aav6 Search Results


raav6 virus  (PackGene Biotech lnc)
95
PackGene Biotech lnc raav6 virus
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Raav6 Virus, supplied by PackGene Biotech lnc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/AAV6+Vector+Production/pmc10850376-39-1-6
Average 95 stars, based on 1 article reviews
raav6 virus - by Bioz Stars, 2026-10
95/100 stars
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aav6  (OriGene)
90
OriGene aav6
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/Adeno-Associated+Virus+6+%2F+AAV6+(intact+particle)+Mouse+Monoclonal+Antibody/pmc04729438-162-4-2
Average 90 stars, based on 1 article reviews
aav6 - by Bioz Stars, 2026-10
90/100 stars
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aav6 gfp  (Vector Biolabs)
95
Vector Biolabs aav6 gfp
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6 Gfp, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/AAV6-GFP/us11920158-335-3-4
Average 95 stars, based on 1 article reviews
aav6 gfp - by Bioz Stars, 2026-10
95/100 stars
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control aav6  (Vector Biolabs)
95
Vector Biolabs control aav6
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Control Aav6, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/AAV6-GFP-U6-shRNA/pmc09226085-74-9-13
Average 95 stars, based on 1 article reviews
control aav6 - by Bioz Stars, 2026-10
95/100 stars
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aav6 cag cre gfp  (Vector Biolabs)
95
Vector Biolabs aav6 cag cre gfp
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6 Cag Cre Gfp, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/AAV6-CAG-GFP/pm34453065-127-5-6
Average 95 stars, based on 1 article reviews
aav6 cag cre gfp - by Bioz Stars, 2026-10
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aav6 2 camkiiα ert2 cre  (Vector Biolabs)
95
Vector Biolabs aav6 2 camkiiα ert2 cre
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6 2 Camkiiα Ert2 Cre, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/AAV6-Cre/pmc11193045-16-0-2
Average 95 stars, based on 1 article reviews
aav6 2 camkiiα ert2 cre - by Bioz Stars, 2026-10
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aav cre gfp  (Vector Biolabs)
90
Vector Biolabs aav cre gfp
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav Cre Gfp, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/AAV6-Cre-GFP/pmc08895126-295-0-9
Average 90 stars, based on 1 article reviews
aav cre gfp - by Bioz Stars, 2026-10
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aav6 cmv con  (Vector Biolabs)
92
Vector Biolabs aav6 cmv con
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6 Cmv Con, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/AAV6+Null/pm37371128-69-30-33
Average 92 stars, based on 1 article reviews
aav6 cmv con - by Bioz Stars, 2026-10
92/100 stars
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recombinant aav6 plasmids  (GenScript corporation)
90
GenScript corporation recombinant aav6 plasmids
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Recombinant Aav6 Plasmids, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/recombinant+aav6+plasmids/pmc11559603-279-41-44
Average 90 stars, based on 1 article reviews
recombinant aav6 plasmids - by Bioz Stars, 2026-10
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cmv-gfp (2.89 × 10 9 tu)  (SignaGen)
90
SignaGen cmv-gfp (2.89 × 10 9 tu)
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Cmv Gfp (2.89 × 10 9 Tu), supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/aav6+cmv+gfp/pmc07160358-162-21-27
Average 90 stars, based on 1 article reviews
cmv-gfp (2.89 × 10 9 tu) - by Bioz Stars, 2026-10
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aav6-shklf6  (Shanghai GenePharma)
90
Shanghai GenePharma aav6-shklf6
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6 Shklf6, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/aav6+recombinant+vector/pm40234972-173-0-6
Average 90 stars, based on 1 article reviews
aav6-shklf6 - by Bioz Stars, 2026-10
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aav6.2ff-adi-15878  (Marburg GmbH)
90
Marburg GmbH aav6.2ff-adi-15878
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6.2ff Adi 15878, supplied by Marburg GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/aav6+2ff+adi+15878/pm36092367-93-36-67
Average 90 stars, based on 1 article reviews
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Image Search Results


Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the rAAV6 homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.

Journal: Frontiers in Cell and Developmental Biology

Article Title: In situ correction of various β-thalassemia mutations in human hematopoietic stem cells

doi: 10.3389/fcell.2023.1276890

Figure Lengend Snippet: Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the rAAV6 homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.

Article Snippet: The rAAV6 virus was purchased from PackGene, and the titer value was determined to be 1E+13 GC/mL by quantitative PCR detection.

Techniques: Derivative Assay, Selection, Marker, Flow Cytometry, Expressing