Journal: BMC immunology

Article Title: The potential pathogenic roles of S100A8/A9 and S100A12 in patients with MPO-ANCA-positive vasculitis.

doi: 10.1186/s12865-022-00513-4

Figure Lengend Snippet: Fig. 1 Levels of serum and urinary S100A8/A9 and S100A12 in MPO-AAV patients and normal controls. Comparison of concentrations of serum S100A8/A9 (A), serum S100A12 (B), urinary S100A8/A9 (C), and urinary S100A12 (D) between MPO-AAV patients in the active period or remission and NC. E The relationship between serum S100A8/A9 and serum S100A12 in active MPO-AAV. F The relationship between urinary S100A8/A9 and urinary S100A12 in active MPO-AAV. NC: normal controls. ns: not significant

Article Snippet: Concentrations of S100A8/A9 (439707, Biolegend) and S100A12 (CSB-E13095h, Cusabio) in serum and urine from participants were measured by ELISA according to the manufacturer’s instructions.

Techniques: Comparison

Journal: BMC immunology

Article Title: The potential pathogenic roles of S100A8/A9 and S100A12 in patients with MPO-ANCA-positive vasculitis.

doi: 10.1186/s12865-022-00513-4

Figure Lengend Snippet: Fig. 2 The correlations between serum/urinary S100A8/A9 and S100A12 and clinical parameters in 34 active MPO-AAV. A–G showed the correlations of serum S100A8/A9 and the level of MPO-ANCA (A), serum ferritin (B), CRP (C), D-Dimer (D), erythrocyte sedimentation rate (E), rheumatoid factor (F), serum albumin (G). H and I showed the correlations of serum S100A12 and the level of MPO-ANCA (H) and serum ferritin (I). J–N showed the correlations of urinary S100A8/A9 and MPO-ANCA (J), BVAS (K), serum creatinine (L), hematuria (M), and urinary NGAL (N). O Showed the correlation between urinary S100A12 and serum creatinine. BVAS: Birmingham vasculitis activity score. NGAL: neutrophil gelatinase-associated lipocalin

Article Snippet: Concentrations of S100A8/A9 (439707, Biolegend) and S100A12 (CSB-E13095h, Cusabio) in serum and urine from participants were measured by ELISA according to the manufacturer’s instructions.

Techniques: Sedimentation, Activity Assay

Journal: BMC immunology

Article Title: The potential pathogenic roles of S100A8/A9 and S100A12 in patients with MPO-ANCA-positive vasculitis.

doi: 10.1186/s12865-022-00513-4

Figure Lengend Snippet: Fig. 4 Influence of different concentrations of S100A8/A9, and S100A12 on the chemotaxis and cell death of neutrophils. A The chemotaxis index of ANCA-activated neutrophils treated with different concentrations of S100A8/A9 and S100A12. B Effects of S100A8/A9 and S100A12 on the cell death of ANCA-activated neutrophils. C–K Representative images of the flow cytometry analysis. C Neutrophils were incubated with PBS. D Neutrophils were incubated with normal-IgG. E Neutrophils were incubated with ANCA-IgG alone. F–H Neutrophils were incubated with ANCA combined with 1, 5, and 10 μg/ml S100A8/A9, respectively. I–K Neutrophils were incubated with ANCA combined with 1, 5, and 10 μg/ml S100A12, respectively. *p < 0.05, **p < 0.01, ns: not significant

Article Snippet: Concentrations of S100A8/A9 (439707, Biolegend) and S100A12 (CSB-E13095h, Cusabio) in serum and urine from participants were measured by ELISA according to the manufacturer’s instructions.

Techniques: Chemotaxis Assay, Flow Cytometry, Incubation

Journal: BMC immunology

Article Title: The potential pathogenic roles of S100A8/A9 and S100A12 in patients with MPO-ANCA-positive vasculitis.

doi: 10.1186/s12865-022-00513-4

Figure Lengend Snippet: Fig. 3 ANCA IgG stimulated neutrophils to release S100A8/A9 (A) and S100A12 (B) concentration-dependently. *p < 0.05, **p < 0.01

Article Snippet: Concentrations of S100A8/A9 (439707, Biolegend) and S100A12 (CSB-E13095h, Cusabio) in serum and urine from participants were measured by ELISA according to the manufacturer’s instructions.

Techniques: Concentration Assay

Journal: BMC immunology

Article Title: The potential pathogenic roles of S100A8/A9 and S100A12 in patients with MPO-ANCA-positive vasculitis.

doi: 10.1186/s12865-022-00513-4

Figure Lengend Snippet: Fig. 5 S100A8/A9 and S100A12 enhanced the release of IL-1β and complement activation of ANCA-stimulated neutrophils through TLR4/RAGE. A Effects of S100A8/A9 and S100A12 on the release of IL-1β from ANCA-activated neutrophils. B–D Effects of S100A8/A9 and S100A12 on the supernatant concentrations of C5a, CBb, and sC5b-9 of ANCA-activated neutrophils. *p < 0.05, **p < 0.01, ***p < 0.001, ns: not significant

Article Snippet: Concentrations of S100A8/A9 (439707, Biolegend) and S100A12 (CSB-E13095h, Cusabio) in serum and urine from participants were measured by ELISA according to the manufacturer’s instructions.

Techniques: Activation Assay

Journal: BMC immunology

Article Title: The potential pathogenic roles of S100A8/A9 and S100A12 in patients with MPO-ANCA-positive vasculitis.

doi: 10.1186/s12865-022-00513-4

Figure Lengend Snippet: Fig. 6 S100A8/A9 and S100A12 played their pro-inflammatory effects through the p38 MAPK/NF-κB p65 pathway. The full-length blot of GAPDH in A was absent for the limited exposure space. A S100A8/A9 and S100A12 induced p38 MAPK phosphorylation and NF-κB p65 expression. B The phosphorylation ratios of p38 after neutrophils were stimulated with a combination of ANCA and S100A8/A9 or S100A12. C The activation of NF-κB p65 after neutrophils were stimulated with a combination of ANCA and S100A8/A9 or S100A12. D The phosphorylation of p38 MAPK and the activation of NF-κB p65 after the blockade of TLR4 and RAGE. E The phosphorylation ratio of p38 after blocking TLR4 and RAGE. F The expression of NF-κB p65 after blocking TLR4 and RAGE. *p < 0.05, **p < 0.01, ns: not significant

Article Snippet: Concentrations of S100A8/A9 (439707, Biolegend) and S100A12 (CSB-E13095h, Cusabio) in serum and urine from participants were measured by ELISA according to the manufacturer’s instructions.

Techniques: Phospho-proteomics, Expressing, Activation Assay, Blocking Assay

Journal: BMC immunology

Article Title: The potential pathogenic roles of S100A8/A9 and S100A12 in patients with MPO-ANCA-positive vasculitis.

doi: 10.1186/s12865-022-00513-4

Figure Lengend Snippet: Fig. 7 Effects of S100A8/A9 and S100A12 on the production of ROS and NETs in AAV. A–I Representative images of influences of S100A8/A9 and S100A12 on ANCA-induced production of ROS. Neutrophils were incubated with different stimulators and analyzed using flow cytometry. A Neutrophils were incubated with PBS. B Neutrophils were incubated with normal-IgG. C Neutrophils were incubated with ANCA. D–F Neutrophils were incubated with ANCA combined with 1, 5, and 10 μg/ml S100A8/A9, respectively. G–I Neutrophils were incubated with ANCA combined with 1, 5, and 10 μg/ml S100A12, respectively. J The mean fluorescence intensity of ROS after neutrophils were incubated with different stimulators. K Effects of S100A8/A9 and S100A12 on ANCA-induced formation of NETs. NE: neutrophil elastase

Article Snippet: Concentrations of S100A8/A9 (439707, Biolegend) and S100A12 (CSB-E13095h, Cusabio) in serum and urine from participants were measured by ELISA according to the manufacturer’s instructions.

Techniques: Incubation, Flow Cytometry, Fluorescence

Journal: Arthritis Research & Therapy

Article Title: Vaspin in atherosclerotic disease and cardiovascular risk in axial spondyloarthritis: a genetic and serological study

doi: 10.1186/s13075-021-02499-7

Figure Lengend Snippet: Scatter plot of log transformed serum vaspin levels in male and female axSpA patients. Vaspin levels were log transformed for statistical analysis, given that they were not normally distributed. Individual data points are shown, with means of each group indicated by horizontal lines

Article Snippet: A commercial enzyme-linked immunosorbent assay kit was used to measure serum vaspin levels in axSpA patients (DY4410, DuoSet® ELISA, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

Techniques: Transformation Assay

Journal: Arthritis Research & Therapy

Article Title: Vaspin in atherosclerotic disease and cardiovascular risk in axial spondyloarthritis: a genetic and serological study

doi: 10.1186/s13075-021-02499-7

Figure Lengend Snippet: Scatter plot of log transformed serum vaspin levels in axSpA patients according to their obesity status: a in the whole cohort and b stratified by sex. Vaspin levels were log transformed for statistical analysis, given that they were not normally distributed. Individual data points are shown, with means of each group indicated by horizontal lines

Article Snippet: A commercial enzyme-linked immunosorbent assay kit was used to measure serum vaspin levels in axSpA patients (DY4410, DuoSet® ELISA, R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

Techniques: Transformation Assay