Journal: International journal of cancer

Article Title: Human malignant glioma cell lines are sensitive to low radiation doses.

doi: 10.1002/ijc.11033

Figure Lengend Snippet: FIGURE 1 – Survival of human glioma cells following irradiation. Cells were irradiated with 0–2 Gy. For all experiments, all data are in sextuplicates. Errors bars represent SD. (a) G111, G142 and G152 glioma cell lines display HRS at doses below 1 Gy (p 0.05 for 0.7–0.9 or 0.8–1.0 Gy). (b) HRS was observed only with G5 cells (p 0.05 for 0.8–1.0 Gy), whereas its clone, CL35, displayed conventional sensitivity to radiation therapy. (c) HRS was observed with the radioresistant melanoma cell lines M4Be, A375P, MeWo and SKMel2 (p 0.05 for 0.7–0.9 Gy). (d) MRC5 human fibroblasts exhibit HRS (p 0.05 for 0.7–0.9 Gy). The radiation responses of the radiosensitive tumor cell lines H460 (lung cancer) and MCF7 (breast cancer) do not exhibit HRS but rather the LQ model.

Article Snippet: Four human melanoma cell lines were used: SKMel2, MeWo and A375P were obtained from the ATCC (Bethesda, MD) and M4Be was established in our laboratory.13 Cells were grown in McCoy’s 5A medium with L-glutamine supplemented with 10% FBS, which was heat-inactivated at 56°C for 30 min, 100 units/ml penicillin and 100 mg/ml streptomycin.

Techniques: Irradiation

Journal: iScience

Article Title: Heterotypic cell-in-cell structures between cancer and NK cells are associated with enhanced anticancer drug resistance

doi: 10.1016/j.isci.2022.105017

Figure Lengend Snippet: Analysis of heterotypic CIC structures between hABCB1 overexpressing cancer and NK-92 cells (A and B) Cell proliferation of wild-type or hABCB1 overexpressing A549 or A375SM cells was measured by CCK-8 assay after treatment with various concentrations of doxorubicin and paclitaxel (0.5, 1, 2, 5 and 10 μM) for 24 h. p values were determined using the student’s t- tests. All experiments were performed in triplicate. ∗, p < 0.05; ∗∗, p < 0.01. (C) A549 and A375SM wild-type or hABCB1 overexpressing cells were co-cultured with NK-92 cells for 4 h to analyze the heterotypic CIC population. (D) Heterotypic CIC populations in NK-92 injected A549 and A375SM wild-type or hABCB1 overexpressing tumors of xenograft animal models were analyzed using flow cytometry. ∗, p < 0.05; ns, not significant. (E) Cell proliferation of wild-type or hABCB1 overexpressing cancer organoids (SNU-CO-1) were measured by CCK-8 assay after treatment with various concentrations of doxorubicin and paclitaxel (0.5, 1 and 5 μM) for 24 h. (F) The wild-type or hABCB1 overexpressing cancer organoids (SNU-CO-1) were co-cultured with NK-92 cells for 4 h to analyze the heterotypic CIC population. (G) Heterotypic CIC and non-CIC formed by co-culture of A549 and NK-92 cells were isolated, and the differences in NK susceptibility and drug resistance were analyzed. The mechanistic model of this study is that the heterotypic CIC populations exhibit higher activation of signaling proteins such as Akt, IkBα, Shc, Raf and Cyclin D1, increased cancer cell growth and drug resistance, and decreased NK sensitivity compared to non-CIC populations.

Article Snippet: The A375SM (human melanoma) cell line and cancer organoids (SNU-1-CO) are obtained from Korea Cell Line Bank (KCLB, Seoul, Republic of Korea).

Techniques: CCK-8 Assay, Cell Culture, Injection, Flow Cytometry, Co-Culture Assay, Isolation, Activation Assay

Journal: iScience

Article Title: Heterotypic cell-in-cell structures between cancer and NK cells are associated with enhanced anticancer drug resistance

doi: 10.1016/j.isci.2022.105017

Figure Lengend Snippet:

Article Snippet: The A375SM (human melanoma) cell line and cancer organoids (SNU-1-CO) are obtained from Korea Cell Line Bank (KCLB, Seoul, Republic of Korea).

Techniques: Virus, Recombinant, Staining, CCK-8 Assay, Ab Array, Software