Journal:

Article Title: Adaptation of Very Virulent Infectious Bursal Disease Virus to Chicken Embryonic Fibroblasts by Site-Directed Mutagenesis of Residues 279 and 284 of Viral Coat Protein VP2

doi:

Figure Lengend Snippet: Reactivities of various MAbs to different IBDV strains

Article Snippet: Five monoclonal antibodies (MAbs) (3-1, 9-6, 17-82, 39A, and 44-18) raised against virulent strain 002/73 of IBDV by Fahey et al. ( 4 ) and three MAbs (B29, R63, and R69) raised against vaccine strain D78 by Snyder et al. ( 11 ) were used in antigen capture enzyme-linked immunosorbent assays (ELISA) to examine their immunoreactivities to several CEF-nonpermissive IBDV strains isolated from Hong Kong and China ( 3 ) and to two CEF-adapted strains, including the NT mutant derived from HK46 (HK46-NT) and strain D78 (ATCC VR-2041).

Techniques: Mutagenesis

Journal:

Article Title: Adaptation of Very Virulent Infectious Bursal Disease Virus to Chicken Embryonic Fibroblasts by Site-Directed Mutagenesis of Residues 279 and 284 of Viral Coat Protein VP2

doi:

Figure Lengend Snippet: Cytopathogenicity of recombinant virus on CEF cells. CEF cells (2 × 104 in each well) were mixed with 100 TCID50 of D78 or HK46-NT. At daily intervals (up to 5 days), 20 μl of MTS/PES solution was added to each well and the plates were incubated at 37°C for 2 h in a humidified chamber with 5% CO2. The plates were then measured at OD490. The background reading, measured from wells with culture medium and MTS/PES only, was subtracted from the data. Each value is the average of two independent experiments.

Article Snippet: Five monoclonal antibodies (MAbs) (3-1, 9-6, 17-82, 39A, and 44-18) raised against virulent strain 002/73 of IBDV by Fahey et al. ( 4 ) and three MAbs (B29, R63, and R69) raised against vaccine strain D78 by Snyder et al. ( 11 ) were used in antigen capture enzyme-linked immunosorbent assays (ELISA) to examine their immunoreactivities to several CEF-nonpermissive IBDV strains isolated from Hong Kong and China ( 3 ) and to two CEF-adapted strains, including the NT mutant derived from HK46 (HK46-NT) and strain D78 (ATCC VR-2041).

Techniques: Recombinant, Incubation

Journal:

Article Title: Adaptation of Very Virulent Infectious Bursal Disease Virus to Chicken Embryonic Fibroblasts by Site-Directed Mutagenesis of Residues 279 and 284 of Viral Coat Protein VP2

doi:

Figure Lengend Snippet: Growth curve of the mutant virus. CEF cells were infected with 100 TCID50 of D78 or HK46-NT in 200 μl of culture medium. Virus harvested at daily intervals was then titered and expressed as TCID50 per milliliter. Each value is the average of two independent experiments.

Article Snippet: Five monoclonal antibodies (MAbs) (3-1, 9-6, 17-82, 39A, and 44-18) raised against virulent strain 002/73 of IBDV by Fahey et al. ( 4 ) and three MAbs (B29, R63, and R69) raised against vaccine strain D78 by Snyder et al. ( 11 ) were used in antigen capture enzyme-linked immunosorbent assays (ELISA) to examine their immunoreactivities to several CEF-nonpermissive IBDV strains isolated from Hong Kong and China ( 3 ) and to two CEF-adapted strains, including the NT mutant derived from HK46 (HK46-NT) and strain D78 (ATCC VR-2041).

Techniques: Mutagenesis, Infection

Journal: Differentiation; research in biological diversity

Article Title: Differentiation and sarcomere formation in skeletal myocytes directly prepared from human induced pluripotent stem cells using a sphere-based culture

doi: 10.1016/j.diff.2017.07.004

Figure Lengend Snippet: Primary antibodies used for muscle cell identification.

Article Snippet: We repeated three independent experiments for cell counting. table ft1 table-wrap mode="anchored" t5 caption a7 Antibody Clone Company Dilution Pax7 Pax7 DSHB (Iowa City, IA, USA) 1:40 Pax3 Pax3 DSHB (Iowa City, IA, USA) 1:40 MyoD 5.8A Vector laboratories (Burlingame, CA, USA) 1:40 MyoG (mouse monoclonal) F5D DSHB 1:40 MyoG (rabbit polyclonal) M-225 Santa Cruz (Dallas, TX, USA) 1:400 MHC MF20 DSHB 1:40 MHC (Alexa Fluor 480 or Alexa Fluor 660 conjugated) MF20 eBioscience (San Diego, CA, USA) 1:500 MYH3 (embryonic MHC) F1.652 DSHB 1:40 MYH8 (fetal/perinatal MHC) N3.36 DSHB 1:40 MYH2 (adult MHC) A4.74 DSHB 1:40 Titin 9D10 DSHB 1:40 Laminin L9393 Sigma-Aldrich 1:100 Open in a separate window MyoG, Myogenin; DSHB, Developmental Study Hybridoma Bank.

Techniques: Plasmid Preparation

Journal: Acta Veterinaria Scandinavica

Article Title: The influence of the PRKAG3 mutation on glycogen, enzyme activities and fibre types in different skeletal muscles of exercise trained pigs

doi: 10.1186/1751-0147-53-20

Figure Lengend Snippet: Photomicrographs of serial sections of m. longissimus dorsi of carriers (A, B, C) and non-carriers (D, E, F) of the PRKAG3 mutation . Fibre types I, IIA, and IIB classified with myosin ATPase (pH 4.6) stains (A, D) and fibre type IIAX is classified with immunohistochemical (A4-74) stains (B, E). Note that many type IIB fibres in the myosin ATPase stain were classified as IIAX fibres with the immunohistochemical stain and that some of these IIAX fibres may be pure, IIX or IIBX fibres. Oxidative capacity is evaluated from the NADH tetrazolium reductase stains (C, F). Note that type I fibres have a high staining intensity, whereas staining intensity varies among the subgroups of type II fibres.

Article Snippet: Serial sections, were reacted with myosin heavy chain (MHC) antibodies BA-D5 (MHCI) (gift from E.Barrey) and A4-74 (MHCIIA + MHCIIX) (Alexis Biochemicals).

Techniques: Mutagenesis, Immunohistochemical staining, Staining