Journal: Pharmaceuticals

Article Title: Self-Assembled Supramolecular Nanoparticles Improve the Cytotoxic Efficacy of CK2 Inhibitor THN7

doi: 10.3390/ph11010010

Figure Lengend Snippet: Cytotoxic activity of THN7-loaded α-cyclodextrin nanoparticles on A-427 lung cancer cells. C4: C 4 H 9 ; C6: C 6 H 13 ; C8: C 8 H 17 . (+) loaded cyclodextrins; (−) empty cyclodextrins. Viability of A427 cells were determined using an MTT assay. The viability of cells treated with 1% of the appropriate solvent was set 100%. Staurosporine served as a control for the correctly performance of the MTT assay using A427 cells. In all experiments THN7 was used at a final concentration of 6 μM.

Article Snippet: The nanoparticles were tested on the human lung cancer cell line A427, which was obtained from the German Collection of Microorganisms and Cell Culture (DSMZ, Braunschweig, Germany).

Techniques: Activity Assay, MTT Assay, Solvent, Control, Concentration Assay

Journal: Oncotarget

Article Title: Growth-stimulatory activity of TIMP-2 is mediated through c-Src activation followed by activation of FAK, PI3-kinase/AKT, and ERK1/2 independent of MMP inhibition in lung adenocarcinoma cells

doi:

Figure Lengend Snippet: We used A549 A. NCI-H2009 B. SK-LU-1 C. HCC-827 D. and A427 E. cells to perform BrdU incorporation assays. Lung adenocarcimoma cell lines were serum-starved in the presence of various concentrations of TIMP-2 or TIMP-2 C72S for 48 hr and then BrdU incorporation was evaluated. Standard deviations were calculated from experiments performed in triplicate in three independent assays. Statistical significance is indicated. * p < 0.05 ** p < 0.01 *** p < 0.001 when compared with untreated cells.

Article Snippet: A549 cells were obtained from the American Type Culture Collection and SK-LU-1, HCC-827, and A427 cells were purchased from the Korean Cell Line Bank.

Techniques: BrdU Incorporation Assay