a263insgg Search Results


a263insgg  (Addgene inc)
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Addgene inc a263insgg
Representative larvae of a crossing between heterozygous HSPCre13 males and heterozygous A263ins6 females. The F1 offspring were heat-shocked for two hours at 34°C at early gastrula (stage 11). A: Comparison of a larva overexpressing the <t>A263insGG</t> HNF1β mutation with a non-transgenic control. Abbreviations: a: thickened anus; g: abnormal gut; e: reduced eye; t: abnormal tail; bracket: edema. Red fluorescence: HSPCre13 transgene. Blue fluorescence: 328del4 transgene. HS: heat-shock treatment. B: Analysis of the pronephric kidneys overexpressing the A263insGG HNF1β mutation Mutant animals with or without edemas developed malformations of the pronephric kidneys and the eyes. Abbreviations: e: eye; pt: proximal tubules. White arrowheads indicate more distal parts of the pronephric kidney (pronephric duct). C and D: Quantitation of the average pronephric kidney (C) and eye sizes (D) in mutant and control animals. * = p<0.01 (Student's t-test). N refers to the number of single pronephri or eyes that were measured. Scale bar = 1 mm.
A263insgg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
a263insgg - by Bioz Stars, 2026-04
93/100 stars
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fr hnf1b a263insgg plasmid 31103  (Addgene inc)
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Standard format Plasmid sent in bacteria as agar stab
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Representative larvae of a crossing between heterozygous HSPCre13 males and heterozygous A263ins6 females. The F1 offspring were heat-shocked for two hours at 34°C at early gastrula (stage 11). A: Comparison of a larva overexpressing the A263insGG HNF1β mutation with a non-transgenic control. Abbreviations: a: thickened anus; g: abnormal gut; e: reduced eye; t: abnormal tail; bracket: edema. Red fluorescence: HSPCre13 transgene. Blue fluorescence: 328del4 transgene. HS: heat-shock treatment. B: Analysis of the pronephric kidneys overexpressing the A263insGG HNF1β mutation Mutant animals with or without edemas developed malformations of the pronephric kidneys and the eyes. Abbreviations: e: eye; pt: proximal tubules. White arrowheads indicate more distal parts of the pronephric kidney (pronephric duct). C and D: Quantitation of the average pronephric kidney (C) and eye sizes (D) in mutant and control animals. * = p<0.01 (Student's t-test). N refers to the number of single pronephri or eyes that were measured. Scale bar = 1 mm.

Journal: PLoS ONE

Article Title: Heat-Shock Mediated Overexpression of HNF1β Mutations Has Differential Effects on Gene Expression in the Xenopus Pronephric Kidney

doi: 10.1371/journal.pone.0033522

Figure Lengend Snippet: Representative larvae of a crossing between heterozygous HSPCre13 males and heterozygous A263ins6 females. The F1 offspring were heat-shocked for two hours at 34°C at early gastrula (stage 11). A: Comparison of a larva overexpressing the A263insGG HNF1β mutation with a non-transgenic control. Abbreviations: a: thickened anus; g: abnormal gut; e: reduced eye; t: abnormal tail; bracket: edema. Red fluorescence: HSPCre13 transgene. Blue fluorescence: 328del4 transgene. HS: heat-shock treatment. B: Analysis of the pronephric kidneys overexpressing the A263insGG HNF1β mutation Mutant animals with or without edemas developed malformations of the pronephric kidneys and the eyes. Abbreviations: e: eye; pt: proximal tubules. White arrowheads indicate more distal parts of the pronephric kidney (pronephric duct). C and D: Quantitation of the average pronephric kidney (C) and eye sizes (D) in mutant and control animals. * = p<0.01 (Student's t-test). N refers to the number of single pronephri or eyes that were measured. Scale bar = 1 mm.

Article Snippet: Plasmid pLCMV:ECFP(loxP)(FRT)HNF1βP328L329del or –A263insGG (Addgene plasmid 31440) is composed of the CMV-driven ECFP gene flanked by loxP and FRT sites followed by the open reading frame of the human HNF1β sequence containing the mutant P328L329del or A263insGG , .

Techniques: Comparison, Mutagenesis, Transgenic Assay, Control, Fluorescence, Quantitation Assay