Journal: Discover Oncology

Article Title: Penicilazaphilone C triggers ferroptosis in triple-negative breast cancer cells via the MDM2/p53/SLC7 A11/GPX4 pathway

doi: 10.1007/s12672-025-02687-w

Figure Lengend Snippet: PAC triggers various forms of cell death in TNBC cells. A–C JC-1 staining and FCM analysis were conducted on MDA-MB-231 and MDA-MB-436 cells to determine the percentage of JC-1 monomers ( B ) and aggregates ( C ). Flow cytometric analysis of 7-AAD-positive MDA-MB-231 and MDA-MB-436 cells was performed, with representative images shown ( D ) and quantified analysis of five samples ( E ). F A lactate dehydrogenase (LDH) release assay was utilized to quantify LDH levels in the supernatant of MDA-MB-231 and MDA-MB-436 cells. The data, presented as the mean ± standard deviation of five and ten ( F ) independent replicates, were statistically analyzed using one-way ( C ) or two-way ANOVA, followed by post hoc multiple comparisons. Nonsignificant differences are denoted by identical letters above the columns, while significant differences (p < 0.05) are indicated by distinct letters

Article Snippet: 7-AAD (HY-D1020) was acquired from MedChemExpress (Shanghai, China) and dissolved in phosphate-buffered saline (PBS) as per the manufacturer's instructions.

Techniques: Staining, Lactate Dehydrogenase Assay, Standard Deviation

Journal: Discover Oncology

Article Title: Penicilazaphilone C triggers ferroptosis in triple-negative breast cancer cells via the MDM2/p53/SLC7 A11/GPX4 pathway

doi: 10.1007/s12672-025-02687-w

Figure Lengend Snippet: PAC inhibits TNBC cell death mainly by inducing ferroptosis. A Flow cytometry analysis of the percentage of 7-AAD-positive MDA-MB-231 and MDA-MB-436 cells after treatment with PAC alone and in combination with three inhibitors, as indicated. B Flow cytometry analysis of lipid peroxidation using BODIPY-C11 was performed on MDA-MB-231 and MDA-MB-436 cells treated as described in A . C Flow cytometry assessment of intracellular Fe 2+ levels in MDA-MB-231 and MDA-MB-436 cells treated as described in A . The data are presented as the mean ± standard deviation of five independent replicates and are analyzed using a two-way ANOVA, followed by post hoc multiple comparisons. Identical letters above columns denote nonsignificant differences, whereas distinct letters indicate significant differences with a p-value of less than 0.05

Article Snippet: 7-AAD (HY-D1020) was acquired from MedChemExpress (Shanghai, China) and dissolved in phosphate-buffered saline (PBS) as per the manufacturer's instructions.

Techniques: Flow Cytometry, Standard Deviation