abx-ma1 Search Results


abx-ma1  (PDL BioPharma)
90
PDL BioPharma abx-ma1
Abx Ma1, supplied by PDL BioPharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/abx-ma1/abx+ma1/us08822652-928-80-75
Average 90 stars, based on 1 article reviews
abx-ma1 - by Bioz Stars, 2026-10
90/100 stars
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anti-cd146 human mab, abx-ma1  (ProteoGenix)
90
ProteoGenix anti-cd146 human mab, abx-ma1
mOI-3, chOI-3 and <t>ABX-MA1</t> bind to CD146 expressed on human mesothelioma cancer cell lines. ( A ) and ( C ) Representative flow cytometry histograms comparing the binding of increasing concentrations, 0.001–10 µg of mOI-3 Alexa 488 and chOI-3 Alexa 488 to ( A ) MSTO-211H, and ( C ) NCI-H226 cells for 30 min at 4 °C. Unstained cells, mouse, and human isotype controls were used as negative controls. ( B ) and ( D ) relative median fluorescence intensity (rMFI) for all samples was calculated based on rMFI of isotype control cells for MSTO-211H and NCI-H226, respectively. A one-site binding model (Y = B max × X / (KD + X)) was used to analyze the data plotted as a hyperbolic curve (GraphPad Prism, La Jolla, USA). This analysis aimed to determine the apparent equilibrium dissociation constant (K D APP ) specific to cancer cell lines. For MSTO-211H, K D APP was 3.3 ± 0.3 nM and 9.2 ± 1.4 nM for chOI-3 and mOI-3 respectively. For NCI-H226, K D APP was 5.9 ± 1.3 nM and 11.4 ± 4.0 nM for chOI-3 and mOI-3 respectively. ( E ) MSTO-211H, and ( F ) NCI-H226 cells were stained with human unlabeled antibody ABX-MA1 and chOI-3 for 30 min at 4 °C. Following the incubation, goat anti-human Alexa 488 antibody was used for 30 min 4 °C. In all experiments, only MitoTracker Red CMXRos and DRAQ5 double positive cells were included in the analysis. Samples were collected using CytoFlex. Data are representative of at least two or three independent experiments.
Anti Cd146 Human Mab, Abx Ma1, supplied by ProteoGenix, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/abx-ma1/anti+cd146+human+mab++abx+ma1/pmc11522520-236-3-7
Average 90 stars, based on 1 article reviews
anti-cd146 human mab, abx-ma1 - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
abx-ma1  (TargetMol)
N/A
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Image Search Results


mOI-3, chOI-3 and ABX-MA1 bind to CD146 expressed on human mesothelioma cancer cell lines. ( A ) and ( C ) Representative flow cytometry histograms comparing the binding of increasing concentrations, 0.001–10 µg of mOI-3 Alexa 488 and chOI-3 Alexa 488 to ( A ) MSTO-211H, and ( C ) NCI-H226 cells for 30 min at 4 °C. Unstained cells, mouse, and human isotype controls were used as negative controls. ( B ) and ( D ) relative median fluorescence intensity (rMFI) for all samples was calculated based on rMFI of isotype control cells for MSTO-211H and NCI-H226, respectively. A one-site binding model (Y = B max × X / (KD + X)) was used to analyze the data plotted as a hyperbolic curve (GraphPad Prism, La Jolla, USA). This analysis aimed to determine the apparent equilibrium dissociation constant (K D APP ) specific to cancer cell lines. For MSTO-211H, K D APP was 3.3 ± 0.3 nM and 9.2 ± 1.4 nM for chOI-3 and mOI-3 respectively. For NCI-H226, K D APP was 5.9 ± 1.3 nM and 11.4 ± 4.0 nM for chOI-3 and mOI-3 respectively. ( E ) MSTO-211H, and ( F ) NCI-H226 cells were stained with human unlabeled antibody ABX-MA1 and chOI-3 for 30 min at 4 °C. Following the incubation, goat anti-human Alexa 488 antibody was used for 30 min 4 °C. In all experiments, only MitoTracker Red CMXRos and DRAQ5 double positive cells were included in the analysis. Samples were collected using CytoFlex. Data are representative of at least two or three independent experiments.

Journal: Scientific Reports

Article Title: Antigen targeting and anti-tumor activity of a novel anti-CD146 212 Pb internalizing alpha-radioimmunoconjugate against malignant peritoneal mesothelioma

doi: 10.1038/s41598-024-76778-z

Figure Lengend Snippet: mOI-3, chOI-3 and ABX-MA1 bind to CD146 expressed on human mesothelioma cancer cell lines. ( A ) and ( C ) Representative flow cytometry histograms comparing the binding of increasing concentrations, 0.001–10 µg of mOI-3 Alexa 488 and chOI-3 Alexa 488 to ( A ) MSTO-211H, and ( C ) NCI-H226 cells for 30 min at 4 °C. Unstained cells, mouse, and human isotype controls were used as negative controls. ( B ) and ( D ) relative median fluorescence intensity (rMFI) for all samples was calculated based on rMFI of isotype control cells for MSTO-211H and NCI-H226, respectively. A one-site binding model (Y = B max × X / (KD + X)) was used to analyze the data plotted as a hyperbolic curve (GraphPad Prism, La Jolla, USA). This analysis aimed to determine the apparent equilibrium dissociation constant (K D APP ) specific to cancer cell lines. For MSTO-211H, K D APP was 3.3 ± 0.3 nM and 9.2 ± 1.4 nM for chOI-3 and mOI-3 respectively. For NCI-H226, K D APP was 5.9 ± 1.3 nM and 11.4 ± 4.0 nM for chOI-3 and mOI-3 respectively. ( E ) MSTO-211H, and ( F ) NCI-H226 cells were stained with human unlabeled antibody ABX-MA1 and chOI-3 for 30 min at 4 °C. Following the incubation, goat anti-human Alexa 488 antibody was used for 30 min 4 °C. In all experiments, only MitoTracker Red CMXRos and DRAQ5 double positive cells were included in the analysis. Samples were collected using CytoFlex. Data are representative of at least two or three independent experiments.

Article Snippet: Anti-CD146 human mAb, ABX-MA1 was purchased from ProteoGenix (PX-TA1914, lot 110,620-A01, Schiltigheim, France).

Techniques: Flow Cytometry, Binding Assay, Fluorescence, Control, Staining, Incubation