor ferrostatin-1 combined with NC and OE-DDR1. (B) CCK-8 assay of T24 cells treated with erastin, apoptosis inhibitor ZVAD-FMK, necroptosis inhibitor necrosulfonamide or ferroptosis inhibitor ferrostatin-1 combined with NC and OE- DDR1. (C) Levels of glutathione (GSH), malondialdehyde (MDA) and Fe2+ in TCCSUP cells. (D) Levels of GSH, MDA and Fe2+ in T24 cells. (E) RT- qPCR analysis of glutathione peroxidase 4 (GPX4), solute carrier family 7 member 11 (SLC7A11) and acyl-CoA synthetase long chain family member 4 (ACSL4) expression in TCCSUP cells. (F) RT-qPCR analysis of GPX4, SLC7A11 and ACSL4 expression in T24 cells. (G) WB analysis and quantifi- cation of SLC7A11, zinc finger E-box binding homeobox 1 (ZEB1), E-cadherin, ACSL4, GPX4, yes-associated protein (YAP), p-YAP, neurofibromin 2 (NF2), and p-NF2 expression in TCCSUP and T24 cells. N = 3. *p < 0.05 versus control group. #p < 0.05 versus NC + erastin or si-NC + erastin group.

Article Snippet: The membranes were blocked with 5% skim milk at 37°C for 1 h and then incubated using primary antibodies against DDR1 (#SC- 374618, 1:1000, Santa Cruz Biotechnology, Dallas, USA), solute carrier family 7 member 11 (SLC7A11, #Ab175186, 1:1000, Abcam, Cambridge, UK), yes- associated protein (YAP, #13584- 1- AP, 1:2000, Proteintech, Rosemont, USA), p- YAP (#29018- 1- AP, 1:2000, Proteintech), acyl- CoA synthetase long chain family member 4 (ACSL4, #Ab155282, 1:10000, Abcam), zinc finger E- box binding homeobox 1 (ZEB1, #Sc- 515797, 1:1000, Santa Cruz Biotechnology), Ecadherin (#20874- 1- AP, 1:20000, Proteintech), glutathione peroxidase 4 (GPX4, #Ab125066, 1:1000, Abcam), neurofibromin 2 (NF2, #21686- 1- AP, 1:2000, Proteintech), p- NF2 (#28851- 1- AP, 1:1000, Proteintech), HOXA6 (#18210- 1- AP, 1:1000, Proteintech) and β- actin (#66009–1- Ig, 1:20000, Proteintech) overnight at 4°C.

Techniques: CCK-8 Assay, Quantitative RT-PCR, Expressing, Binding Assay, Control

Journal: Journal of cellular and molecular medicine

Article Title: DDR1 Targeting HOXA6 Facilitates Bladder Cancer Progression via Inhibiting Ferroptosis.

doi: 10.1111/jcmm.70410

Figure Lengend Snippet: FIGURE 8 | Effect of DDR1 on the levels of proteins involved in ferroptosis, EMT and NF2–YAP pathway in T24 xenografts. Representative IHC staining and quantification of GPX4, SLC7A11, ACSL4, ZEB1, E-cadherin, p-YAP and p-NF2 in T24 xenograft group tumours. N = 3. *p < 0.05 versus control group. *p < 0.05 versus control group. #p < 0.05 versus erastin group. $p < 0.05 versus OE-DDR1 + erastin group.

Article Snippet: The membranes were blocked with 5% skim milk at 37°C for 1 h and then incubated using primary antibodies against DDR1 (#SC- 374618, 1:1000, Santa Cruz Biotechnology, Dallas, USA), solute carrier family 7 member 11 (SLC7A11, #Ab175186, 1:1000, Abcam, Cambridge, UK), yes- associated protein (YAP, #13584- 1- AP, 1:2000, Proteintech, Rosemont, USA), p- YAP (#29018- 1- AP, 1:2000, Proteintech), acyl- CoA synthetase long chain family member 4 (ACSL4, #Ab155282, 1:10000, Abcam), zinc finger E- box binding homeobox 1 (ZEB1, #Sc- 515797, 1:1000, Santa Cruz Biotechnology), Ecadherin (#20874- 1- AP, 1:20000, Proteintech), glutathione peroxidase 4 (GPX4, #Ab125066, 1:1000, Abcam), neurofibromin 2 (NF2, #21686- 1- AP, 1:2000, Proteintech), p- NF2 (#28851- 1- AP, 1:1000, Proteintech), HOXA6 (#18210- 1- AP, 1:1000, Proteintech) and β- actin (#66009–1- Ig, 1:20000, Proteintech) overnight at 4°C.

Techniques: Immunohistochemistry, Control