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Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
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Vector Biolabs aav1 mcherry
Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
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Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
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Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
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Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
Virus Strains Aav1 2 Cag Egfp Vector Biolab N A Aav1, supplied by Vector Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Vector Biolabs aav1 2 cag icre egfp
Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
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Vector Biolabs aav1 lacz
Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
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SignaGen aav2/5-hsyn-mcherry
Nrxn3 <t>shRNA</t> reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control <t>(scrambled</t> <t>sequence)</t> shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.
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Image Search Results


Nrxn3 shRNA reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control (scrambled sequence) shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.

Journal: Neurobiology of Pain

Article Title: Nrxn3 reduces myofascial nociceptive pain

doi: 10.1016/j.ynpai.2025.100197

Figure Lengend Snippet: Nrxn3 shRNA reduced Nrxn3 expression The central amygdala was infused with adeno-associated virus containing either a control (scrambled sequence) shRNA or a Nrxn3 shRNA expression construct. After four weeks the masseter tendon was ligated bilaterally, or a sham surgery was performed and the animals sacrificed three weeks after ligature. Central amygdala tissue was isolated by biopsy punches of frozen sections and the quantity of Nrxn3 was quantitated by ELISA. Significant differences of (P < 0.05) between the groups are indicated by “*”. Each point on the graph is from a different animal. Values are the means ± SEM for 8 animals per treatment group.

Article Snippet: A separate group of rats was infused with 1 μl of 1X10 13 TU/mL AAV1 containing a scrambled shRNA sequence (AAV1-GFP-U6-shRNA, Vector Biolabs).

Techniques: shRNA, Expressing, Virus, Control, Sequencing, Construct, Isolation, Enzyme-linked Immunosorbent Assay