aav6 Search Results


raav6 virus  (PackGene Biotech lnc)
95
PackGene Biotech lnc raav6 virus
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Raav6 Virus, supplied by PackGene Biotech lnc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/pmc10850376-39-1-6?v=PackGene+Biotech+lnc
Average 95 stars, based on 1 article reviews
raav6 virus - by Bioz Stars, 2026-07
95/100 stars
  Buy from Supplier
aav cre gfp  (Vector Biolabs)
90
Vector Biolabs aav cre gfp
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav Cre Gfp, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/pmc08895126-295-0-9?v=Vector+Biolabs
Average 90 stars, based on 1 article reviews
aav cre gfp - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
aav6  (OriGene)
90
OriGene aav6
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/pmc04729438-162-4-2?v=OriGene
Average 90 stars, based on 1 article reviews
aav6 - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
control aav6  (Vector Biolabs)
95
Vector Biolabs control aav6
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Control Aav6, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/pmc09510876-265-10-16?v=Vector+Biolabs
Average 95 stars, based on 1 article reviews
control aav6 - by Bioz Stars, 2026-07
95/100 stars
  Buy from Supplier
aav6 gfp  (Vector Biolabs)
95
Vector Biolabs aav6 gfp
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6 Gfp, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/us11920158-335-3-4?v=Vector+Biolabs
Average 95 stars, based on 1 article reviews
aav6 gfp - by Bioz Stars, 2026-07
95/100 stars
  Buy from Supplier
aav6 cag cre gfp  (Vector Biolabs)
95
Vector Biolabs aav6 cag cre gfp
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6 Cag Cre Gfp, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/pm34453065-127-5-6?v=Vector+Biolabs
Average 95 stars, based on 1 article reviews
aav6 cag cre gfp - by Bioz Stars, 2026-07
95/100 stars
  Buy from Supplier
aav6 2 camkiiα ert2 cre  (Vector Biolabs)
95
Vector Biolabs aav6 2 camkiiα ert2 cre
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6 2 Camkiiα Ert2 Cre, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/pmc11193045-16-0-2?v=Vector+Biolabs
Average 95 stars, based on 1 article reviews
aav6 2 camkiiα ert2 cre - by Bioz Stars, 2026-07
95/100 stars
  Buy from Supplier
aav6 cmv con  (Vector Biolabs)
92
Vector Biolabs aav6 cmv con
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6 Cmv Con, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/pm37371128-69-30-33?v=Vector+Biolabs
Average 92 stars, based on 1 article reviews
aav6 cmv con - by Bioz Stars, 2026-07
92/100 stars
  Buy from Supplier
aav8-u6-mir29b-cmv-gfp  (SignaGen)
90
SignaGen aav8-u6-mir29b-cmv-gfp
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav8 U6 Mir29b Cmv Gfp, supplied by SignaGen, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/pmc06784548-12-0-2?v=SignaGen
Average 90 stars, based on 1 article reviews
aav8-u6-mir29b-cmv-gfp - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
recombinant aav6 plasmids  (GenScript corporation)
90
GenScript corporation recombinant aav6 plasmids
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Recombinant Aav6 Plasmids, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/pmc11559603-279-41-44?v=GenScript+corporation
Average 90 stars, based on 1 article reviews
recombinant aav6 plasmids - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
aav6.2ff capsid  (BioTherapeutics Inc)
90
BioTherapeutics Inc aav6.2ff capsid
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6.2ff Capsid, supplied by BioTherapeutics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/pmc10701082-278-16-32?v=BioTherapeutics+Inc
Average 90 stars, based on 1 article reviews
aav6.2ff capsid - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier
aav6-ef1a-b19g  (Applied Viromics LLC)
90
Applied Viromics LLC aav6-ef1a-b19g
Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the <t>rAAV6</t> homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.
Aav6 Ef1a B19g, supplied by Applied Viromics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/aav6/pmc09844990-86-7-11?v=Applied+Viromics+LLC
Average 90 stars, based on 1 article reviews
aav6-ef1a-b19g - by Bioz Stars, 2026-07
90/100 stars
  Buy from Supplier

Image Search Results


Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the rAAV6 homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.

Journal: Frontiers in Cell and Developmental Biology

Article Title: In situ correction of various β-thalassemia mutations in human hematopoietic stem cells

doi: 10.3389/fcell.2023.1276890

Figure Lengend Snippet: Development of a universal HBB gene correction approach with high HDR efficiency by enrichment of HBB-targeted HSCs. All experiments in this figure were performed on cord blood-derived CD34 + cells. (A) Schematic overview of the gene targeting strategy for human HBB locus following DSB initiated by Cas9/sgRNA and HDR using the rAAV6 homologous donor as a repair template. Dark blue boxes: HBB exons; light blue boxes: HBB introns; purple boxes: homology arms; green boxes: EGFP selection marker. In–out PCR analysis of the positive colonies with homology-directed repair (HDR) using three primers. (B) Pie chart statistics of the colonies’ genotype. A total of 214 colonies were verified. PCR analysis was applied to identify the genotype of methylcellulose colonies from EGFP + HSCs. (C) HDR efficiency and viability were analyzed via flow cytometry after HSCs were treated with polybrene and small-molecule compounds such as SCR7 and L755507. (D) Representative images of the differentiated HSCs on day 21 showing lineage-restricted progenitors (CFU-GM, BFU-E, and CFU-E) and multipotent progenitors (CFU-GEMM) with EGFP expression. The ratio of different types of colonies was counted. Values are presented as the mean ± SD for quadruplicate samples from a representative experiment. p -values were calculated using one-way ANOVA. ** indicates <0.01.

Article Snippet: The rAAV6 virus was purchased from PackGene, and the titer value was determined to be 1E+13 GC/mL by quantitative PCR detection.

Techniques: Derivative Assay, Selection, Marker, Flow Cytometry, Expressing