Journal: Cells

Article Title: Muramyl Dipeptide Administration Delays Alzheimer’s Disease Physiopathology via NOD2 Receptors

doi: 10.3390/cells11142241

Figure Lengend Snippet: List of antibodies used for the Western blot and immunostaining analyses and all related information, including the name of the company, molecular weight, species, primary and secondary antibody dilution, and targets.

Article Snippet: ABCB1 , Novus biological , 170 kDa , Rabbit , NBP2-67667 , 1/1000 , IgG anti-Rabbit peroxidase , 111-035-144 , 1/2000.

Techniques: Western Blot, Immunostaining, Molecular Weight, Concentration Assay

Journal: Cells

Article Title: Muramyl Dipeptide Administration Delays Alzheimer’s Disease Physiopathology via NOD2 Receptors

doi: 10.3390/cells11142241

Figure Lengend Snippet: The long-term administration of MDP has a different effect on amyloid regarding the sex. Although, MDP induces an increase in the plaque number in the female APP Swe /PS1 treatment with MDP group. ( A ): Unbiased stereological analysis of the 6E10 + plaque number of the male and female APP Swe /PS1 treatment or not with MDP groups in the hippocampus and cortex. Analysis showed a diminution in the plaque number in the male APP Swe /PS1 treatment group in both areas and an augmentation in the Aβ plaque numbers in the female APP Swe /PS1 treatment group in the hippocampus compared to sex-matched APP Swe /PS1 controls. ( B ): Average volume per 6E10 + plaque in µm 3 of the male and female APP Swe /PS1 treatment or not groups with MDP in the hippocampus and cortex. Analysis showed no difference in both sexes and area compared to sex-matched APP Swe /PS1 controls. ( C ): The 6E10+ blood vessel frequency of male and female APP Swe /PS1 treatment or not with MDP groups. Analysis showed an augmentation in the male APP Swe /PS1 treatment group but no difference in the female APP Swe /PS1 treatment groups compared to sex-matched APP Swe /PS1 controls. ( D ): Elisa Aβ40 and Aβ42 of the male and female APP Swe /PS1 treatment or not with MDP groups. Analysis showed an increase in Aβ40 in the male APP Swe /PS1 treatment group but a decrease in the Aβ42 in APP Swe /PS1 treatment group for both sexes compared to sex-matched APP Swe /PS1 controls. ( E ): Ratio of Aβ42/Aβ40 in the male and female APP Swe /PS1 mice treatment or not with MDP groups. Analysis showed a diminution in the male APP Swe /PS1 treatment group but no difference in the female APP Swe /PS1 treatment group compared to sex-matched APP Swe /PS1 controls. ( F ); ( G ): MDP increases the clearance systems through LRP1 and ABCB1 expression in the male group. Western blot analysis of ( F ) LRP1 ( G ) ABCB1 in the male and female wild-type and APP Swe /PS1 treatment or not with MDP groups. Analysis showed an augmentation in LRP1 and ABCB1 expression in the male APP Swe /PS1 treatment group but a diminution in LRP1 and ABCB1 expression in the female APP Swe /PS1 treatment group compared to sex-matched APP Swe /PS1 controls. Data are mean ± SEM ( n = 8 animals/group) * p < 0.05, *** p < 0.001 compared to sex-matched APP Swe /PS1 controls.

Article Snippet: ABCB1 , Novus biological , 170 kDa , Rabbit , NBP2-67667 , 1/1000 , IgG anti-Rabbit peroxidase , 111-035-144 , 1/2000.

Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Western Blot

Journal: bioRxiv

Article Title: METTL16 promotes taxane resistance in Triple-Negative Breast Cancer through m 6 A-dependent translational upregulation of ABCB1

doi: 10.64898/2026.03.11.710933

Figure Lengend Snippet: A. The mRNA expression of ABCB1 was determined by qPCR in parental (sensitive) and PacR MDAMB231 and SUM159PT cells. B. ABCB1 protein levels were determined by Western analysis in these cells, with GAPDH as a loading control. C, D, E, F . ABCB1 protein levels were detected in TNBC cells (SUM159PT, MDAMB231, MDAMB468, HCC70) overexpressing METTL16 (WT ME) or in control cells with empty vector (Vec). GAPDH was used as a loading control. G, H . ABCB1 protein levels were determined in the MDAMB231 PacR and SUM159PT PacR cells overexpressing WT ME or Vec. β-actin was used as a loading control. I. ABCB1 protein levels were determined in the MDAMB231 PacR cells with METTL16 knockdown (shME-1 and shME-2). GAPDH was used as a loading control. J. Immunofluorescence staining of ABCB1 in TNBC cells. Representative images of MDAMB231 PacR cells with vector control (left) and overexpressing WT METTL16 (right) stained for ABCB1 (red) and nuclei (DAPI, blue). Scale bar: 75 µm. K, L. Paclitaxel accumulation assay using flow cytometry. Paclitaxel-Oregon green fluorescence was measured to assess intracellular drug accumulation in ( K ) MDAMB231 PacR cells expressing control shRNA (shCon) or METTL16-targeting shRNAs (shME-1 and shME-2), and ( L ) in taxane-sensitive MDAMB231 cells with empty vector (Vector) or overexpressing WT METTL16 (WTMETTL16) following treatment with Flutax-2 (500 nM) for 3 h. Increased fluorescence indicates increased intracellular drug accumulation.

Article Snippet: The antibody against METTL16 was from Cell Signaling (cat. # 87538S), and that against ABCB1 was from Proteintech Group (cat. # 22336-1-AP).

Techniques: Expressing, Western Blot, Control, Plasmid Preparation, Knockdown, Immunofluorescence, Staining, Flow Cytometry, Fluorescence, shRNA

Journal: bioRxiv

Article Title: METTL16 promotes taxane resistance in Triple-Negative Breast Cancer through m 6 A-dependent translational upregulation of ABCB1

doi: 10.64898/2026.03.11.710933

Figure Lengend Snippet: A. METTL16 RNA immunoprecipitation (RNA IP) reveals its association with ABCB1 mRNA. MDAMB231 PacR cells were transfected with FLAG-tagged WT METTL16 (Flag-ME). RNA IP was performed by using an anti-FLAG or control IgG antibody to pull down METTL16-bound RNAs, followed by qPCR analysis to detect ABCB1 or MAT2A mRNA. Upper panel: Immunoblot of the RNA IP fraction probed with anti-Flag antibody confirming efficient pull-down of Flag-METTL16. Lower panel: Enrichment of ABCB1 or MAT2A mRNA in the anti-FLAG immuno-precipitate, normalized to input. B, C, D. To determine m⁶A enrichment on ABCB1 mRNA, the Magna MeRIP m 6 A Kit (EMD Millipore) was used to perform m⁶A RNA immunoprecipitation followed by qPCR (MeRIP-qPCR) in MDAMB231 PacR cells overexpressing WT METTL16 (WT ME) or empty vector (Vector). Normal mouse IgG was used as a control. E. MeRIP-qPCR in MDAMB231 PacR cells with down-regulated METTL16 (shME) compared to non-targeting controls (shCon). Normal mouse IgG was used as a control.

Article Snippet: The antibody against METTL16 was from Cell Signaling (cat. # 87538S), and that against ABCB1 was from Proteintech Group (cat. # 22336-1-AP).

Techniques: RNA Immunoprecipitation, Transfection, Control, Western Blot, Plasmid Preparation

Journal: bioRxiv

Article Title: METTL16 promotes taxane resistance in Triple-Negative Breast Cancer through m 6 A-dependent translational upregulation of ABCB1

doi: 10.64898/2026.03.11.710933

Figure Lengend Snippet: C . Polysome profiling followed by qPCR was performed to examine the distribution of ABCB1 mRNA (left panels) and GAPDH or β-actin mRNA (right panels) across ribosomal fractions. Analyses were conducted in: ( A ) MDAMB231 PacR cells overexpressing WT METTL16 or an empty vector control; ( B ) MDAMB231 PacR cells overexpressing WT METTL16, the catalytically inactive METTL16 mutant (N184A), or an empty vector control; ( C ) taxane-sensitive MDAMB231 cells overexpressing WT METTL16, the N184A mutant, or an empty vector control. The P:M ratio was calculated by summing qPCR (ΔCt) values from polysome fractions (fractions 9-14) and normalizing to monosome fractions (fraction 6 for A , fractions 6 and 7 for B, C ).

Article Snippet: The antibody against METTL16 was from Cell Signaling (cat. # 87538S), and that against ABCB1 was from Proteintech Group (cat. # 22336-1-AP).

Techniques: Plasmid Preparation, Control, Mutagenesis