Journal: Cell systems

Article Title: Dissecting RAF inhibitor resistance by structure-based modeling reveals ways to overcome oncogenic RAS signaling

doi: 10.1016/j.cels.2018.06.002

Figure Lengend Snippet: Key resources table

Article Snippet: Reagent or resource Source Identifier Antibodies Anti-MAP Kinase (ERK-1, ERK-2) antibody produced in rabbit Sigma-Aldrich Cat. No. M5670 Monoclonal Anti-MAP Kinase, Activated (Diphosphorylated ERK-1&2) antibody produced in mouse Sigma-Aldrich Cat. No. M8159 Anti-rabbit IgG, HRP-linked Antibody Cell Signalling Technologies Cat. No. 7074 Anti-mouse IgG, HRP-linked Antibody Cell Signalling Technologies Cat. No. 7076 Chemicals, Peptides, and Recombinant Proteins Vemurafenib (PLX4032) Selleckchem Cat. No. S1267 Dabrafenib (GSK2118436) Selleckchem Cat. No.S2807 TAK-632 Selleckchem Cat. No.S7291 Trametinib (GSK1120212) Selleckchem Cat. No.S2673 Sorafenib tosylate Axon Medchem Axon 1397 SB-590885 R&D Systems 2650/10 B0R (2,6-difluoro-N-(3-methoxy-2H-pyrazolo[3,4-b]pyridin-5-yl)-3-[(propylsulfonyl) amino]benzamide) Genentech, ( Wenglowsky et al., 2011 ) B0R Critical Commercial Assays pMEK assay ProtATonce P-MP2K1-A01 pERK assay ProtATonce P-MK03-A01 GAPDH assay ProtATonce P-GAPDH-A01 Assay buffer ProtATonce PPK40-A01 Lysis buffer ProtATonce PLY40-A01 Phospho(Ser217/221)/Total MEK1/2 Assay Whole Cell Lysate Kit Meso Scale Discovery K15129D Phospho(Thr202/Tyr204; Thr185/Tyr187)/Total ERK1/2 Assay Whole Cell Lysate Kit Meso Scale Discovery K15107D PierceTM BCA Protein Assay Kit ThermoFisher Scientific/Pierce Cat. No. 23225 CellTiter 96® Aqueous One Solution Cell Proliferation Assay (MTS) Promega Cat. No. G3581 Deposited Data Dose-response MESOSCALE data of MEK and ERK signaling in cells treated with RAF inhibitors This study Data S4.xlsx Dose-response Luminex data of MEK and ERK signaling in cells treated with RAF inhibitors This study Data S4.xlsx Experimental Models: Cell Lines SKMEL2 ATCC HTB-68 A375 ATCC CRL-1619 MEL-JUSO DSMZ ACC 74 M249 parental and resistant Antoni Ribas ( Nazarian et al., 2010 ) Software and Algorithms Calculation number of colonies Clono-Counter ( Niyazi et al., 2007 ) Simulation of rule-based models BioNetGen http://www.csb.pitt.edu/Faculty/Faeder/?page_id=409 Spreadsheet for calculation of apparent dissociation constants.

Techniques: Produced, Recombinant, Lysis, Bicinchoninic Acid Protein Assay, Proliferation Assay, Luminex, Software

Journal: Cell reports

Article Title: A MYC-controlled redox switch protects B lymphoma cells from EGR1-dependent apoptosis.

doi: 10.1016/j.celrep.2023.112961

Figure Lengend Snippet: Figure 1. N-acetylcysteine and vitamin C selectively kill human B lymphoma cell lines and inhibit tumor growth in vivo (A) Viability of human B lymphoma cell lines following 48 h incubation with N-acetylcysteine (NAC; left) and vitamin C (VitC; right) as judged by PrestoBlue-based assays. (B) Number of cells remaining after incubation with NAC and VitC for 72 h. (C) Number of Ramos (left) and LY1 (right) cells remaining after incubation with lower doses of NAC, VitC, and both compounds for 48 h. (D and E) Tumor growth in NSG mice injected s.c. with 107 Ramos (left) and LY1 (right) cells. When tumors had developed, the mice were injected i.p. with PBS (Ctrl, n = 11) and higher doses of NAC (n = 7) and VitC (n = 7) (D) and lower doses of NAC (n = 13), VitC (n = 13), and both NAC and VitC (n = 7) (E) five times/week; tumors were measured three times/week. (F) Tumor growth in NSG mice engrafted subcutaneously with 107 IM9 cells. When tumors had developed, the mice were injected i.p. with PBS (Ctrl, n = 5), NAC (n = 5), and VitC (n = 5) five times/week. *p < 0.05; **p < 0.01; ***p < 0.005; ****p < 0.001.

Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Dextran solution Pharmacia Cat#Dextran T-500 NH4Cl StemCell Cat#07800 FxCycle PI/RNase Staining Solution Invitrogen Cat#1985233 MethoCult medium Stemcell Technologies Cat#03630 Chemicals, peptides, and recombinant proteins N-acetylcysteine Sigma Cat#A7250 Vitamin C Sigma Cat#A5960 L-Glutathione Sigma Cat#G4251 L-Cysteine Sigma Cat#C8755 I-BET762 (SML1272-5MG), Sigma Cat#SML1272-5MG L-Buthionine-sulfoximine Sigma Cat#B2515 Z-VAD-FMK Sigma Cat#V116-2MG Doxycycline Sigma-Aldrich Cat#D1822 Non-Essential Amino Acids ThermoFisher Cat#11140050 PrestoBlue Cell Viability Reagent ThermoFisher Cat#A13262 Polybrene Sigma-Aldrich Cat#107689-10G Urea Sigma-Aldrich Cat#U0631 Critical commercial assays GSH/GSSG-Glo assay Promega Cat#V6611 Alexa Fluor 488 Annexin V ThermoFisher Cat#A13201 Propidium iodide (PI) Biolegend Cat#421301 Complete protease inhibitor cocktail ThermoFisher Cat#78430 Dynabeads Protein G Immunoprecipitation Kit ThermoFisher Cat#10007D ROS-Glo-H2O2 assay Promega Cat#G8820 H2DCFDA (H2-DCF, DCF) ThermoFisher Cat#D399 Deposited data Raw and analyzed data This paper GSE199843; https://www.ncbi.nlm.nih.gov/geo/ query/acc.cgi?acc=GSE199843 Experimental models: Cell lines IM9 ATCC Cat#CCL-159 BL2 DSMZ Cat#ACC-625 L428, LY1, LY3, Karpas-422, DB, Daudi, and Ramos Gift from Dr. S. Okret, Karolinska Institutet N/A P493-6, and Jurkat Gift from Dr. Y. Bryceson, Karolinska Institutet N/A TALL1 DSMZ Cat#ACC 521 Experimental models: Organisms/strains lMYC mice C57BL/6–129/Sv mixed genetic background.

Techniques: In Vivo, Incubation, Injection