Journal: Bioengineering

Article Title: Controlled-Release Hydrogel Microspheres to Deliver Multipotent Stem Cells for Treatment of Knee Osteoarthritis

doi: 10.3390/bioengineering10111315

Figure Lengend Snippet: Assay identification numbers used in the study.

Article Snippet: ACAN , Rn00573424_m1.

Techniques:

Journal: Bioengineering

Article Title: Controlled-Release Hydrogel Microspheres to Deliver Multipotent Stem Cells for Treatment of Knee Osteoarthritis

doi: 10.3390/bioengineering10111315

Figure Lengend Snippet: Assay identification numbers and C t values for unencapsulated and encapsulated MSCs.

Article Snippet: ACAN , Rn00573424_m1.

Techniques:

Journal: Tissue Engineering. Part C, Methods

Article Title: A Novel Bioreactor System Capable of Simulating the In Vivo Conditions of Synovial Joints

doi: 10.1089/ten.tec.2020.0161

Figure Lengend Snippet: Engineering of cartilage and culture of articular cartilage explants. Chondrocytes were seeded on collagen scaffolds and cultured for 14 days in a standard cell culture incubator using differentiation medium supplemented with transforming growth factor β1. The cell-seeded scaffolds were mounted on CaP substrate and cultured for further 14 days either as controls in plastic dishes ( top figure ) or within the PRRS in the sample holders by application of 5 N dynamic compression ( bottom figure ). Histological sections of the cell-seeded scaffolds were stained with Safranin-O /Fast Green to highlight the deposition of the glycosaminoglycans. (a) Histological sections of the tissue-engineered cartilage tissues in control and PRRS cultures. (b) Depicts detailed view from regions of the cell-seeded scaffolds highlighted in squares . Cartilage formation and maintenance was possible in both conditions. A cartilaginous tissue with chondrocytes located in lacunae and visually a thicker cartilage layer formed in the samples cultured in the PRRS. Dynamic compression and shear stress upregulated the levels of transcripts encoding the cartilage-specific COL2 and ACAN, whereas levels of transcripts encoding COL1 were downregulated (c) . Moreover, dynamic compression or shear stress retained the tissue integrity, as observed histologically (d) .

Article Snippet: Total RNA was reverse transcribed using murine leukemia virus (MLV) reverse transcriptase (Promega, Dübendorf, Switzerland), and quantitative PCR was performed on an ABI 7500 sequence detection system (Life Technologies) with the following Taqman Assays-on Demand: collagen type II, alpha 1 chain (COL2A1, Bt03251861_m1), collagen type I, alpha 1 chain (COL1A1, Bt03225322_m1), and aggrecan (ACAN, Bt03212186_m1).

Techniques: Cell Culture, Staining, Control, Shear