Journal: The Journal of Neuroscience

Article Title: Synaptic Specificity and Application of Anterograde Transsynaptic AAV for Probing Neural Circuitry

doi: 10.1523/JNEUROSCI.2158-19.2020

Figure Lengend Snippet: List of viruses used in this study

Article Snippet: In addition, we observed that AAV1-hSyn-Flp was capable of labeling comparable numbers of cells in SC compared with Cre-expressing AAV1 constructs ( C , D ), suggesting this approach may be applied interchangeably with AAV1 viruses expressing different forms of recombinase. table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Virus Titer Source Addgene Plasmid ID AAV1 hSyn-Cre-WPRE 2.5 × 10 13 GC/ml Addgene 105553 scAAV1 hSyn-Cre 2.8 × 10 13 GC/ml Vigene Biosciences AAV1 hSyn-Flp 5.5 × 10 13 GC/ml Vigene Biosciences 51669 AAVretro-hSyn-Cre 1.5 × 10 14 GC/ml Vigene Biosciences 105537 AAV1-CMV-Cre 2.7 × 10 13 GC/ml Addgene 105537 AAV5-CMV-Cre 2.8 × 10 13 GC/ml Addgene 105537 AAV6-CMV-Cre 3.5 × 10 13 GC/ml Addgene 105537 AAV8-CMV-Cre 4.4 × 10 13 GC/ml Addgene 105537 AAV9-CMV-Cre 1.6 × 10 14 GC/ml Addgene 105537 AAVPHP.B-CMV-Cre 2.3 × 10 13 GC/ml SignaGen CAV2-CMV-Cre 1.3 × 10 12 GC/ml Montpellier Viral Core Adenovirus (Ad5-CMV-Cre) 3.0 × 10 12 GC/ml Kerafast Lentivirus (LV-CMV-Cre) 1.0 × 10 8 GC/ml Cellomics Tech Baculovirus (BAC-CMV-Cre) 3.7 × 10 10 GC/ml Uni. of Iowa G-deleted Rabies virus (RV-Cre-GFP) 8.6 × 10 8 GC/ml Salk Institute G-deleted Rabies virus (RV-GFP) 5.5 × 10 8 GC/ml Salk Institute AAV1-EF1a-DIO-Flp-WPRE 1.5 × 10 14 GC/ml Vigene Biosciences 87306 AAV d J-EF1a-fDIO-YFP-WPRE 2.5 × 10 13 GC/ml UNC Viral Core 55641 AAV1-CAG-FLEX-GFP-WPRE 1.7 × 10 13 GC/ml Addgene 51502 AAVretro-hSyn-GFP-WPRE 1.7 × 10 14 GC/ml Vigene Biosciences 105539 AAV1 hSyn-GFP-WPRE 3.2 × 10 13 GC/ml Addgene 105539 AAV1-EF1a-DIO-hChR2-eYFP-WPRE 1.6 × 10 13 GC/ml Addgene 20298 AAV d J-CMV-TeNT-P2A-GFP 5.7 × 10 12 GC/ml Stanford Viral Core AAVretro-EF1a-Cre-WPRE 2.3 × 10 13 GC/ml Salk Institute 55636 Open in a separate window List of viruses used in this study Moreover, among the four AAV1 viruses tested, we observed a nearly twofold increase in the number of anterograde transsynaptically labeled cells when using AAV1-hSyn-Cre-WPRE compared with scAAV1-hSyn-Cre, AAV1-CMV-Cre, or AAV1-hSyn-Flp, which each lack the WPRE ( D ; ).

Techniques: Plasmid Preparation