Journal: Frontiers in Endocrinology

Article Title: Transcriptome Analysis Reveal Candidate Genes and Pathways Responses to Lactate Dehydrogenase Inhibition (Oxamate) in Hyperglycemic Human Renal Proximal Epithelial Tubular Cells

doi: 10.3389/fendo.2022.785605

Figure Lengend Snippet: The protein expression levels of peroxisome proliferator-activated receptor-g coactivator 1α (PGC1α), caspase 3 (CASP3), B-cell lymphoma 2 apoptosis regulator (BCL2), BCL2 associated X apoptosis regulator (BAX), beclin1 (BECN1) and microtubule-associated proteins 1A/1B light chain 3 (MAP1LC3) in HK-2 cells treated with low D-glucose (LG) with different concentrations of oxamate (LGOXA-0mM, LGOXA-20mM, LGOXA-40mM and LGOXA-80mM) and high D-glucose (HG) with different concentrations of oxamate (HGOXA-0mM, HGOXA-20mM, HGOXA-40mM and HGOXA-80mM) for 24 hours. (A) Western blot gels. (B) Relative grey values. * indicates P -value < 0.05 after student’s t -test.

Article Snippet: The primary antibodies [Anti-PGC1α (ab54481), Anti-CASP3 (9665), Anti-BCL2 (ab59348), Anti-BAX (GB11690), Anti-BECN1 (bs-1353R), Anti-MAP1LC3 (14600-1-ap) and Anti-β-actin (GB12001)] and all secondary antibodies were purchased from abcom (Cambridge, MA, USA), Proteintech (Wuhan, China), BIOSS (Boston, Massachusetts, USA) and Servicebio (Wuhan, China), respectively.

Techniques: Expressing, Western Blot

Journal: Food Science & Nutrition

Article Title: Vitamin D ameliorates asthma‐induced lung injury by regulating HIF‐1α/Notch1 signaling during autophagy

doi: 10.1002/fsn3.2880

Figure Lengend Snippet: The primer sequences

Article Snippet: G1285 and G1243, respectively; Solarbio Life Science); interleukin (IL)‐6, IL‐10, and IL‐17 enzyme‐linked immunoassay (ELISA) kits; (Shanghai Jingtian Biotechnology Co., Ltd.); TUNEL kit (cat. no. KGA703; KeyGen Biotech); rabbit antimouse primary antibody LC 3B (cat. no. ab225383; Abcam), Beclin1 (cat. no. ab207612; Abcam), P62 (cat. no. ab91526; Abcam), HIF‐1α (cat. no. ab51608; Abcam), and Notch1 (cat. no. ab276343; Abcam); GAPDH (cat. no. ab181602; Abcam); goat antirabbit secondary antibody (batch No. SA00001‐2; Proteintech); Trizol reagent (batch No. 15596026; Ambion, Inc.); reverse transcription kit (batch no.: K1622; Thermo Scientific); real‐time fluorescent quantitative polymerase chain reaction (real‐time qPCR) kit (batch No. PA0025; Shanghai Jinan Biotechnology Co., Ltd.).

Techniques:

Journal: Food Science & Nutrition

Article Title: Vitamin D ameliorates asthma‐induced lung injury by regulating HIF‐1α/Notch1 signaling during autophagy

doi: 10.1002/fsn3.2880

Figure Lengend Snippet: Beclin1 and P62 protein expression in different groups by immunohistochemistry (IHC) (IHC, ×200). Normal: Mice treated with normal saline; Model: asthmatic model mice treated with normal saline; LD: asthmatic model mice treated with low‐dose Vit D (100 IU/kg/day); MD: asthmatic model mice treated with middle‐dose Vit D (500 IU/kg/day); HD: asthmatic model mice treated with high‐dose Vit D (1000 IU/kg/day). (a) Beclin1 protein expression in different groups. (b) P62 protein expression in different groups. *** p < .001, compared with Normal; # p < .05, ## p < .01, ### p < .001, compared with Model; $ p < .05, $$ p < .01, compared with LD group; & p < .05, compared with MD group

Article Snippet: G1285 and G1243, respectively; Solarbio Life Science); interleukin (IL)‐6, IL‐10, and IL‐17 enzyme‐linked immunoassay (ELISA) kits; (Shanghai Jingtian Biotechnology Co., Ltd.); TUNEL kit (cat. no. KGA703; KeyGen Biotech); rabbit antimouse primary antibody LC 3B (cat. no. ab225383; Abcam), Beclin1 (cat. no. ab207612; Abcam), P62 (cat. no. ab91526; Abcam), HIF‐1α (cat. no. ab51608; Abcam), and Notch1 (cat. no. ab276343; Abcam); GAPDH (cat. no. ab181602; Abcam); goat antirabbit secondary antibody (batch No. SA00001‐2; Proteintech); Trizol reagent (batch No. 15596026; Ambion, Inc.); reverse transcription kit (batch no.: K1622; Thermo Scientific); real‐time fluorescent quantitative polymerase chain reaction (real‐time qPCR) kit (batch No. PA0025; Shanghai Jinan Biotechnology Co., Ltd.).

Techniques: Expressing, Immunohistochemistry, Saline

Journal: Toxicology

Article Title: A protective role of autophagy in fine airborne particulate matter-induced apoptosis in LN-229 cells

doi: 10.1016/j.tox.2022.153271

Figure Lengend Snippet: (A-B) LN-229 cells were treated with 10, 50, and 100 μg/mL of PM2.5 for 24 h. (C-D) Cells were treated with 50 μg/mL of PM2.5 for 6, 12, 24, and 48 h. Cells without treatment were used as control. A and C are single Western blot results of expression of autophagic markers (LC3B-1, LC3B-2, and Beclin 1), while B and D are normalized band densitometry readings of Western blot results from three independent experiments (mean ± SE). (E) Immunofluorescence staining of LC3B in LN-229 cells. (F-I) LN-229 cells were pre-treated with 10 nM bafilomycin A1 (Baf A1) (F-G) or 10 nM rapamycin (Rapa) (H-I) for 4 h prior to treatment with 50 μg/mL of PM2.5 for another 24 h. F and H are single Western blot results, while G and I are normalized band densitometry readings of Western blot results from three independent experiments (mean ± SE). * Significant difference as compared with the control, p < 0.05; # Significant difference as compared with the PM2.5 group, p < 0.05.

Article Snippet: Anti-LC3B (ab51520) and anti-Beclin 1 (ab62557) antibodies were supplied by Abcam (Cambridge, UK).

Techniques: Western Blot, Expressing, Immunofluorescence, Staining

Journal: Toxicology

Article Title: A protective role of autophagy in fine airborne particulate matter-induced apoptosis in LN-229 cells

doi: 10.1016/j.tox.2022.153271

Figure Lengend Snippet: (A-B) LN-229 cells were transfected with 100 nM of Beclin 1 siRNA or control siRNA for 24 h prior to treatment with 50 μg/mL of PM2.5 for another 24 h. (C-D) LN-229 cells were pre-treated with 10 nM bafilomycin A1 (Baf A1) for 4 h prior to treatment with 50 μg/mL of PM2.5 for another 24 h. A and C are single Western blot results, while B and D are normalized band densitometry readings of Western blot results from three independent experiments (mean ± SE). (E-F) Hoechst 33342 staining was performed to identify the apoptotic cells after cells were transfected with Beclin 1 siRNA (E) or treated with Baf A1 (F) prior to 50 μg/mL of PM2.5 exposure for 24 h. * Significant difference as compared with the control that was without any treatments, p < 0.05; # Significant difference as compared with the group with only PM2.5 treatment, p < 0.05.

Article Snippet: Anti-LC3B (ab51520) and anti-Beclin 1 (ab62557) antibodies were supplied by Abcam (Cambridge, UK).

Techniques: Transfection, Western Blot, Staining

Journal: Toxicology

Article Title: A protective role of autophagy in fine airborne particulate matter-induced apoptosis in LN-229 cells

doi: 10.1016/j.tox.2022.153271

Figure Lengend Snippet: LN-229 cells were transfected with 100 nM of Beclin 1 siRNA or control siRNA for 24 h. Western blot was performed to determine the expression of Beclin 1, LC3B-1, and LC3B-2. A is a single Western blot result, while B is normalized band densitometry readings of Western blot results from three independent experiments (mean ± SE). * Significant difference as compared with the control that is without any treat-ments, p < 0.05.

Article Snippet: Anti-LC3B (ab51520) and anti-Beclin 1 (ab62557) antibodies were supplied by Abcam (Cambridge, UK).

Techniques: Transfection, Western Blot, Expressing