ab_528427 Search Results


pax 7  (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank pax 7
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pax6 antibody  (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank pax6 antibody
Pax6 Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-pax6  (Covance)
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Covance anti-pax6
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pax6 dshb cat# ab528427 antibody  (Developmental Studies Hybridoma Bank)
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Pax6 Dshb Cat# Ab528427 Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sections antibody antibody antibody mouse igm anti hnk1 rabbit anti rfp rabbit anti slug  (Developmental Studies Hybridoma Bank)
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Developmental Studies Hybridoma Bank sections antibody antibody antibody mouse igm anti hnk1 rabbit anti rfp rabbit anti slug
Sections Antibody Antibody Antibody Mouse Igm Anti Hnk1 Rabbit Anti Rfp Rabbit Anti Slug, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pax3  (Developmental Studies Hybridoma Bank)
96
Developmental Studies Hybridoma Bank pax3
a A schematic diagram illustrating our workflow utilizing CRISPR/Cas9-based prime editing of hESCs to study the effects of a specific FTO SNP on cellular differentiation. b Quantitative RT-PCR of myogenic markers in FTO rs9939609-TT and FTO rs9939609-A hESCs-derived myogenic progenitors, myocytes and myotubes. <t>Paired</t> <t>box</t> <t>3</t> ( <t>PAX3</t> ), Paired box 7 ( PAX7 ), Myogenic factor 5 ( MYF5 ), Myogenic differentiation 1 ( MYOD1 ), Myogenin ( MYOG ), Skeletal muscle actin alpha 1 ( ACTA1 ), Myosin heavy chain ( MYHC ), Neural cell adhesion molecule 1 ( NCAM1 ), Actin Beta ( ACTB ), n = 3 biologically independent samples. c Gene Set Enrichment Analysis (GSEA) of the signatures enriched in FTO rs9939609-A -myotubes, relative to FTO rs9939609-TT -myotubes, according to RNA-sequencing. d Left: western blot quantification of FTO, PAX3, PAX7, MYOD1, MYOG, MHC (myosin heavy chain, MF20) and GAPDH protein abundance in FTO rs9939609-TT -myotubes: H1, H7, H9 and FTO rs9939609-A -myotubes: FTO-H1-1, FTO-H1-2, FTO-H7-1, FTO-H7-2, FTO-H9-1, FTO-H9-2. Right: quantification of the abundance of myogenic differentiation proteins, n = 3 biologically independent samples. H1 hESC line colored in purple, H7 hESC line colored in light blue and H9 hESC line colored in dark blue. Data are presented as mean + SEM. Data were analyzed using Wald test ( c ). P values were calculated by two-tailed unpaired t-test. * P < 0.05, ** P < 0.01. Source data are provided as a Source Data file.
Pax3, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mouse ab528427 dshb β  (Developmental Studies Hybridoma Bank)
99
Developmental Studies Hybridoma Bank mouse ab528427 dshb β
a A schematic diagram illustrating our workflow utilizing CRISPR/Cas9-based prime editing of hESCs to study the effects of a specific FTO SNP on cellular differentiation. b Quantitative RT-PCR of myogenic markers in FTO rs9939609-TT and FTO rs9939609-A hESCs-derived myogenic progenitors, myocytes and myotubes. <t>Paired</t> <t>box</t> <t>3</t> ( <t>PAX3</t> ), Paired box 7 ( PAX7 ), Myogenic factor 5 ( MYF5 ), Myogenic differentiation 1 ( MYOD1 ), Myogenin ( MYOG ), Skeletal muscle actin alpha 1 ( ACTA1 ), Myosin heavy chain ( MYHC ), Neural cell adhesion molecule 1 ( NCAM1 ), Actin Beta ( ACTB ), n = 3 biologically independent samples. c Gene Set Enrichment Analysis (GSEA) of the signatures enriched in FTO rs9939609-A -myotubes, relative to FTO rs9939609-TT -myotubes, according to RNA-sequencing. d Left: western blot quantification of FTO, PAX3, PAX7, MYOD1, MYOG, MHC (myosin heavy chain, MF20) and GAPDH protein abundance in FTO rs9939609-TT -myotubes: H1, H7, H9 and FTO rs9939609-A -myotubes: FTO-H1-1, FTO-H1-2, FTO-H7-1, FTO-H7-2, FTO-H9-1, FTO-H9-2. Right: quantification of the abundance of myogenic differentiation proteins, n = 3 biologically independent samples. H1 hESC line colored in purple, H7 hESC line colored in light blue and H9 hESC line colored in dark blue. Data are presented as mean + SEM. Data were analyzed using Wald test ( c ). P values were calculated by two-tailed unpaired t-test. * P < 0.05, ** P < 0.01. Source data are provided as a Source Data file.
Mouse Ab528427 Dshb β, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ab 528427  (Developmental Studies Hybridoma Bank)
90
Developmental Studies Hybridoma Bank ab 528427
a A schematic diagram illustrating our workflow utilizing CRISPR/Cas9-based prime editing of hESCs to study the effects of a specific FTO SNP on cellular differentiation. b Quantitative RT-PCR of myogenic markers in FTO rs9939609-TT and FTO rs9939609-A hESCs-derived myogenic progenitors, myocytes and myotubes. <t>Paired</t> <t>box</t> <t>3</t> ( <t>PAX3</t> ), Paired box 7 ( PAX7 ), Myogenic factor 5 ( MYF5 ), Myogenic differentiation 1 ( MYOD1 ), Myogenin ( MYOG ), Skeletal muscle actin alpha 1 ( ACTA1 ), Myosin heavy chain ( MYHC ), Neural cell adhesion molecule 1 ( NCAM1 ), Actin Beta ( ACTB ), n = 3 biologically independent samples. c Gene Set Enrichment Analysis (GSEA) of the signatures enriched in FTO rs9939609-A -myotubes, relative to FTO rs9939609-TT -myotubes, according to RNA-sequencing. d Left: western blot quantification of FTO, PAX3, PAX7, MYOD1, MYOG, MHC (myosin heavy chain, MF20) and GAPDH protein abundance in FTO rs9939609-TT -myotubes: H1, H7, H9 and FTO rs9939609-A -myotubes: FTO-H1-1, FTO-H1-2, FTO-H7-1, FTO-H7-2, FTO-H9-1, FTO-H9-2. Right: quantification of the abundance of myogenic differentiation proteins, n = 3 biologically independent samples. H1 hESC line colored in purple, H7 hESC line colored in light blue and H9 hESC line colored in dark blue. Data are presented as mean + SEM. Data were analyzed using Wald test ( c ). P values were calculated by two-tailed unpaired t-test. * P < 0.05, ** P < 0.01. Source data are provided as a Source Data file.
Ab 528427, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ab 528427/product/Developmental Studies Hybridoma Bank
Average 90 stars, based on 1 article reviews
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ab_528427  (Developmental Studies Hybridoma Bank)
90
Developmental Studies Hybridoma Bank ab_528427
a A schematic diagram illustrating our workflow utilizing CRISPR/Cas9-based prime editing of hESCs to study the effects of a specific FTO SNP on cellular differentiation. b Quantitative RT-PCR of myogenic markers in FTO rs9939609-TT and FTO rs9939609-A hESCs-derived myogenic progenitors, myocytes and myotubes. <t>Paired</t> <t>box</t> <t>3</t> ( <t>PAX3</t> ), Paired box 7 ( PAX7 ), Myogenic factor 5 ( MYF5 ), Myogenic differentiation 1 ( MYOD1 ), Myogenin ( MYOG ), Skeletal muscle actin alpha 1 ( ACTA1 ), Myosin heavy chain ( MYHC ), Neural cell adhesion molecule 1 ( NCAM1 ), Actin Beta ( ACTB ), n = 3 biologically independent samples. c Gene Set Enrichment Analysis (GSEA) of the signatures enriched in FTO rs9939609-A -myotubes, relative to FTO rs9939609-TT -myotubes, according to RNA-sequencing. d Left: western blot quantification of FTO, PAX3, PAX7, MYOD1, MYOG, MHC (myosin heavy chain, MF20) and GAPDH protein abundance in FTO rs9939609-TT -myotubes: H1, H7, H9 and FTO rs9939609-A -myotubes: FTO-H1-1, FTO-H1-2, FTO-H7-1, FTO-H7-2, FTO-H9-1, FTO-H9-2. Right: quantification of the abundance of myogenic differentiation proteins, n = 3 biologically independent samples. H1 hESC line colored in purple, H7 hESC line colored in light blue and H9 hESC line colored in dark blue. Data are presented as mean + SEM. Data were analyzed using Wald test ( c ). P values were calculated by two-tailed unpaired t-test. * P < 0.05, ** P < 0.01. Source data are provided as a Source Data file.
Ab 528427, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ab_528427/product/Developmental Studies Hybridoma Bank
Average 90 stars, based on 1 article reviews
ab_528427 - by Bioz Stars, 2026-03
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3b5  (Developmental Studies Hybridoma Bank)
96
Developmental Studies Hybridoma Bank 3b5
a A schematic diagram illustrating our workflow utilizing CRISPR/Cas9-based prime editing of hESCs to study the effects of a specific FTO SNP on cellular differentiation. b Quantitative RT-PCR of myogenic markers in FTO rs9939609-TT and FTO rs9939609-A hESCs-derived myogenic progenitors, myocytes and myotubes. <t>Paired</t> <t>box</t> <t>3</t> ( <t>PAX3</t> ), Paired box 7 ( PAX7 ), Myogenic factor 5 ( MYF5 ), Myogenic differentiation 1 ( MYOD1 ), Myogenin ( MYOG ), Skeletal muscle actin alpha 1 ( ACTA1 ), Myosin heavy chain ( MYHC ), Neural cell adhesion molecule 1 ( NCAM1 ), Actin Beta ( ACTB ), n = 3 biologically independent samples. c Gene Set Enrichment Analysis (GSEA) of the signatures enriched in FTO rs9939609-A -myotubes, relative to FTO rs9939609-TT -myotubes, according to RNA-sequencing. d Left: western blot quantification of FTO, PAX3, PAX7, MYOD1, MYOG, MHC (myosin heavy chain, MF20) and GAPDH protein abundance in FTO rs9939609-TT -myotubes: H1, H7, H9 and FTO rs9939609-A -myotubes: FTO-H1-1, FTO-H1-2, FTO-H7-1, FTO-H7-2, FTO-H9-1, FTO-H9-2. Right: quantification of the abundance of myogenic differentiation proteins, n = 3 biologically independent samples. H1 hESC line colored in purple, H7 hESC line colored in light blue and H9 hESC line colored in dark blue. Data are presented as mean + SEM. Data were analyzed using Wald test ( c ). P values were calculated by two-tailed unpaired t-test. * P < 0.05, ** P < 0.01. Source data are provided as a Source Data file.
3b5, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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cxcr4 2b11 ebioscience  (Thermo Fisher)
86
Thermo Fisher cxcr4 2b11 ebioscience
a A schematic diagram illustrating our workflow utilizing CRISPR/Cas9-based prime editing of hESCs to study the effects of a specific FTO SNP on cellular differentiation. b Quantitative RT-PCR of myogenic markers in FTO rs9939609-TT and FTO rs9939609-A hESCs-derived myogenic progenitors, myocytes and myotubes. <t>Paired</t> <t>box</t> <t>3</t> ( <t>PAX3</t> ), Paired box 7 ( PAX7 ), Myogenic factor 5 ( MYF5 ), Myogenic differentiation 1 ( MYOD1 ), Myogenin ( MYOG ), Skeletal muscle actin alpha 1 ( ACTA1 ), Myosin heavy chain ( MYHC ), Neural cell adhesion molecule 1 ( NCAM1 ), Actin Beta ( ACTB ), n = 3 biologically independent samples. c Gene Set Enrichment Analysis (GSEA) of the signatures enriched in FTO rs9939609-A -myotubes, relative to FTO rs9939609-TT -myotubes, according to RNA-sequencing. d Left: western blot quantification of FTO, PAX3, PAX7, MYOD1, MYOG, MHC (myosin heavy chain, MF20) and GAPDH protein abundance in FTO rs9939609-TT -myotubes: H1, H7, H9 and FTO rs9939609-A -myotubes: FTO-H1-1, FTO-H1-2, FTO-H7-1, FTO-H7-2, FTO-H9-1, FTO-H9-2. Right: quantification of the abundance of myogenic differentiation proteins, n = 3 biologically independent samples. H1 hESC line colored in purple, H7 hESC line colored in light blue and H9 hESC line colored in dark blue. Data are presented as mean + SEM. Data were analyzed using Wald test ( c ). P values were calculated by two-tailed unpaired t-test. * P < 0.05, ** P < 0.01. Source data are provided as a Source Data file.
Cxcr4 2b11 Ebioscience, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibody cst 7076s myf5 santa cruz sc302 p akt cst 4051s p irs1 cst 3070s p p70s6k cst 9206s p70s6k cst 2708t pax3 dshb ab528426 pdgfrα  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology antibody cst 7076s myf5 santa cruz sc302 p akt cst 4051s p irs1 cst 3070s p p70s6k cst 9206s p70s6k cst 2708t pax3 dshb ab528426 pdgfrα
a A schematic diagram illustrating our workflow utilizing CRISPR/Cas9-based prime editing of hESCs to study the effects of a specific FTO SNP on cellular differentiation. b Quantitative RT-PCR of myogenic markers in FTO rs9939609-TT and FTO rs9939609-A hESCs-derived myogenic progenitors, myocytes and myotubes. <t>Paired</t> <t>box</t> <t>3</t> ( <t>PAX3</t> ), Paired box 7 ( PAX7 ), Myogenic factor 5 ( MYF5 ), Myogenic differentiation 1 ( MYOD1 ), Myogenin ( MYOG ), Skeletal muscle actin alpha 1 ( ACTA1 ), Myosin heavy chain ( MYHC ), Neural cell adhesion molecule 1 ( NCAM1 ), Actin Beta ( ACTB ), n = 3 biologically independent samples. c Gene Set Enrichment Analysis (GSEA) of the signatures enriched in FTO rs9939609-A -myotubes, relative to FTO rs9939609-TT -myotubes, according to RNA-sequencing. d Left: western blot quantification of FTO, PAX3, PAX7, MYOD1, MYOG, MHC (myosin heavy chain, MF20) and GAPDH protein abundance in FTO rs9939609-TT -myotubes: H1, H7, H9 and FTO rs9939609-A -myotubes: FTO-H1-1, FTO-H1-2, FTO-H7-1, FTO-H7-2, FTO-H9-1, FTO-H9-2. Right: quantification of the abundance of myogenic differentiation proteins, n = 3 biologically independent samples. H1 hESC line colored in purple, H7 hESC line colored in light blue and H9 hESC line colored in dark blue. Data are presented as mean + SEM. Data were analyzed using Wald test ( c ). P values were calculated by two-tailed unpaired t-test. * P < 0.05, ** P < 0.01. Source data are provided as a Source Data file.
Antibody Cst 7076s Myf5 Santa Cruz Sc302 P Akt Cst 4051s P Irs1 Cst 3070s P P70s6k Cst 9206s P70s6k Cst 2708t Pax3 Dshb Ab528426 Pdgfrα, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
antibody cst 7076s myf5 santa cruz sc302 p akt cst 4051s p irs1 cst 3070s p p70s6k cst 9206s p70s6k cst 2708t pax3 dshb ab528426 pdgfrα - by Bioz Stars, 2026-03
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Image Search Results


a A schematic diagram illustrating our workflow utilizing CRISPR/Cas9-based prime editing of hESCs to study the effects of a specific FTO SNP on cellular differentiation. b Quantitative RT-PCR of myogenic markers in FTO rs9939609-TT and FTO rs9939609-A hESCs-derived myogenic progenitors, myocytes and myotubes. Paired box 3 ( PAX3 ), Paired box 7 ( PAX7 ), Myogenic factor 5 ( MYF5 ), Myogenic differentiation 1 ( MYOD1 ), Myogenin ( MYOG ), Skeletal muscle actin alpha 1 ( ACTA1 ), Myosin heavy chain ( MYHC ), Neural cell adhesion molecule 1 ( NCAM1 ), Actin Beta ( ACTB ), n = 3 biologically independent samples. c Gene Set Enrichment Analysis (GSEA) of the signatures enriched in FTO rs9939609-A -myotubes, relative to FTO rs9939609-TT -myotubes, according to RNA-sequencing. d Left: western blot quantification of FTO, PAX3, PAX7, MYOD1, MYOG, MHC (myosin heavy chain, MF20) and GAPDH protein abundance in FTO rs9939609-TT -myotubes: H1, H7, H9 and FTO rs9939609-A -myotubes: FTO-H1-1, FTO-H1-2, FTO-H7-1, FTO-H7-2, FTO-H9-1, FTO-H9-2. Right: quantification of the abundance of myogenic differentiation proteins, n = 3 biologically independent samples. H1 hESC line colored in purple, H7 hESC line colored in light blue and H9 hESC line colored in dark blue. Data are presented as mean + SEM. Data were analyzed using Wald test ( c ). P values were calculated by two-tailed unpaired t-test. * P < 0.05, ** P < 0.01. Source data are provided as a Source Data file.

Journal: Nature Communications

Article Title: An obesogenic FTO allele causes accelerated development, growth and insulin resistance in human skeletal muscle cells

doi: 10.1038/s41467-024-53820-2

Figure Lengend Snippet: a A schematic diagram illustrating our workflow utilizing CRISPR/Cas9-based prime editing of hESCs to study the effects of a specific FTO SNP on cellular differentiation. b Quantitative RT-PCR of myogenic markers in FTO rs9939609-TT and FTO rs9939609-A hESCs-derived myogenic progenitors, myocytes and myotubes. Paired box 3 ( PAX3 ), Paired box 7 ( PAX7 ), Myogenic factor 5 ( MYF5 ), Myogenic differentiation 1 ( MYOD1 ), Myogenin ( MYOG ), Skeletal muscle actin alpha 1 ( ACTA1 ), Myosin heavy chain ( MYHC ), Neural cell adhesion molecule 1 ( NCAM1 ), Actin Beta ( ACTB ), n = 3 biologically independent samples. c Gene Set Enrichment Analysis (GSEA) of the signatures enriched in FTO rs9939609-A -myotubes, relative to FTO rs9939609-TT -myotubes, according to RNA-sequencing. d Left: western blot quantification of FTO, PAX3, PAX7, MYOD1, MYOG, MHC (myosin heavy chain, MF20) and GAPDH protein abundance in FTO rs9939609-TT -myotubes: H1, H7, H9 and FTO rs9939609-A -myotubes: FTO-H1-1, FTO-H1-2, FTO-H7-1, FTO-H7-2, FTO-H9-1, FTO-H9-2. Right: quantification of the abundance of myogenic differentiation proteins, n = 3 biologically independent samples. H1 hESC line colored in purple, H7 hESC line colored in light blue and H9 hESC line colored in dark blue. Data are presented as mean + SEM. Data were analyzed using Wald test ( c ). P values were calculated by two-tailed unpaired t-test. * P < 0.05, ** P < 0.01. Source data are provided as a Source Data file.

Article Snippet: Blots were incubated with primary antibodies: FTO (ab94482; 1:1000; Abcam), PAX3 (AB528426; 0.5 μg/ml; DSHB), PAX7 (AB528428; 0.5 μg/ml; DSHB), MYOD1 (sc-377460; 1:200; Santa Cruz), MYOG (sc-52903; 1:200; Santa Cruz), MHC/MF20 (AB2147781; 0.5 μg/ml; DSHB), p-IRS1 (2385S; 1:1000; Cell Signaling), p-FOXO1 (9461S; 1:1000; Cell Signaling), p-AKT (4060S; 1:1000; Cell Signaling), IGF2 (ab9574; 1:700; Abcam) and GAPDH (2118S; 1:1000; Cell Signaling), then probed with the secondary antibody anti-mouse IgG HRP (7076S; 1:1000; Cell Signaling) or anti-rabbit IgG HRP (7074S; 1:1000; Cell Signaling).

Techniques: CRISPR, Cell Differentiation, Quantitative RT-PCR, Derivative Assay, RNA Sequencing, Western Blot, Quantitative Proteomics, Two Tailed Test