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Image Search Results
Journal: Frontiers in Immunology
Article Title: Functional Analysis of Variants in Complement Factor I Identified in Age-Related Macular Degeneration and Atypical Hemolytic Uremic Syndrome
doi: 10.3389/fimmu.2021.789897
Figure Lengend Snippet: Systemic complement levels in CFI rare variant carriers. (A) FI, (B) FH, and (C) C3bBbP plasma concentrations and (D) the amount of iC3b generated in the C3b degradation assay were determined with ELISA. The C3b degradation was performed three times, and iC3b levels were determined in duplicate. Dotted lines indicate the mean concentrations ± 2SD determined in non-carriers. The mean ± 2SD is (A) 57 µg/ml ± 11 µg/ml for FI, (B) 390 µg/ml ± 64 µg/ml for FH, (C) 12.9 CAU/ml ± 4.1 CAU/ml for C3bBbP, and (D) 8.5 µg/ml ± 1.6 µg/ml for generated iC3b. Solid lines indicate the mean concentration for each group.
Article Snippet: Then, the samples were immediately diluted 40x in PBS with 0.02% Tween and applied to high binding microplates (Greiner, Austria) coated with mouse-anti
Techniques: Variant Assay, Clinical Proteomics, Generated, Degradation Assay, Enzyme-linked Immunosorbent Assay, Concentration Assay
Journal: Frontiers in Immunology
Article Title: Functional Analysis of Variants in Complement Factor I Identified in Age-Related Macular Degeneration and Atypical Hemolytic Uremic Syndrome
doi: 10.3389/fimmu.2021.789897
Figure Lengend Snippet: CFI variants affect C3b degradation. C3b degradation was performed and generated iC3b was measured with ELISA. 15 µg/ml C3b and 3 µg/ml FH were added to purified FI, and incubated for 3h at 37°C. Th negative control does not contain purified FI, and for the positive control 10 ug/ml serum purified FI was incubated with the C3b/FH mix. As additional control, recombinant WT FI was incubated with 15 µg/ml C3, but FH omitted (WT -FH). The amount of iC3b generated by the variants was normalized to the concentration generated by recombinantly expressed WT FI. Bars and error bars show the mean and SD, individual dots represent the individual measurements. Samples were compared to WT using one-way ANOVA followed by Dunnet’s multiple comparison test. P-values are indicated as ****<0.001.
Article Snippet: Then, the samples were immediately diluted 40x in PBS with 0.02% Tween and applied to high binding microplates (Greiner, Austria) coated with mouse-anti
Techniques: Generated, Enzyme-linked Immunosorbent Assay, Purification, Incubation, Negative Control, Positive Control, Control, Recombinant, Concentration Assay, Comparison