ab_2621049 Search Results


ab_2621049  (Bethyl)
90
Bethyl ab_2621049
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Ab 2621049, supplied by Bethyl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ab_2621049 - by Bioz Stars, 2026-03
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Journal: Molecular cell

Article Title: Crosstalk between RNA Pol II C-Terminal Domain Acetylation and Phosphorylation via RPRD Proteins

doi: 10.1016/j.molcel.2019.04.008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies RPRD1B (Rabbit) Bethyl AB_11218400 RPRD1B (Mouse) Novus AB_2285416 RRPD1A Santa Cruz sc514724 Pol II (8WG16) Abcam AB_306327 Pol II (4H8) Abcam AB_304868 Pol II (H224) Santa Cruz AB_2268548 Pol II (K7ac) This Paper N/A RPAP3 Bethyl AB_2621049 HA (Rat) Sigma AB_10094468 HA Agarose Bead Sigma AB_257974 HA Magnetic Bead Sigma AB_2749815 Normal IgG (Rabbit) Santa Cruz AB_737197 Normal IgG (Mouse) Santa Cruz AB_737182 HSP90 Abcam AB_444729 HDAC1 Abcam AB_2264059 AffiniPure Goat Anti-Mouse HRP Jackson Immunoresearch AB_10015289 AffiniPure Goat Anti-Rabbit HRP Jackson Immunoresearch AB_2313567 AffiniPure Goat Anti-Rat HRP Jackson Immunoresearch AB_2338128 Chemicals, Peptides, and Recombinant Proteins Panobinostat Santa Cruz CAS 404950-80-7 Nicotinamide Sigma CAS 98-92-0 α-Amanitin Santa Cruz CAS 23109-05-9 CTD Peptides Peptide 2.0 N/A Critical Commercial Assays QIAquick PCR Purification Kit Qiagen 28104 RNeasy Plus Mini Kit Qiagen 74136 Ovation Ultralow System V2 NuGEN 0344-32 Deposited Data RNA Pol II ChIP-seq Data GEO GSE124996 RNA Seq Data GEO GSE125486 Western Blotting Data Mendeley Data DOI: 10.17632/f6w4wzb9dr.1 Structural Modeling Mendeley Data DOI: 10.17632/f6w4wzb9dr.1 SILAC Data This Paper Table S1 Experimental Models: Cell Lines NIH3T3 ATCC CRL-1658 293T ATCC CRL-3216 Software and Algorithms Skewer v 0.1.124 Jiang et al., 2014 N/A Bowtie v 1.1.2 Langmead et al., 2009 N/A MACS2 v 2.1.0.20150731 Zhang et al., 2008 N/A DeepTools v 2.2.3 Ramirez et al., 2016 N/A MaxQuant v 1.2.5.8 Cox and Mann, 2008 N/A Open in a separate window KEY RESOURCES TABLE RPRD proteins interact with the RNA Pol II CTD in an acetylation-dependent manner RPRD CTD-interacting domain amide residues stabilize acetylated CTD heptads KDAC inhibition enahnces Pol II CTD K7-acetylation & reduces S5-phosphorylation RPRD1B knockdown disrupts Pol II CTD deacetylase & CTD phosphatase activity

Techniques: Recombinant, Purification, RNA Sequencing Assay, Western Blot, Software